Cyclopenta[<i>b</i>]benzofuran and Secodammarane Derivatives from the Stems of <i>Aglaia stellatopilosa</i>

Four new 2,3-secodammarane triterpenoids, stellatonins A–D (<b>3</b>–<b>6</b>), together with a new 3,4-secodammarane triterpenoid, stellatonin E (<b>7</b>), and the known silvestrol (<b>1</b>), 5‴-episilvestrol (<b>2</b>), and β-sitosterol, were isolated from a methanol extract of the stems of <i>Aglaia stellatopilosa</i> through bioassay-guided fractionation. The structures of the new compounds were elucidated using spectroscopic and chemical methods. The compounds were evaluated for their cytotoxic activity against three human cancer cell lines and for their antimicrobial activity using a microtiter plate assay against a panel of Gram-positive and Gram-negative bacteria and fungi

Bioactive Flavaglines and Other Constituents Isolated from <i>Aglaia perviridis</i>

Eight new compounds, including two cyclopenta­[<i>b</i>]­benzopyran derivatives (<b>1</b>, <b>2</b>), two cyclopenta­[<i>b</i>]­benzofuran derivatives (<b>3</b>, <b>4</b>), three cycloartane triterpenoids (<b>5</b>–<b>7</b>), and an apocarotenoid (<b>8</b>), together with 16 known compounds, were isolated from the chloroform-soluble partitions of separate methanol extracts of a combination of the fruits, leaves, and twigs and of the roots of <i>Aglaia perviridis</i> collected in Vietnam. Isolation work was monitored using human colon cancer cells (HT-29) and facilitated with an LC/MS dereplication procedure. The structures of the new compounds (<b>1</b>–<b>8</b>) were determined on the basis of spectroscopic data interpretation. The Mosher ester method was employed to determine the absolute configurations of <b>5</b>–<b>7</b>, and the absolute configuration of the 9,10-diol unit of compound <b>8</b> was established by a dimolybdenum tetraacetate [Mo₂(AcO)₄] induced circular dichroism procedure. Seven known rocaglate derivatives (<b>9</b>–<b>15</b>) exhibited significant cytotoxicity against the HT-29 cell line, with rocaglaol (<b>9</b>) being the most potent (ED₅₀ 0.0007 μM). The new compounds <b>2</b>–<b>4</b> were also active against this cell line, with ED₅₀ values ranging from 0.46 to 4.7 μM. The cytotoxic compounds were evaluated against a normal colon cell line, CCD-112CoN. In addition, the new compound perviridicin B (<b>2</b>), three known rocaglate derivatives (<b>9</b>,<b> 11</b>, <b>12</b>), and a known sesquiterpene, 2-oxaisodauc-5-en-12-al (<b>17</b>), showed significant NF-κB (p65) inhibitory activity in an ELISA assay