Anxiety associated with palatable food withdrawal is reversed by the selective FAAH inhibitor PF-3845: A regional analysis of the contribution of endocannabinoid signaling machinery

Objective: Consumption of energy-dense palatable "comfort" food can alleviate stress and negative emotions, while abrupt withdrawal from a palatable diet can worsen these symptoms, causing difficulties with adherence to weight-loss diets. Currently, no pharmacological treatment is effective for obesity-related anxiety, so we investigated the endocannabinoid system (ECS), and specifically the fatty acid amide hydrolase (FAAH), as an interesting emerging target in this context because of its key role in the regulation of both energy homeostasis and emotional behavior. Methods: Rats were subjected to exposure and subsequent abstinence from a palatable cafeteria diet. During abstinence period, rats were treated with the selective FAAH inhibitor PF-3845 (10 mg/kg; intraperitoneal administration every other day). Results: Abstinent rats displayed an anxiogenic-like behavior and changes in the proteins of ECS signaling machinery in brain areas involved both in anxiety and food intake regulation. In particular, withdrawal caused a reduction of the expression of cannabinoid receptors in the nucleus accumbens and of enzymes diacylglycerol lipase alpha and monoacylglycerol lipase (MAGL) in the amygdala. Pharmacological inhibition of FAAH exerted an anxiolytic-like effect in abstinent animals and increased both MAGL expression in amygdala and CB2 expression in prefrontal cortex. Discussion: Overall, our results suggest that emotional disturbances associated with dieting are coupled with region-specific alterations in the cerebral expression of the ECS and that the enhancement of the endocannabinoid signaling by FAAH inhibition might represent a novel pharmacological strategy for the treatment of anxiety related to abstinence from palatable food.Funding for open access charge: Universidad de Málaga / CBUA European Regional DevelopmentFunds-European Union, Grant/Award Number:PI19/01577; Instituto de Salud Carlos III,Grant/Award Number: RETICS; Ministerio deCiencia e Innovaci on, Grant/Award Number:ERDF-EU RD16/0017/0001; Ministerodell'Università e della Ricerca, Grant/AwardNumber: 2012JTX3KL; PNRR-RomeTechnopole-FP

Phenotypic and genetic characterization of a family carrying two Xq21.1-21.3 interstitial deletions associated with syndromic hearing loss

Sensorineural hearing impairment is a common pathological manifestation in patients affected by X-linked intellectual disability. A few cases of interstitial deletions at Xq21 with several different phenotypic characteristics have been described, but to date, a complete molecular characterization of the deletions harboring disease-causing genes is still missing. Thus, the aim of this study is to realize a detailed clinical and molecular analysis of a family affected by syndromic X-linked hearing loss with intellectual disability

Small Renewable Energy Community: The Role of Energy and Environmental Indicators for Power Grid

The Clean Energy for all Europeans Package pushes for the diffusion of renewable energy communities, introducing their definition in the European legislative framework. Following this interest, this paper analyses the energy and environmental performance of a renewable energy community composed of two office buildings located in Naples (Italy). Each building has a rooftop photovoltaic plant and one office presents an electric vehicle. The heating and cooling demands of both offices are satisfied by two reversible air to water heat pumps. The offices are connected through an electric microgrid and they are in parallel with a power grid. Buildings and plants are modelled and simulated by means of TRNSYS 17 simulation software. The first analysis has concerned the comparison of the results achieved in renewable energy community configuration and from individual buildings in terms of quantity of electricity imported, exported from/to power grid and consumed on-site. The share of self-consumed photovoltaic electricity rises up to 79% when energy sharing is allowed. The second analysis has been carried out to evaluate the energy and environmental performance of a renewable energy community by means of fixed and hourly varying values for power grid efficiency and emission factors for electricity. The use of time-dependent indicators has led to a lower community primary energy demand and carbon dioxide emissions of 18% and 12%, respectively, in comparison with the scenario in which the fixed parameters have been adopted

The State of the Art of Smart Energy Communities: A Systematic Review of Strengths and Limits

