Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array

We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cassette transporters. Genetic polymorphisms have been known to influence therapeutic response and risk of disease pathologies. Genetic screening for therapeutic and diagnostic applications thus holds great promise in clinical management. The allele-specific primer extension (ASPE) reaction was used to assay 22 multiplexed SNPs for eight subjects. Comparison of the microsphere-based ASPE assay results to sequencing results showed complete concordance in genotype assignments. The Bioplex suspension array thus proves to be a reliable, cost-effective and high-throughput technological platform for genotyping. It can be easily adapted to customized SNP panels for specific applications involving large-scale mutation screening of clinically relevant markers

IONIC REGULATION AND TRANSEPITHELIAL POTENTIAL IN THE MUDSKIPPER, BOLEOPHTHALMUS BODDAERTI

Master'sMASTER OF SCIENC

Efficient and highly sensitive screen for myotonic dystrophy type 1 using a one-step triplet-primed PCR and melting curve assay

10.1016/j.jmoldx.2014.10.001Journal of Molecular Diagnostics172128-13

Enhancement of early cervical cancer diagnosis with epithelial layer analysis of fluorescence lifetime images.

This work reports the use of layer analysis to aid the fluorescence lifetime diagnosis of cervical intraepithelial neoplasia (CIN) from H&E stained cervical tissue sections. The mean and standard deviation of lifetimes in single region of interest (ROI) of cervical epithelium were previously shown to correlate to the gold standard histopathological classification of early cervical cancer. These previously defined single ROIs were evenly divided into layers for analysis. A 10-layer model revealed a steady increase in fluorescence lifetime from the inner to the outer epithelial layers of healthy tissue sections, suggesting a close association with cellular maturity. The shorter lifetime and minimal lifetime increase towards the epithelial surface of CIN-affected regions are in good agreement with the absence of cellular maturation in CIN. Mean layer lifetimes in the top-half cervical epithelium were used as feature vectors for extreme learning machine (ELM) classifier discriminations. It was found that the proposed layer analysis technique greatly improves the sensitivity and specificity to 94.6% and 84.3%, respectively, which can better supplement the traditional gold standard cervical histopathological examinations

IL-13-driven alterations in hepatic cholesterol handling contributes to hypercholesterolemia in a rat model of minimal change disease

10.1042/CS201909611342225-23

Graphical representation of allelic ratio (A) Subject 34 and (B) Subject 12

<p><b>Copyright information:</b></p><p>Taken from "Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array"</p><p></p><p>Biological Procedures Online 2007;9():27-42.</p><p>Published online Jan 2007</p><p>PMCID:PMC2211573.</p><p>Article © by the author(s). This paper is Open Access and is published in Biological Procedures Online under license from the author(s). Copying, printing, redistribution and storage permitted. Journal © 1997-2007 Biological Procedures Online.</p

Novel role of Vav1-Rac1 pathway in actin cytoskeleton regulation in Interleukin-13induced minimal change-like nephropathy

10.1042/CS20160312Clinical Science130242317-232

Distribution profiles of mean τ₂ in divided layer 1 (■), layer 2 (▲) and layer 3 (●) of epithelium against pathological states of Normal, CIN1, CIN2 and CIN3.

<p>Here, the mean τ_<b>2</b> for each pathological state was calculated from the sample pool which includes 10 normal, 8 CIN1, 6 CIN2 and 8 CIN3 cervical tissue sections and the averaged relative standard deviation (RSD) was calculated to be 15% for all categories. More details regarding error bars are elucidated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0125706#pone.0125706.s003" target="_blank">S3 Fig</a>.</p

Variation in ELM diagnostic sensitivity (black triangle) and specificity (blue square) as feature vectors include mean τ₂ values successively from epithelial surface (layer 10) to a lower layer as the cut-off layer.

<p>Optimum sensitivity (94.6%) and specificity (84.3%) were concurrently found at the cut-off layer of 6.</p