31 research outputs found
AVALIAĂĂO DE MĂTODOS DE SELEĂĂO DE COMPETIDORES EM NĂVEL DE ĂRVORE INDIVIDUAL EM UMA FLORESTA ESTACIONAL SEMIDECIDUAL1
VariĂĄveis morfogĂȘnicas de milheto (Pennisetum americanum) mantido em duas alturas de pastejo
Contribuição dos componentes e composição quĂmica de pastagens em sistemas forrageiros constituĂdos por diferentes leguminosas
Land fauna composition of small mammals of a fragment of Atlantic Forest in the State of Sao Paulo, Brazil
Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens
Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens
In this work, we used the Minimum Inhibitory Concentration (MIC) technique to evaluate the antibacterial potential of the apitoxin produced by Apis mellifera bees against the causative agents of tooth decay. Apitoxin was assayed in natura and in the commercially available form. The antibacterial actions of the main components of this apitoxin, phospholipase A2, and melittin were also assessed, alone and in combination. The following bacteria were tested: Streptococcus salivarius, S. sobrinus, S. mutans, S. mitis, S. sanguinis, Lactobacillus casei, and Enterococcus faecalis. The MIC results obtained for the commercially available apitoxin and for the apitoxin in natura were close and lay between 20 and 40µg / mL, which indicated good antibacterial activity. Melittin was the most active component in apitoxin; it displayed very promising MIC values, from 4 to 40µg / mL. Phospholipase A2 presented MIC values higher than 400µg / mL. Association of mellitin with phospholipase A2 yielded MIC values ranging between 6 and 80µg / mL. Considering that tooth decay affects people's health, apitoxin and its component melittin have potential application against oral pathogens
Chemical Composition and Antibacterial Activity of the Essential Oil of Vitex agnus-castus L. (Lamiaceae)
ABSTRACT Abnormal multiplication of oral bacteria causes dental caries and dental plaque. These diseases continue to be major public health concerns worldwide, mainly in developing countries. In this study, the chemical composition and antimicrobial activity of the essential oil of Vitex agnus-castus leaves (VACâEO) collected in the North of Brazil against a representative panel of cariogenic bacteria were investigated. The antimicrobial activity of VAC-EO was evaluated in terms of its minimum inhibitory concentration (MIC) values by using the broth microdilution method in 96-well microplates. The chemical constituents of VAC-EO were identified by gas chromatography (GCâFID) and gas chromatographyâmass spectrometry (GCâMS). VACâEO displayed some activity against all the investigated oral pathogens; MIC values ranged from 15.6 to 200 ÎŒg/mL. VAC-EO had promising activity against Streptococcus mutans (MIC= 15.6 ÎŒg/mL), Lactobacillus casei (MIC= 15.6 ÎŒg/mL), and Streptococcus mitis (MIC= 31.2 ÎŒg/mL). The compounds 1,8-cineole (23.8%), (E)-ÎČ-farnesene (14.6%), (E)-caryophyllene (12.5%), sabinene (11.4%), and α-terpinyl acetate (7.7%) were the major chemical constituents of VACâEO. VAC-EO displays antimicrobial activity against cariogenic bacteria. The efficacy of VAC-EO against S. mutans is noteworthy and should be further investigated