Demographic and clinical characteristics of the study population.

<p>Demographic and clinical characteristics of the study population (n = 94). BMI: body mass index; ESR: erythrocyte sedimentation rate; HCT haematocrit; HDL-C: high density lipoprotein cholesterol; LDL-C: low density lipoprotein cholesterol; NLR neutrophil to lymphocyte ratio; PA: arterial pressure; PLR platelets to lymphocyte ratio; PT-INR pro-thrombin international normalised ratio; WBC White blood cells.</p><p>Demographic and clinical characteristics of the study population.</p

Recovered group vs unrecovered group.

<p>Clinical characteristics, blood and instrumental parameters of the recovered group and unrecovered group. ad: admission; AE: affected ear; BMI: body mass index; dis: discharge; ESR: erythrocyte sedimentation rate; HCT: haematocrit; HDL-C: high density lipoprotein cholesterol; LDL-C: low density lipoprotein cholesterol; NAE: non affected ear; NLR: neutrophil to lymphocyte ratio; PLR: platelets to lymphocyte ratio; PT-INR: pro-thrombin international normalised ratio; PTA: pure-tone average; WBC: White blood cells.</p><p>Recovered group vs unrecovered group.</p

Higher cholesterol levels correlate with lower recovery rates.

<p>Pearson’s correlation (P = 0.03; R = -0.2).</p

Gene expression profiling in GFD celiac patients.

<p>(A) Genes down-regulated in GFD celiac patients were profiled using microarrays, and clustered in large networks displaying a coordinate biological function. Data are shown in a heatmap with a matrix format of the genes differentially modulated within the specific network; single rows represent gene expression in a single patient (column). Colours: red, expression greater than the mean; black, expression equal to the mean; green, expression smaller than the mean. Lateral bars: fold changes among groups. (B) Proximity matrix of the RF algorithm: RF discriminated patients with celiac disease on GFD from control patients in 100% of cases (C-Index = 1). Legend: (black) controls; (white) celiac patients.</p

Characteristics of celiac patients.

<p>Characteristics of celiac patients.</p

Up-regulated genes in PBMCs as candidate biomarkers of celiac patients on GFD.

<p>(A) The ROC curves were characterized by specificity and sensitivity. (B) The cut-off values obtained with the ROC curves were able to discriminate GFD celiac patients from controls. Each patient is represented by a dot. Legend: (black) controls; (white) celiac patients.</p

Gene expression profiling in GFD celiac patients.

<p>(A) Genes up-regulated in GFD celiac patients were profiled using microarrays, and clustered in large networks displaying a coordinate biological function. Data are shown in a heatmap with a matrix format of the genes differentially modulated within the specific network; single rows represent gene expression in a single patient (column). Colours: red, expression greater than the mean; black, expression equal to the mean; green, expression smaller than the mean. Lateral bars: fold changes among groups. (B) Proximity matrix of the RF algorithm: RF discriminated patients with celiac disease on GFD from control patients in 100% of cases (C-Index = 1). Legend: (black) controls; (white) celiac patients.</p

RNA expression levels of TGF-β receptor I, TGF-β receptor II and TGF-β1 in different HCC cell lines evaluated by real-time PCR.

<p>Values are expressed as relative gene expression versus GAPDH and represent the mean ±SD of three independent experiments.</p

Comparison of the effect between LY2157299 and the fully humanized monoclonal antibody D10 on HCC cell morphology, actin cytoskeleton and E-cadherin expression.

<p>HepG2 cells were seeded onto glass coverslips at 3 x104 cells/ml in 24-well plates overnight and treated with control (A, G) or LY2157299 (E, K) or D10 (C, I) alone in serum-free medium. Then, cells were stimulated with TGF-β1 in the absence (B, H) or presence of LY2157299 (F, L) or D10 (D, J). LY2157299, D10 or TGF-β1 were added daily and after 72 hours cells were live-imaged under a phase contrast microscope. For immunofluorescence, cells were fixed with 4% paraformaldehyde, stained with TRITC-phalloidin and anti-E-cadherin antibody and examined under a NIKON eclipse E1000 fluorescence microscope. (M) Cell area was quantified using a combination of two digital image analysis softwares (ImageJ and Adobe Photoshop) and results were plotted as cell area (µm²) per random field.**P<0.01.</p

Immunofluorescence staining of TGF-β receptor II in frozen HCC tissues.

<p>Tissues from 30 patients with HCC were stained using D10, a fully humanized monoclonal antibody directed against TGF-β receptor II (red) and alpha smooth muscle actin with the monoclonal antibody clone 1A4 (green). In 11 out of 30 HCC tissues (36.6%) positivity for TGF-β receptor II was obtained; representative cases of TGF-β receptor II-positive (patient #22 and #29) or -negative (patients #8 and #19) tissues are shown.</p