Adventure Pin Collection

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The Matching Sweatpants Story

So, my friend Ashley’s sister’s friend, who is a girl, was going on a first date with a guy. For the date, the guy picked her up and they went to a popular hiking trail that had a gift shop at the head of the trail. So before they even start the hike the guy is feeling uncomfortable because, well because he needed to fart. Farting isn’t the best thing to do on your first date with a girl. But he really had to fart so we slowly let it out, or at least he tried to. You see it was then he realized that “oh no!” that wasn’t just a fart… it was a shart… yeah he had just accidently crapped his pants, so here he is on a date with a bunch of crap in his underwear, and the poor dude was wearing kakis so there wasn’t much hope for him, he had to think fast. That is when he saw the gift shop and had a great idea! He told his date that they should go buy matching sweat pants. The girl thought this was a really weird first date activity, but didn’t really have a reason to say no, so they looked around and bought some sweatpants the came in a bag, if I remember correctly they had trains on them, but I am not really sure. The guy had the idea that they should both change into them, which again, the girl thought was pretty weird, I mean they were supposed to hike, so sweatpants would be weird, but again she didn’t want to say no. They went over to an outhouse which was the only available restroom and the boy said that we would go first. He got in and finally he could take off his spoiled underwear and shorts. He took one look at them and decided that they were past saving so he just stuffed them into the toilet hole, and watch them fall into the nastiness that was below. Then he went to open the sweatpants he had bought. He pulled off the plastic and to his horror, the “sweatpants” they had bought were actually sweatshirt. He quickly does a double take looking deep in the pit of poop and then realizes the predicament he was in. He is too embarrassed to confront the girl so he puts one leg though each arm of the sweatshirt and holds it up with his hands and runs out of the outhouse to his car and drives away, without saying a word to the girl, who had no idea what was going on. The girl eventually called her parents to pick her up. She didn’t hear from the runaway date for weeks but eventually he did tell her what actually happened that day

The Wall Joke

What do Pink Floyd and Princess Diana have in common? The Wall was their last greatest hit

3 Legged Dog Joke

What do you call a 3 legged dog? Target Practic

It Sure is Dark in Here Joke

So one day this little boy is home, he walks in his mother and a man having an affair, he hides in the closet to watch them, as they don’t notice him. The boy’s father comes home, the mother pushes the man into the closet with the little boy not knowing he is already in there. The boy says “it sure is dark in here” the man replies “yes it is.” The boy tells the man, “I have a baseball glove,” the man replies “okay how much?” The little boy says $750. The man tells him that it is too much. The little boy says “my father is right outside”. The man response “okay” and hands over the money. The little boy gives him the glove. A few days pass and the same thing happens, the little boy says, “it sure is dark in here” the man replies “yes it is.” The little boy says “I have a baseball.” Remembering what happened last time the man asks “how much?” the little boy says$250. The man hands over the money gets the baseball and eventually they leave. A few days later the boy’s father tells him to go get his mitt and his ball so they can play catch the little boy says he can’t because he sold the mitt and the ball to his friends. The boy’s father asks how much he sold him for. The boy tells him $1000. The boy’s father says he overcharged his friends and that he needs to go confess about this. He takes him to the church and sends the little boy to the confessional booth. After a moment of silence the little boy looks around “it sure is dark in here.” The priest says “not this time you little shit, this time you are in my closet.

Dark Humor Joke

Dark Humor is like food, Not everyone gets it

Donald Trump Joke

Donald Trump is our Presiden

Structure of the γ-D-glutamyl-L-diamino acid endopeptidase YkfC from Bacillus cereus in complex with L-Ala-γ-D-Glu: insights into substrate recognition by NlpC/P60 cysteine peptidases.

Dipeptidyl-peptidase VI from Bacillus sphaericus and YkfC from Bacillus subtilis have both previously been characterized as highly specific γ-D-glutamyl-L-diamino acid endopeptidases. The crystal structure of a YkfC ortholog from Bacillus cereus (BcYkfC) at 1.8 Å resolution revealed that it contains two N-terminal bacterial SH3 (SH3b) domains in addition to the C-terminal catalytic NlpC/P60 domain that is ubiquitous in the very large family of cell-wall-related cysteine peptidases. A bound reaction product (L-Ala-γ-D-Glu) enabled the identification of conserved sequence and structural signatures for recognition of L-Ala and γ-D-Glu and, therefore, provides a clear framework for understanding the substrate specificity observed in dipeptidyl-peptidase VI, YkfC and other NlpC/P60 domains in general. The first SH3b domain plays an important role in defining substrate specificity by contributing to the formation of the active site, such that only murein peptides with a free N-terminal alanine are allowed. A conserved tyrosine in the SH3b domain of the YkfC subfamily is correlated with the presence of a conserved acidic residue in the NlpC/P60 domain and both residues interact with the free amine group of the alanine. This structural feature allows the definition of a subfamily of NlpC/P60 enzymes with the same N-terminal substrate requirements, including a previously characterized cyanobacterial L-alanine-γ-D-glutamate endopeptidase that contains the two key components (an NlpC/P60 domain attached to an SH3b domain) for assembly of a YkfC-like active site

The structure of BVU2987 from Bacteroides vulgatus reveals a superfamily of bacterial periplasmic proteins with possible inhibitory function.

Proteins that contain the DUF2874 domain constitute a new Pfam family PF11396. Members of this family have predominantly been identified in microbes found in the human gut and oral cavity. The crystal structure of one member of this family, BVU2987 from Bacteroides vulgatus, has been determined, revealing a β-lactamase inhibitor protein-like structure with a tandem repeat of domains. Sequence analysis and structural comparisons reveal that BVU2987 and other DUF2874 proteins are related to β-lactamase inhibitor protein, PepSY and SmpA_OmlA proteins and hence are likely to function as inhibitory proteins

Structures of three members of Pfam PF02663 (FmdE) implicated in microbial methanogenesis reveal a conserved α+β core domain and an auxiliary C-terminal treble-clef zinc finger

The first structures from the FmdE Pfam family (PF02663) reveal that some members of this family form tightly intertwined dimers consisting of two domains (N-terminal α+β core and C-terminal zinc-finger domains), whereas others contain only the core domain. The presence of the zinc-finger domain suggests that some members of this family may perform functions associated with transcriptional regulation, protein–protein interaction, RNA binding or metal-ion sensing