Logistic regression to examine the interaction of low <i>miR-16</i> and low <i>miR-21</i> expression on the association with SGA status.

<p>Also included in model: Relative Weight Gained During Pregnancy, Maternal Ethnicity, Maternal Age, Delivery Method, Insurance, and Infant Gender.</p><p>p for trend (p<0.02).</p><p>Samples lacking one or more piece of covariate data were excluded from the model.</p

Expression of <i>miR-16</i>, <i>miR-21</i>, <i>miR-93</i>, <i>miR-135b</i>, <i>miR-146a</i>, and <i>miR-182</i> determined through qRT-PCR in 107 primary human term placenta samples.

<p>Expression of <i>miR-16</i>, <i>miR-21</i>, <i>miR-93</i>, <i>miR-135b</i>, <i>miR-146a</i>, and <i>miR-182</i> determined through qRT-PCR in 107 primary human term placenta samples.</p

TCL-1 cells overexpressing <i>miR-21</i> express approximately 50% less PTEN protein than TCL-1 cells transfected with negative control (p<0.05).

<p>TCL-1 cells overexpressing <i>miR-21</i> express approximately 50% less PTEN protein than TCL-1 cells transfected with negative control (p<0.05).</p

Demographics of the study population (n = 107).

<p>*One sample was missing mode of delivery data.</p><p>**Three samples were missing maternal ethnicity data.</p><p>***Two samples were missing maternal cigarette smoking during pregnancy data.</p><p>****Two samples were missing weight gained data.</p><p>Note: Tests for the difference in specific clinical or demographic factors between the 2 groups (SGA and non-SGA). T-test was used to examine differences in continuous variables, and χ2-tests for categorical variables.</p

Logistic regression for the association between individual miRNA expression and SGA status.

<p>Also included in models: Relative Weight Gained During Pregnancy, Maternal Ethnicity, Maternal Age, Delivery Method, Insurance, and Infant Gender.</p><p>Samples lacking one or more piece of covariate data were excluded from the model.</p

Distribution of infant birthweights (y-axis) by primary term human placenta miRNA expression quartiles (x-axis).

<p>(A) <i>miR-16</i> (p = 0.04), (B) <i>miR-21</i> (p = 0.02), (C) <i>miR-93</i> (p = 0.88), (D) <i>miR-135b</i> (p = 0.84), (E) <i>miR-146a</i> (p = 0.46), and (F) <i>miR-182</i> (p = 0.55). Black bars indicate median of birthweight percentile within each quartile. Kruskal-Wallis tests revealed that birthweight percentile significantly differed across quartiles of <i>miR-16</i> and <i>miR-21</i> expression (p<0.05).</p

Population Demographics, Clinical Characteristics, and NNNS Summary Scores.

<p>Note: Not all N values equal 186 because of missing values.</p

Bivariate Correlations Between HSD11B2 Mean Methylation Extent and NICU Network Neurobehavioral Scales Summary Scores.

<p>Bivariate Correlations Between HSD11B2 Mean Methylation Extent and NICU Network Neurobehavioral Scales Summary Scores.</p

Generalized Linear Models of the Association between HSD11B2 Methylation Extent and NICU Network Neurobehavioral Scales Quality of Movement, Attention, and Hypertonicity Scales.

<p>Generalized Linear Models of the Association between HSD11B2 Methylation Extent and NICU Network Neurobehavioral Scales Quality of Movement, Attention, and Hypertonicity Scales.</p

Plots of the relationships of <i>HSD11B2</i> mean methylation and selected NICU Network Neurobehavioral Scales.

<p>Scatterplots of the correlations between <i>HSD11B2</i> mean methylation extent (x-axis) and infant NICU Network Neurobehavioral Scales (A) Quality of Movement, (B) Attention, and (C) Hypertonicity scores (y-axes). Values of the Spearman correlation coefficient (ρ ) and its P-value are presented with each plot.</p