16 research outputs found
Vitality and in vitro pollen germination of different ‘Oblačinska’ sour cherry clones
Vitality of pollen, in vitro pollen germination and pollen tube growth
(pollen tube length and pollen tube growth rate) were investigated in
Oblačinska sour cherry in order to determine the differences between clones
which have divergent yielding potential. For this purpose two ‘Oblačinska’
sour cherry clones with high fruit set and high yields (II/2, III/9) and two
with low fruit set and low-yielding (XI/3 and XIII/1) were used in this
study. Pollen germination was done on artificial medium containing 14%
sucrose and 0.3% agar-agar at room temperature (23°C). Pollen tube growth was
stopped with a drop of 40% formaldehyde, 1, 3, 6, 12 and 24 h after contact
with the medium. The maximum percentage of germination ranged from 13.01%
(clone II/2, after 1 h) to 54.19% (clone III/9, after 24 h). Pollen tube
length varied from 64.84 μm (clone XIII/1, after 1 h) to >1,100 μm (clones
II/2 and III/9, after 24 h). Pollen growth rate was quite high (up to 1.71 μm
min-1) after 6 h of germination, but rather decreasing until 24 h of
germination (0.56-0.83 μm min-1). The dynamics of in vitro pollen tubes
growth among the clones were quite different, especially after 12 h and 24 h
of germination. Clones that are singled out as fruitful (II/2 and III/9) gave
much better results regarding pollen germination and pollen tube growth in
comparison to clones which were characterized by low fruit set and yields
(XI/3 and XIII/1). [Project of the Serbian Ministry of Education, Science and
Technological Development, Grant no. TR 31063