L‑arginine‑containing mesoporous silica nanoparticles embedded in dental adhesive (Arg@MSN@DAdh) for targeting cariogenic bacteria

Dental caries is the major biofilm-mediated oral disease in the world. The main treatment to restore caries lesions consists of the use of adhesive resin composites due to their good properties. However, the progressive degradation of the adhesive in the medium term makes possible the proliferation of cariogenic bacteria allowing secondary caries to emerge. In this study, a dental adhesive incorporating a drug delivery system based on L-arginine-containing mesoporous silica nanoparticles (MSNs) was used to release this essential amino acid as a source of basicity to neutralize the harmful acidic conditions that mediate the development of dental secondary caries. The in vitro and bacterial culture experiments proved that L-arginine was released in a sustained way from MSNs and diffused out from the dental adhesive, effectively contributing to the reduction of the bacterial strains Streptococcus mutans and Lactobacillus casei. Furthermore, the mechanical and bonding properties of the dental adhesive did not change significantly after the incorporation of L-arginine-containing MSNs. These results are yielding glimmers of promise for the costeffective prevention of secondary caries.Spanish GovernmentEuropean CommissionRey Juan Carlos University CTQ2017-88642-R PID2021-125216OB-I00 2022/00004/01

Long-term sealing ability of GuttaFlow versus Ah Plus using different obturation techniques

Objective. To compare the long-term sealing ability of GuttaFlow® using different obturation techniques. Study Design. Three hundred teeth, prepared with a crown-down technique, were divided into thirty experimental groups (n=10) to evaluate the apical and coronal leakage, at 3, 30 and 120 days, of lateral compaction gutta-percha + AH Plus?, lateral compaction gutta-percha + GuttaFlow®, single cone + AH Plus?, single cone + GuttaFlow®, and GuttaFlow® only. Results. Both coronal and apical leakage, at the three times of measurement, no significant differences were found among GuttaFlow® + lateral compaction gutta-percha and GuttaFlow® + single cone groups, whereas the only GuttaFlow® reached the highest leakage values at 30 and 120 days. AH Plus?, using both techniques, showed high levels of leakage after 120 days to the coronal leakage and after 30 days to the apical leakage when compared silicon based sealer. Conclusion. GuttaFlow®, using with lateral compaction and single cone techniques, shows a greater apical and coronal sealing ability than AH Plus? over time. GuttaFlow® when used as only creates a poorer sealing than when used with lateral compaction gutta-percha or single cone techniques

Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy

Aim: To evaluate the cytotoxicity of 15% citric acid, 5% phosphoric acid and 2.5% NaOCl on cultured fibroblasts using MTT colorimetric assay. Methodology: Irrigating solutions of 5% phosphoric acid, 15% citric acid, and 2.5% NaOCl, diluted at 0.1% and 0.5%, were applied to cell cultures of 3T3L1 fibroblasts. The cell viability was determined by means of MTT colorimetric assay after a period of 1, 6 and 24 hours. Percentages of cell viability were analyzed using the Kruskal-Wallis test for global comparisons and the Mann-Whitney U-test for pairwise comparisons. Results: The percentage of cell viability diminished progressively over a 24 hour period in all solutions at both dilutions. At 0.1% dilution, 2.5% NaOCl (63.39%) and 15% citric acid (53.91%) showed the highest percentage of cell viability (p=0.083). At 0.5% dilution, 2.5% NaOCl again showed the highest cell viability value (48.51%). Conclusions: The irrigating solution with the highest percentage of cell viability was 2.5% NaOCl at both 0.1% and 0.5% dilutions. A very low percentage of cell viability was obtained with 15% citric acid and 5% phosphoric acid at 0.5% dilution

Efficacy of mixed diclofenac solutions against root canal biofilms

Research Group CTS- 167 of the Junta de Andalucia, SpainThe objective of this research was to evaluate the efficacy of diclofenac sodium solutions, with or without cetrimide (CTR) added, against polymicrobial root canal biofilms grown in dentin specimens. The study groups were: (1) 5% diclofenac sodium (DCS); (2) 2.5% DCS; (3) 2.5% DCS + 0. 2% CTR; (4) 2.5% DCS + 0.4% CTR and (5) 0.9% saline solution (SS) as the control. After 5 min of solution contact with the biofilms, the antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay as well as confocal laser scanning microscopy (CLSM). Microbial quantification was indicated as the percentage reduction of relative light units (RLUs) for the ATP assay, the Log(10) total biovolume and the viability percentage (green cells) for CLSM. Solutions of 2.5% DCS + 0.4% CTR and 5% DCS showed the highest antimicrobial efficacy. Cetrimide increased the antibiofilm activity of diclofenac sodium against endodontic biofilms.Junta de Andalucía CTS- 16

