ERRα modulates expression and activity of LDH. <i>LDHA</i> and <i>LDHB</i> expression levels were measured by quantitative real-time PCR.

<p>The ratio of LDH activity to CS activity was determined under various conditions. Measurements were made 48 h after transfection or 10 days of treatment with XCT790 and results are presented relative to the control which was assigned a unit value. <i>LDHA</i> and <i>LDHB</i> expression levels, the mean <i>LDHA/LDHB</i> ratio (A) and the relative LDH activity (B) for RO82W-1 cells transfected with 50 ng ERRα or 50 ng ERRα and 50 ng PRC or empty vectors (Control). <i>LDHA, LDHB</i> expression levels and mean of the <i>LDHA/LDHB</i> ratio (C) and relative LDH activity (D) for FTC-133 cells treated with XCT790 or vehicle (Control). <i>LDHA, LDHB</i> expression levels and mean of the <i>LDHA/LDHB</i> ratio (E) and relative LDH activity (F) for FTC-133 cells transfected with control or ERRα siRNA. The results are the mean values±SD of three experiments performed in duplicate relative to controls. *: p≤0.05.</p

ERRα inhibits <i>LDHB</i> promoter activity.

<p>(A) Different construction of the human LDHB promoter reporter plasmid. (B) RO82W-1 cells were transfected with the indicated promoter constructs together with the expression plasmid of ERRα and/or PRC. Luciferase activity was determined 48 h after transfection and normalized against renilla luciferase activity. Results, presented in Relative Light Units (RLU), are the mean values±SD of three experiments performed in duplicate. <b>*</b>: p≤0.05 versus cells transfected with plasmids controls and no ERRα or PRC.</p

ERRα and PRC coregulate the cell cycle and metabolism in thyroid cells.

<p>RO82W-1 cells transfected with ERRα and PRC or empty vector (Control) and analysed 72 h after transfection. Pangenomic microarray results for metabolism genes. Gene-expression levels are grouped by class and ordered according to their mean log level of expression (A) or color-coded in the matrix from green (underexpression) to red (overexpression) (B). Ten main gene ontologies (C). Cell proliferation and lactate levels in media (D).</p