72 research outputs found

    GSTP1 methylation analysis of DNA samples (PC study) tested in duplicate with PyroMark CpG mode and AQ mode.

    No full text
    <p>DNA samples (N = 36) obtained from paraffin embedded prostate tumor tissue of patients involved in “PC study” [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150483#pone.0150483.ref024" target="_blank">24</a>]. Mean methylation percentage of 4 CpGs in the promoter of GSTP1 gene is reported. Target sequence: 72 bp of GSTP1 promoter (Gene Bank M24485 at position 1001–1072); CpGs at position 1038, 1040, 1043, 1049. Deviation between paired samples ranged from 0 to 7.15%. One red score was assigned by the CpG mode to the sample indicated by the arrow. No sample has a red score assigned by the AQ mode.</p

    MGMT methylation analysis of DNA samples (DD study) tested in duplicate onto PyroMark CpG mode and AQ mode.

    No full text
    <p>DNA samples (N = 28) obtained buffy coats of patients involved in “DD study” [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150483#pone.0150483.ref025" target="_blank">25</a>]. Mean methylation percentage of 6 CpGs in the promoter of MGMT gene is reported. Target sequence: 194 bp of MGMT (Gene ID 4255 at position 44526–44719); CpGs at position 44600, 44604, 44607, 44614, 44621, 44623. Deviation between paired samples ranged from 0.3 to 14.4%. The CpG mode assigned 14 red score. No sample has a red score assigned by the AQ mode.</p

    Childhood Cancer Registry of Piedmont 1967–2011: Data quality indicators by calendar period.

    No full text
    <p>Total number of cases per period (N), cases (number and percentage) identified through death certificate only (DCO), microscopically verified (MV) cases, cases with ill-defined or unspecified site (ICD-10: C26, C39, C48, C76, C80), cases based on imaging only, by calendar period of registration.</p

    Pyrosequencing primers and annealing temperature.

    No full text
    <p>* Target: 194 bp of MGMT (Gene ID 4255 at position 44526–44719) promoter sequence including 6 CpGs at position: 44600, 44604, 44607, 44614, 44621, 44623.</p>§<p> Target: 136 bp of RASSF1A (Gene ID 11186 at position 1697–1833) promoter sequence including 6 CpGs at position: 1746, 1750, 1755, 1757, 1767, 1780.</p>#<p> Target: 108 bp of LINE-1 (GeneBank X58075.1 at position 117–224) promoter sequence including 6 CpGs at position: 156, 131, 165, 167, 172, 182.</p><p>Pyrosequencing primers and annealing temperature.</p
    • …
    corecore