A. Canonical structure of the enJSRV provirus.

<p>The W/R variant at position 21 is indicated by a star. The 470 bp amplicon is represented by a gray bar. B. Ligation products obtained from the OLA detection assay of W21 and R21 variants. The sequence of the phosphate-modified downstream oligo is indicated in italics. The upstream oligo that detected the R21 to W21 mutation is labeled with HEX, whereas the two FAM-labeled oligos detected the R21 variant. Probes that were mismatched to the target by a single nucleotide at their junctions were not ligated. The ligation products were discriminated by size (60 nt for W21 coding sequence vs. 64 nt for R21 coding sequences) and color (HEX for W21 vs. FAM for R21). The W and R codons are underlined.</p

List of breeds analyzed. IL16 to 63 are inbred lines derived from the <i>Pre-Alpes du Sud</i> French breed.

<p>List of breeds analyzed. IL16 to 63 are inbred lines derived from the <i>Pre-Alpes du Sud</i> French breed.</p

Boxplot representation of triplicate W21/R21 ratio measurements among randomly selected 10 samples.

<p>For each sample, W21/R21 measurement was repeated three times. Each dot represents a single measurement. Numbers correspond to the samples listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041965#pone-0041965-g001" target="_blank">Figure 1</a>. NT1 and NT2 correspond to DNA extracted from two non-tumoral samples.</p

Distribution of the W21/R21 ratios between the DNA and RNA extracts from non-tumoral (respectively n = 6 and n = 5) and tumoral samples (respectively n = 7 and n = 7).

<p>Two Wilcoxon tests were used to compare the medians of W21/R21 ratios measured in DNA and RNA extracts from non-tumoral (p-value = 0.41, non significant) and tumoral samples (p-value = 0.001, highly significant).</p

Distribution of W21 variants among 41 genomes from 10 breeds and 5 inbred lines.

<p>Individuals are numbered according to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041965#pone-0041965-t001" target="_blank">Table 1</a>. The W21/R21 ratios are indicated as percentage.</p

Factors independently associated with pneumonic-type lung adenocarcinoma.

<p>Multivariate analysis was performed using log regression model including significant variables at univariate analysis with <i>p</i><0.15.</p><p>CI: Confidence Interval.</p

Exposure to livestock in the study population.

<p>Case (P-ADC) and control (non-P-ADC) groups were compared using univariate analysis with the Chi-2 test.</p