5 research outputs found

    Effect of uremic media on the activation of NFκB p65/ReIA signaling pathway.

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    <p><b>A.</b> Ratios of phosphorylated NFκB p65/ReIA relative to the total protein, obtained in EC after 1 and 15 min of incubation with pooled sera from control donors (C), and predialysis (PreD), hemodialysis (HD), and peritoneal dialysis (PD) groups of patients. Data are corrected for cell number and are represented as mean±SEM (n = 6); *p<0.05 when compared with the other groups; #p<0.05 when compared with the control group. <b>B.</b> EC pretreated (for 30 min at 37°C) with N-acetyl-leucyl-leucyl-norleucinal (ALLN, 50 µg/ml) were incubated at different time points with pooled sera from the same groups (C, PreD, HD, and PD, as indicated), and then assayed for phospho-IκBα in cytosolic fractions by Western blot analysis. Images are representative of 6 different experiments and bar diagrams represent the mean intensity of phosphorylation (*p<0.05, **p<0.001).</p

    Effect of different peritoneal dialysis fluids on the activation of p38MAPK and NFκB p65/ReIA signaling pathways.

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    <p>Levels of phosphorylated p38 MAPK (A) and NFκB p65/ReIA (B), represented as phospho-p38 MAPK and phospho-NFκB signal at 450/595 nm, were evaluated in EC after 15 min of incubation with the pooled sera from the PD group and with the different peritoneal dialysis solutions: icodextrin (Ico) (7.5%), conventional (c-PDS) and low GDP (Low GDP-PDS) peritoneal dialysis solutions (at the concentrations of glucose of 1.5%, 2.3%, and 4.25%). Data were plotted after correction for cell number and are represented as mean±SEM (n = 6); *p<0.05 and **p<0.01 when compared with basal levels of phosphorylation.</p

    Effect of uremic media on the activation of p38MAPK signaling pathway.

    No full text
    <p><b>A.</b> Ratios of phosphorylated p38 MAPK relative to the total protein, obtained in EC after 1 and 15 min of incubation with pooled sera from control donors (C), and predialysis (PreD), hemodialysis (HD), and peritoneal dialysis (PD) groups of patients. Data are corrected for cell number and are represented as mean±SEM (n = 6); *p<0.05 when compared with the other groups. <b>B.</b> EC were incubated at different time points with pooled sera from the same groups (C, PreD, HD, and PD, as indicated), and then assayed for phospho-p38 MAPK in cytosolic fractions by Western blot analysis. Images are representative of 6 different experiments and bar diagrams represent the mean intensity of phosphorylation (*p<0.05, **p<0.001).</p
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