The Smart Energy Community topic has attracted a lot of interest from policy, research centres, companies and private citizens since 2018, when in Europe the recast of the Renewable Energy Directive, and later in 2019 the Internal Electricity Market Directive, came into force to support the new role of users in energy systems. Following these directives, energy community experimentations, real projects and/or simulations and case studies have been developed and investigated in the literature. In this review paper, an investigation of recent literature about Smart Energy Communities in terms of common characteristics, fundamental scopes, and principal indexes used for their evaluation, has been realized by considering 111 scientific references, 78 of which have been published since 2018. The reference papers have been selected through the “Preferred Reporting Items for Systematic reviews and Meta-Analysis” methodology. In developing the review, significant barriers to Smart Energy Communities’ diffusion emerged. The main shortcomings concern citizens’ uncertainty about these new projects, due to their poor information and technical skills. These issues often hide energy, economic, environmental, and social benefits of Smart Energy Communities. Therefore, this study wants to be an opportunity for bringing to the attention of citizens Smart Energy Communities’ positive outcomes, especially from the social point of view, thus boosting their spreading and overcoming still existing criticalities

Energy, Environmental, and Economic Analyses of Geothermal Polygeneration System Using Dynamic Simulations

This paper presents a thermodynamic, economic, and environmental analysis of a renewable polygeneration system connected to a district heating and cooling network. The system, fed by geothermal energy, provides thermal energy for heating and cooling, and domestic hot water for a residential district located in the metropolitan city of Naples (South of Italy). The produced electricity is partly used for auxiliaries of the thermal district and partly sold to the power grid. A calibration control strategy was implemented by considering manufacturer data matching the appropriate operating temperature levels in each component. The cooling and thermal demands of the connected users were calculated using suitable building dynamic simulation models. An energy network dedicated to heating and cooling loads was designed and simulated by considering the variable ground temperature throughout the year, as well as the accurate heat transfer coefficients and pressure losses of the network pipes. The results were based on a 1-year dynamic simulation and were analyzed on a daily, monthly, and yearly basis. The performance was evaluated by means of the main economic and environmental aspects. Two parametric analyses were performed by varying geothermal well depth, to consider the uncertainty in the geofluid temperature as a function of the depth, and by varying the time of operation of the district heating and cooling network. Additionally, the economic analysis was performed by considering two different scenarios with and without feed-in tariffs. Based on the assumptions made, the system is economically feasible only if feed-in tariffs are considered: the minimum Simple Pay Back period is 7.00 years, corresponding to a Discounted Pay Back period of 8.84 years, and the maximum Net Present Value is 6.11 M€, corresponding to a Profit Index of 77.9% and a maximum Internal Rate of Return of 13.0%. The system allows avoiding exploitation of 27.2 GWh of primary energy yearly, corresponding to 5.49∙103 tons of CO2 avoided emissions. The increase of the time of the operation increases the economic profitability

Energy, Environmental, and Economic Analyses of Geothermal Polygeneration System Using Dynamic Simulations

This paper presents a thermodynamic, economic, and environmental analysis of a renewable polygeneration system connected to a district heating and cooling network. The system, fed by geothermal energy, provides thermal energy for heating and cooling, and domestic hot water for a residential district located in the metropolitan city of Naples (South of Italy). The produced electricity is partly used for auxiliaries of the thermal district and partly sold to the power grid. A calibration control strategy was implemented by considering manufacturer data matching the appropriate operating temperature levels in each component. The cooling and thermal demands of the connected users were calculated using suitable building dynamic simulation models. An energy network dedicated to heating and cooling loads was designed and simulated by considering the variable ground temperature throughout the year, as well as the accurate heat transfer coefficients and pressure losses of the network pipes. The results were based on a 1-year dynamic simulation and were analyzed on a daily, monthly, and yearly basis. The performance was evaluated by means of the main economic and environmental aspects. Two parametric analyses were performed by varying geothermal well depth, to consider the uncertainty in the geofluid temperature as a function of the depth, and by varying the time of operation of the district heating and cooling network. Additionally, the economic analysis was performed by considering two different scenarios with and without feed-in tariffs. Based on the assumptions made, the system is economically feasible only if feed-in tariffs are considered: the minimum Simple Pay Back period is 7.00 years, corresponding to a Discounted Pay Back period of 8.84 years, and the maximum Net Present Value is 6.11 M€, corresponding to a Profit Index of 77.9% and a maximum Internal Rate of Return of 13.0%. The system allows avoiding exploitation of 27.2 GWh of primary energy yearly, corresponding to 5.49∙103 tons of CO2 avoided emissions. The increase of the time of the operation increases the economic profitability