Antibiofilm Activity of Diclofenac and Antibiotic Solutions in Endodontic Therapy

Introduction: The aim of this study was to compare the antibiofilm effects of a triple antibiotic solution (TAS); a double antibiotic solution (DAS); and 5%, 2.5%, and 1.25% diclofenac solutions (DCSs) against Enteroccocus faecalis biofilm. Methods: Eighty-four sterile radicular dentin blocks were used as biofilm substrate for 3 weeks. The study groups were as follows: (1) 1 mg/mL TAS (minocycline, metronidazole, and ciprofloxacin), (2) 1 mg/mL DAS (metronidazole and ciprofloxacin), (3) 5% DCS, (4) 2.5% DCS, (5) 1.25% DCS, and (6) 0.9% saline solution. The antimicrobial activity was evaluated by bacterial count determinations and confocal laser scanning microscopy. The contact time for the antimicrobial tests was 5 minutes. Bacterial counts were expressed as the reduction percentage of colony-forming units; for the confocal laser scanning microscopic evaluation, the log10 total biovolume and percentage of green population (live cells) were calculated. Results: The colony-forming unit reduction percentage ranged between 62.98 and 98.62, respectively, for TAS and 5% DCS. The DCS showed a concentration-dependent effect.For the confocal laser scanning microscopy, the log10 total biovolume in all groups was very similar and showed a scarce (1.39-1.02) but significant reduction with respect to the control; 5% and 2.5% DCSs gave the lowest viable cell percentage. The TAS and DAS groups showed intermediate values without significant differences between them. Conclusions: DCSs at 5% and 2.5% have greater antimicrobial effects than TAS and DAS and may be considered a valid alternative for controlling the infection of teeth with apical periodontitis.Grupo CTS 16

Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms

Persistent infections have become a challenge in dentistry because of growing antibiotic resistance. Nonsteroidal anti-inflammatory drugs (NSAIDs) appear to be a therapeutic alternative to control biofilm infection. The objective of this work is to evaluate the antimicrobial activity and cytotoxicity of sodium diclofenac (DCS), ibuprofen (IBP) and ibuprofen arginine (IBP-arginine) solutions against endodontic polymicrobial biofilms. Sterile radicular dentin blocks of 4 mm 4 mm 0.7 mm were used as substrate to grow biofilm. The dentin blocks were submerged into solutions for 5 min. The antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay and confocal laser scanning microscopy (CLSM). Fibroblasts 3T3-L1 (ECACC 86052701) were used to test the cytotoxicity of irrigating solutions. The antibiofilm effects determined by the ATP assay showed that 4% IBP-arginine solution exerted the highest antibiofilm activity, followed by 4% DCS and 4% IBP, with statistical differences among groups (p < 0.001). As for CLSM, 4% DCS and 4% IBP-arginine solutions gave the lowest viable cell percentages, without significant differences between them. Cytotoxicity results at 1/10 dilution were similar for all solutions. At 1/100 dilution, a 4% DCS solution obtained the lowest cell viability for both time periods assayed, 1 h and 24 h. The IBP-arginine group showed the highest cell viability at 24 h. In this preliminary study, in terms of antibiofilm activity and cytotoxicity, a mixed 4% IBP-arginine solution gave the most promising results. NSAID solutions could be recommendable drugs for endodontic disinfection proceduresResearch Group CTS-167 of Junta de Andalucía, Spain

Influence of adhesive systems on microtensile bond strength of resin-based endodontic sealers to the root dentin

Objective: The aim of this study was to determine the microtensile bond strength to root dentin of AH PlusTM and EndoREZ® with Clearfil Liner Bond 2V and Optibond SoloTM Plus adhesive systems. Study Design: The coronal and middle thirds of six single rooted bovine teeth was split longitudinally in a mesio- distal direction. The two halves were joined with AH Plus or EndoREZ, with and without the use of Clearfil Li - ner Bond 2V and Optibond SoloTM Plus adhesive systems. Build-ups were vertically sectioned into quadrangular (?1mmx1mm) compound bars and subjected to tensile tests at a constant crosshead speed (1 mm/min) until debon - ding. Results: Optibond ® Solo PlusTM in combination with AH PlusTM and EndoREZ ® showed the highest mean micro - tensile bond strength values, in both coronal and middle thirds. The lowest results were seen in the groups where no dentine adhesive was applied, and in those where the self-etching adhesive Clearfil Liner Bond 2V was used. Conclusion: The microtensile bond strength to root dentin of AH PlusTM and EndoREZ may be increased with the use of a total-etch adhesive

