Additional file 5: Figure S5. of Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?

Assessing intra assay variation of the multiplex PCR assay: Correlation between the Cq values of technical replicates for each housekeeping gene for samples ran in the multiplex PCR assay, r2 represents the correlation coefficient. (PDF 34 kb

Additional file 3: Figure S3. of Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?

Investigating the relationship between elution volume, RNA concentration and yield: Geometric mean RNA yield (orange bars) and geometric mean RNA concentration (black line) across four FFPE blocks when processing 5 Οm of tissue using the RNeasy FFPE kit using varied elution volumes, error bars represent the standard error of geomean. (PDF 13 kb

Additional file 1: Figure S1. of Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?

Investigating the linearity of the multiplex PCR assay: relationship between cDNA concentration and mean Cq value for serial dilutions of a 10 ng/Οl RNA sample ran in the multiplex PCR assay. (PDF 28 kb

Additional file 6: Table S1. of Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?

Properties of the three primers and probes used in the in house multiplex PCR assay to asses RNA quality. Roche LightCycler480 PCR machine conditions: 1 pre incubation cycle at 95 °C for 10 min followed by 45 amplification cycles at 95 °C for 10 s, 60 °C for 30 s, 72 °C for 1 s before cooling to 40 °C for 30 s. (PDF 81 kb

Additional file 2: Figure S2. of Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?

Comparison of the Nanodrop and Qubit for RNA quantification: Correlation plot between RNA concentrations measured using the Nanodrop absorbance based assay and the Qubit fluorescence based assay, r2 represents the correlation coefficient, (a) Qiagen AllPrep DNA/RNA FFPE kit n = 30, (b) Qiagen RNeasy FFPE kit, n = 30, (c) Arcturus paradise plus FFPE RNA isolation kit, n = 25, (d) Maxwell 16 LEV RNA FFPE Purification kit, n = 30. (PDF 49 kb

Microfluidic Studies of CO₂ Sequestration by Frustrated Lewis Pairs

Frustrated Lewis pairs (FLPs) comprising sterically hindered Lewis acids and bases offer the capability to reversibly capture CO₂ under mild reaction conditions. The determination of equilibrium constants and thermodynamic properties of these reactions should enable assessment of the efficiency of a particular FLP system for CO₂ sequestration and provide insights for design of new, efficient formulations of FLP catalysts for CO₂ capture. We have developed a microfluidic approach to studies of FLP–CO₂ reactions, which provides their thermodynamic characterization that is not accessible otherwise. The approach enables the determination of the equilibrium reaction constants at different temperatures, the enthalpy, the entropy, and the Gibbs energy of these reactions, as well as the enhancement factor. The microfluidic methodology has been validated by applying it to the well-characterized reaction of CO₂ with a secondary amine. The microfluidic approach can be applied for fundamental thermodynamic studies of other gas–liquid reactions