Additional file 1: of Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria

Nucleic acid amplification techniques and samples used for atypical bacteria detection. (DOCX 39 kb

Alphabet de la grande guerre / André Hellé

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Rickettsial infection in ticks collected from humans and domestic animals.

<p>Rickettsial infection in ticks collected from humans and domestic animals.</p

Bayesian phylogenetic analysis of 12S mtDNA (HKY+I+G model).

<p>Bayesian phylogenetic analysis of 12S mtDNA (HKY+I+G model).</p

Multivariate mixed models for rickettsial seropositivity.

<p>Multivariate mixed models for rickettsial seropositivity.</p

Bayesian phylogenetic analysis of <i>ompA</i> (TPM3uf+I model).

<p>Bayesian phylogenetic analysis of <i>ompA</i> (TPM3uf+I model).</p

Results of IgG antibodies titers against <i>R</i>. <i>rickettsii</i>, <i>R</i>. <i>parkeri</i> y <i>R</i>. <i>amblyommatis</i> antigens in equine and canine serum samples.

<p>Results of IgG antibodies titers against <i>R</i>. <i>rickettsii</i>, <i>R</i>. <i>parkeri</i> y <i>R</i>. <i>amblyommatis</i> antigens in equine and canine serum samples.</p

Bayesian phylogenetic analysis of <i>gltA</i> (TPM1uf model).

<p>Bayesian phylogenetic analysis of <i>gltA</i> (TPM1uf model).</p

The levels of antibody responses against <i>P. vivax</i> erythrocytic antigens differ among different Duffy genotypes.

<p>Immune recognition of <i>P. vivax</i> sporozoite antigen (A: PvCSP) and blood stage antigens (B: PvMSP1, C: PvDBP), or <i>P. falciparum</i> sporozoite antigen (D: PfCSP) and blood stage antigen (E: PfMSP1) by sera of donors with different Duffy genotypes FY*A/FY*B (A/B), FY*A/FY*Bnull (A/Bnull), FY*B/FY*B (B/B) and FY*B/FY*Bnull (B/Bnull). Values are expressed as Index of Reactivity (IR) that is calculated as test OD divided by cut-off OD. The cut-off OD is the average OD value of US donors (with no prior exposure to malaria)+3 SD. The IR of donors with FY*A/FY*B genotype is compared with those of FY*A/FY*Bnull, and FY*B/FY*B with FY*B/FY*Bnull using Student's <i>t</i>-test and <i>p</i> values<0.05 were considered significant.</p

<i>P. vivax</i> erythrocytic antigen specific IgM and IgG subclass profiles among different Duffy genotypes.

<p>IgM and IgG subclass among Fy+ donors that had total IgG against PvMSP1 and PvDBP was measured by ELISA to determine the frequencies (A) and magnitude (B) of responses against PvMSP1 and PvDBP. IgM and IgG subclass against PvMSP1 and PvDBP were also compared among donors with different Duffy genotypes (C). Samples are considered positive when the OD reading is > average OD of naïve controls + 3SD for frequency of responses or index of reactivity (IR) is greater than 1 for the magnitude of responses, respectively.</p