Liver MDA and Lung microvascular permeability (EBD).

<p>3A –Liver MDA. Data are expressed as mean values * (SAL vs TD and SAL vs CONTR) p<0.05; 3B—Lung microvascular permeability (EBD) *(CONT vs SAL and CONTR vs TD) p<0.05. (TD vs SAL) NS. CONTR—Control Group; SAL (n = 10)–Saline Group (n = 15); TD—Trisulfated Disaccharide Group (n = 15). NS: No Significance.</p

Aminotransferases four hours after liver reperfusion.

<p>1A –AST—aspartate aminotransferase; 1B –ALT—alanine aminotransferase CONTR—Control Group (n = 10); SAL—Saline Group (n = 15); TD—Trisulfated Disaccharide Group (n = 15). Data are expressed as mean values * (SAL vs TD and SAL vs CONTR) p<0.05.</p

Liver histology.

<p>Liver cell necrosis was significantly lower in the TD group when compared to the SAL group. 5A– Control Group (n = 10); 5B –Saline Group (n = 15); 5C –Trisulfated Disaccharide Group (n = 15).</p

Liver histologic analysis.

<p>Liver histologic analysis.</p

Liver mitochondrial function.

<p>2A –RCR—Respiratory control rate * (TD vs SAL) p<0.05; # (CONTR vs SAL) p<0,05; 2B—ADP/O—ADP/Oxygen ratio * (SAL vs TD and SAL vs CONTR) p<0.05; 2C –S3—State 3 respiration * (SAL vs TD and SAL vs CONTR) p<0.05; 2D –S4—State 4 respiration NS: No Significance. CONTR—Control Group (n = 10); SAL—Saline Group; TD (n = 15)—Trisulfated Disaccharide Group (n = 15). Data are expressed as mean values.</p

Serum levels of inflammatory mediators.

<p>4A—TNF-α; 4B—IL-6; 4C—IL-10 * (TD vs SAL) p<0.05; # (CONTR vs SAL) p<0.05. CONTR—Control Group (n = 10); SAL—Saline Group (n = 15); TD—Trisulfated Disaccharide Group (n = 15). Data are expressed as mean values.</p

TD effect on thapsigargin-induced cytosolic calcium increase in liver cells (n = 3).

<p>Panel A—Images of 340/380 nm fluorescence ratio using a non-true color scale. Scale bars—50 μm; Time shown in white inside each frame; Panel B—Relative quantification of changes in calcium levels. Arrows indicate additions. THAP—4 μM thapsigargin; IONO—0.25 μM ionomycin; TD—30 μM trisulfated disaccharide.</p