Biomass-Based Renewable Energy Community: Economic Analysis of a Real Case Study

Renewable energy communities are catalysts of social innovation, the citizens’ engagement in energy actions, and the exploitation of local resources. Thus, this paper defines a model for analyzing and optimally sizing energy systems serving renewable energy communities. Then, the proposed and replicable model was tailored to the economic feasibility analysis of a renewable energy community in the municipality of Tirano (Northern Italy). An energy audit was carried out to identify the electricity production and consumption within the perimeter of the primary substation and the thermal energy demand of the existing district heating network. The technical features of the energy conversion systems serving the renewable energy community were determined: an organic Rankine cycle biomass-based cogeneration plant, a mini-hydro plant, and a distributed photovoltaic system. Moreover, several different scenarios have been identified, in terms of cogeneration operating mode, photovoltaic penetration, and thermal energy economic value. The results show that, moving from 4.22 MW to 5.22 MW of photovoltaic peak power, the annual renewable electricity production increases by 10.1%. In particular, the simple pay back ranges between 4.90 and 4.98 years and the net present value between EUR 12.4 and 13.3 M for CHP operating at full power mode, considering that thermal energy available from the cogeneration unit is sold at EUR 49.2/MWh. These outcomes demonstrate the economic feasibility of wood-biomass-based renewable energy communities, which may help to enlarge the contribution of renewable technologies other than photovoltaic

Biomass-Based Renewable Energy Community: Economic Analysis of a Real Case Study

Renewable energy communities are catalysts of social innovation, the citizens’ engagement in energy actions, and the exploitation of local resources. Thus, this paper defines a model for analyzing and optimally sizing energy systems serving renewable energy communities. Then, the proposed and replicable model was tailored to the economic feasibility analysis of a renewable energy community in the municipality of Tirano (Northern Italy). An energy audit was carried out to identify the electricity production and consumption within the perimeter of the primary substation and the thermal energy demand of the existing district heating network. The technical features of the energy conversion systems serving the renewable energy community were determined: an organic Rankine cycle biomass-based cogeneration plant, a mini-hydro plant, and a distributed photovoltaic system. Moreover, several different scenarios have been identified, in terms of cogeneration operating mode, photovoltaic penetration, and thermal energy economic value. The results show that, moving from 4.22 MW to 5.22 MW of photovoltaic peak power, the annual renewable electricity production increases by 10.1%. In particular, the simple pay back ranges between 4.90 and 4.98 years and the net present value between EUR 12.4 and 13.3 M for CHP operating at full power mode, considering that thermal energy available from the cogeneration unit is sold at EUR 49.2/MWh. These outcomes demonstrate the economic feasibility of wood-biomass-based renewable energy communities, which may help to enlarge the contribution of renewable technologies other than photovoltaic

Phase-resolved measurements on a multi-slotted Synthetic Jets actuator

The free flow field of a multi-slotted array of Synthetic Jets (SJ) has been experimentally investigated in quiescent ambient at Reynolds and Strouhal numbers, Re = 128 and St = 0.11, respectively. The multi-slotted SJ actuator is composed of twelve rectangular slots with an aspect ratio of 15 (for a width of ~1 mm) and off-centered with respect to the cavity center. Experiments were conducted at three equally-spaced cross sectional planes inspecting two consecutive slots by means of phase-locked Stereo-Particle Image Velocimetry. At the slot exit, the statistical analysis reveals that the SJ exhibits an asymmetric spreading with respect to the geometrical axis of the slot, being tilted of about ~14.8° on the side where the cavity extends. This asymmetric pattern determines a small recirculation region slightly downstream of the slot exit. On the other hand, the flow field appears two-dimensional at the mid spanwise distance between the two slots. The phase-locked flow fields exhibit counter-rotating vortical patterns that convect downstream from the slot exit during the ejection phase. These vortices develop in longitudinal direction at 3.4 slot widths and then dissipate as they travel further downstream