Mononuclear cells modulate the activity of pancreatic stellate cells which in turn promote fibrosis and inflammation in chronic pancreatitis-0

<p><b>Copyright information:</b></p><p>Taken from "Mononuclear cells modulate the activity of pancreatic stellate cells which in turn promote fibrosis and inflammation in chronic pancreatitis"</p><p>http://www.translational-medicine.com/content/5/1/63</p><p>Journal of Translational Medicine 2007;5():63-63.</p><p>Published online 5 Dec 2007</p><p>PMCID:PMC2234395.</p><p></p>tin (B; VIM), CD31 (C), CD45 (D), collagen1 (E; COL) and fibronectin (F; FBN). Arrows: area around packed clusters of mononuclear cells. Arrowheads in A-C: pancreatic stellate cells (SMA- and VIM-positive, CD31-negative). Arrowhead in D: loosely distributed area of mononuclear cell infiltration. Arrowhead in E&F: areas of less intense immunoreactivities for collagen1 and fibronectin. Dotted arrows: Areas of strong collagen1 and fibronectin staining. Original magnification: ×50. (G) Arrows: areas of loosely distributed/single mononuclear cells, dotted arrows: area without mononuclear cells. (H&I) Arrows/dotted arrows: corresponding areas to (G)

Mononuclear cells modulate the activity of pancreatic stellate cells which in turn promote fibrosis and inflammation in chronic pancreatitis-3

<p><b>Copyright information:</b></p><p>Taken from "Mononuclear cells modulate the activity of pancreatic stellate cells which in turn promote fibrosis and inflammation in chronic pancreatitis"</p><p>http://www.translational-medicine.com/content/5/1/63</p><p>Journal of Translational Medicine 2007;5():63-63.</p><p>Published online 5 Dec 2007</p><p>PMCID:PMC2234395.</p><p></p> White bars: Pooled donor, CP or sepsis PBMC and corresponding PSC supernatants. Black bars: coculture supernatants. *p < 0.05 as assessed using the Mann-Whitney U test. (B) Immunoblot analysis of fibronectin in PSC cell lysates. Densitometry shown as percent of control adjusted to the corresponding gamma-tubulin density

Mononuclear cells modulate the activity of pancreatic stellate cells which in turn promote fibrosis and inflammation in chronic pancreatitis-7

<p><b>Copyright information:</b></p><p>Taken from "Mononuclear cells modulate the activity of pancreatic stellate cells which in turn promote fibrosis and inflammation in chronic pancreatitis"</p><p>http://www.translational-medicine.com/content/5/1/63</p><p>Journal of Translational Medicine 2007;5():63-63.</p><p>Published online 5 Dec 2007</p><p>PMCID:PMC2234395.</p><p></p>phaSMA- (SMA) and vimentin-positive (VIM) structures are endothelia, CD31 staining was performed. (D-F) In areas of loosely packed mononuclear cells (arrowhead in D) and packed mononuclear cells (white arrow in D), there is sparse immunoreactivity for collagen1 (arrowhead and white arrow in E) and moderate staining for fibronectin (arrowhead and white arrow in F). In areas with a low number of CD45-positive mononuclear cells (dotted arrow in D), the staining intensities for collagen1 (dotted arrow in E; COL) and fibronectin (dotted arrow in F; FBN) are stronger. Original magnification: ×200

Mononuclear cells modulate the activity of pancreatic stellate cells which in turn promote fibrosis and inflammation in chronic pancreatitis-6

<p><b>Copyright information:</b></p><p>Taken from "Mononuclear cells modulate the activity of pancreatic stellate cells which in turn promote fibrosis and inflammation in chronic pancreatitis"</p><p>http://www.translational-medicine.com/content/5/1/63</p><p>Journal of Translational Medicine 2007;5():63-63.</p><p>Published online 5 Dec 2007</p><p>PMCID:PMC2234395.</p><p></p>tin (B; VIM), CD31 (C), CD45 (D), collagen1 (E; COL) and fibronectin (F; FBN). Arrows: area around packed clusters of mononuclear cells. Arrowheads in A-C: pancreatic stellate cells (SMA- and VIM-positive, CD31-negative). Arrowhead in D: loosely distributed area of mononuclear cell infiltration. Arrowhead in E&F: areas of less intense immunoreactivities for collagen1 and fibronectin. Dotted arrows: Areas of strong collagen1 and fibronectin staining. Original magnification: ×50. (G) Arrows: areas of loosely distributed/single mononuclear cells, dotted arrows: area without mononuclear cells. (H&I) Arrows/dotted arrows: corresponding areas to (G)

Mononuclear cells modulate the activity of pancreatic stellate cells which in turn promote fibrosis and inflammation in chronic pancreatitis-1

<p><b>Copyright information:</b></p><p>Taken from "Mononuclear cells modulate the activity of pancreatic stellate cells which in turn promote fibrosis and inflammation in chronic pancreatitis"</p><p>http://www.translational-medicine.com/content/5/1/63</p><p>Journal of Translational Medicine 2007;5():63-63.</p><p>Published online 5 Dec 2007</p><p>PMCID:PMC2234395.</p><p></p>phaSMA- (SMA) and vimentin-positive (VIM) structures are endothelia, CD31 staining was performed. (D-F) In areas of loosely packed mononuclear cells (arrowhead in D) and packed mononuclear cells (white arrow in D), there is sparse immunoreactivity for collagen1 (arrowhead and white arrow in E) and moderate staining for fibronectin (arrowhead and white arrow in F). In areas with a low number of CD45-positive mononuclear cells (dotted arrow in D), the staining intensities for collagen1 (dotted arrow in E; COL) and fibronectin (dotted arrow in F; FBN) are stronger. Original magnification: ×200