Apical seal comparison of low-temperature thermoplasticized gutta-percha technique and lateral condensation with two different master cones

Objetivo: comparar el sellado apical en conductos mesio-vestibulares de molares obturados con gutapercha termoplastificada a baja temperatura o con técnica de condensación lateral usando un cono maestro de gutapercha de conicidad .06 o .02. Como objetivo secundario se evaluó la penetración del espaciador en los conductos cuando se utilizó un cono de conicidad .02 o .06. Metodología: cuarenta y cuatro conductos mesio-vestibulares curvos (25-40º) fueron preparados con instrumentos rotatorios del niquel-titanio de conicidad .06 y distribuidos aleatoriamente en dos grupos control (n=4) y tres grupos experimentales (n=12) para obturarlos con el sistema Ultrafil® 3D o técnica de condensación lateral de gutapercha en frío con conos maestros de conicidad .06 o.02. AH-Plus fue utilizado como cemento sellador. La profundidad de penetración del espaciador fue registrada en milímetros. Las raíces fueron cubiertas con dos capas de barniz de uñas, sumergidas en tinta china durante 7 días, seccionadas transversalmente y examinadas con un estereomicroscopio. Para determinar si existían diferencias en la penetración del espaciador entre grupos se utilizó el test de la T de Student. La prueba de Kruskal-Wallis fue utilizada para determinar si existían diferencias en la penetración del tinte. Resultados: no hubo diferencias en la microfiltración entre los tres grupos de estudio (p= 0.396), que mostraban una media muy similar (0.42. 0.75 y 0.42). La comparación de la profundidad de penetración del espaciador en los grupos obturados mediante condensación lateral fue significativamente superior cuando se usó un cono de conicidad .02 (p= 0.001). Conclusión: el sistema Ultrafil®3D y la técnica de condensación lateral de la gutapercha con conos maestros de conicidad .06 o .02 fueron igualmente eficaces en el sellado apical de conductos curvos. El espaciador penetró en el conducto significativamente más cuando se empleó un cono de conicidad .02

Decalcifying effects of antimicrobial irrigating solutions on root canal dentin

Objective: The aim of this study was to determine the decalcifying efficacy of 7% maleic acid (MA), 2% chlorhexidine (CHX), and combinations of 7% MA + 0.2% cetrimide (CTR) and 2% CHX + 0.2% CTR, in four time periods. Study Design: Four specimens per tooth were obtained from a 2-mm thick slice of the cervical third of the root of ten human incisors. At 1, 2, 3 and 5 minutes of immersion, the concentrations of Ca2+ were measured by atomic absorption spectrophotometry. The results were analyzed using the Mann-Whitney U-test. Results: Statistically significant differences were seen for the extracted calcium in all time periods. The amount of calcium extracted by 7% MA was the highest at all four immersion times, followed by 7% MA + 0.2% CTR. Two percent CHX and its combination with 0.2% CTR extracted virtually no calcium. Conclusions: The decalcifying capacity of 7% MA and 2% CHX diminished when combined with 0.2% CTR

Ex vivo study of bacterial coronal leakage in indirect pulp treatment

Objective: The aim of this study was to evaluate, ex vivo, bacterial coronal leakage with different antimicrobial agents applied to the dentine for indirect pulp treatment (IPT). Study D esign: Sixty extracted teeth were prepared and randomly distributed into 5 groups (n=10): Group 1: no antimicrobial dentine treatment; group 2: 1% chlorhexidine (CHX)+1% thymol varnish (Cervitec ®) ; group 3: 2 % CHX solution; group 4: 40% CHX varnish (EC40TM) and group 5: ClearfilTM Protect Bond (CPB). Ten teeth served as controls. The teeth were restored using a resin-modified glass ionomer cement (GIC) and then mounted in a two-chamber device. The coronal access was exposed to Streptococcus mutans for 45 days. The appearance of turbidity in the BHI broth of the lower chamber was considered as specimen leakage. Results: Survival analysis, determined by non parametric Kaplan-Meier and log-rank tests, showed that the best results were for groups EC40TM+GIC and GIC alone; yet there were not statistically significant differences be - tween them. All specimens of CPB+GIC and 2% CHX+GIC, leaked at 45 days. Conclusions: In IPT the use of GIC without pretreatment of the dentine and pretreatment with 40% CHX varnish resulted in a significant delay of bacterial coronal leakage