Clinical significance of epithelial-to-mesenchymal transition in laryngeal carcinoma: Its role in the different subsites

Background: During epithelial-to-mesenchymal transition, cancer cells lose adhesion capacity gaining migratory properties. The role of the process on prognosis has been evaluated in 50 cases of laryngeal carcinoma. Methods: E-cadherin, N-cadherin, β-catenin, α-catenin, γ-catenin, caveolin-1, and vimentin immunohistochemical expression were evaluated using a double score based on staining intensity and cellular localization. Results: Cytoplasmic E-cadherin and α/γ catenin staining were associated with a decrease in survival, cytoplasmic β-catenin was associated with advanced stage, and N-cadherin and vimentin expression were associated with poor differentiation and tumor relapse. On the basis of cancer cells, epithelial or mesenchymal morphological and immunophenotypic similarity we identified 4 main subgroups correlated with a transition to a more undifferentiated phenotype, which have a different pattern of relapse and survival. Conclusion: The negative prognostic role of epithelial-to-mesenchymal transition has been confirmed and a predictive role in glottic tumors has been suggested, leading us to propose epithelial-to-mesenchymal transition as an additional adverse feature in laryngeal carcinoma

Resonant, broadband and highly efficient optical frequency conversion in semiconductor nanowire gratings at visible and UV wavelengths

Using a hydrodynamic approach we examine bulk- and surface-induced second and third harmonic generation from semiconductor nanowire gratings having a resonant nonlinearity in the absorption region. We demonstrate resonant, broadband and highly efficient optical frequency conversion: contrary to conventional wisdom, we show that harmonic generation can take full advantage of resonant nonlinearities in a spectral range where nonlinear optical coefficients are boosted well beyond what is achievable in the transparent, long-wavelength, non-resonant regime. Using femtosecond pulses with approximately 500 MW/cm2 peak power density, we predict third harmonic conversion efficiencies of approximately 1% in a silicon nanowire array, at nearly any desired UV or visible wavelength, including the range of negative dielectric constant. We also predict surface second harmonic conversion efficiencies of order 0.01%, depending on the electronic effective mass, bistable behavior of the signals as a result of a reshaped resonance, and the onset fifth order nonlinear effects. These remarkable findings, arising from the combined effects of nonlinear resonance dispersion, field localization, and phase-locking, could significantly extend the operational spectral bandwidth of silicon photonics, and strongly suggest that neither linear absorption nor skin depth should be motivating factors to exclude either semiconductors or metals from the list of useful or practical nonlinear materials in any spectral range.Comment: 12 pages, 4 figure

KMT2C modulates migration and invasion processes in osteosarcoma cell lines

In this study we investigated the role of KMT2C (a chromatin-modifying and remodelling protein) in osteosarcoma progression through cell migration and invasion assays in osteosarcoma primary and metastatic cell lines. Wound healing and transwell assays were used to detect changes of cell migration and matrigel assay was used to evaluate changes of cell invasion in primary and metastatic osteosarcoma cell lines after KMT2C siRNA transfection. We found that primary osteosarcoma cell lines showed the highest capacity of migration before mRNA KMT2C silencing and the highest capacity of invasion after mRNA KMT2C silencing; on the contrary, osteosarcoma metastatic cell line showed the highest capacity of migration after mRNA KMT2C silencing and the highest capacity of invasion before mRNA KMT2C silencing. Our study supports data in favour of selective enhancer changes, KMT2C-mediated, in metastatic osteosarcoma probably due to the different microenvironment between primary and metastatic sites

Scaling of the Critical Function for the Standard Map: Some Numerical Results

The behavior of the critical function for the breakdown of the homotopically non-trivial invariant (KAM) curves for the standard map, as the rotation number tends to a rational number, is investigated using a version of Greene's residue criterion. The results are compared to the analogous ones for the radius of convergence of the Lindstedt series, in which case rigorous theorems have been proved. The conjectured interpolation of the critical function in terms of the Bryuno function is discussed.Comment: 26 pages, 3 figures, 13 table

Modelling and nanofabrication of chiral dielectric metasurfaces

Polarization control through all-dielectric metasurfaces holds great potential in different fields, such as telecommunications, biochemistry and holography. Asymmetric chiral metasurfaces supporting quasi-bound states in the continuum may prove very useful for controlling and manipulating the polarization state of light. A crucial quantity for characterizing the optical chirality is the circular dichroism (CD). In this work we analyse how the CD and quality factor of the optical mode can be strongly influenced by a nanofabrication error. Modelling the nanofabrication uncertainties on the gaps of the chiral metasurface, the imperfections of the etchings process or the modification of the asymmetry factor, we found that the proper engineering of the gap between the nanostructures of the unit cell is the most important parameter to achieve a high-quality factor and enhanced optical dichroism. An optimization of the nanofabrication processes, such as dose factor, dwell time and plasma etching demonstrates that, for a writing field of 100 & mu;m2, it is possible to obtain morphologically precise chiral metasurfaces, with fabrication uncertainties lower than those that would limit Q factor and chirality property

Recent expansion of dengue virus serotype 3 in West Africa

Due to non-existing or limited surveillance in Africa, little is known about the epidemiology of dengue illness in the continent. Serological and virological data obtained from returning European travellers is a key complement to this often flawed information. In the past years, dengue 3 virus has emerged in West Africa and has been detected in travellers returning to Europe. The first dengue epidemic in Cape Verde with more than 17,000 cases from September to December 2009 demonstrated that dengue virus is still expanding worldwide to new territories.S

Structures for Knowledge Co-creation Between Organisations and the Public

A definitive version of this conference paper is available in the ACM Digital Library http://dx.doi.org/10.1145/2556420.2558854© ACM 2014Social computing technologies are emerging to support innovative new relationships between organisations and the public. Inspired by concepts such as collective intelligence and crowdsourcing, diverse organisations are exploring new ways to increase engagement with the public, collect localised knowledge, or leverage human cognition and creativity. The COP2014 workshop will assimilate the craft understanding and experimentation underpinning innovations in relationships between public and professional realms. We aim to provide an opportunity for discussion and to build understanding of the principles of making these sociotechnical systems work, and discuss possible designs and opportunities that may be valuable and remain underexplored. As a tangible outcome, our main goal will be to co-author a paper discussing our positions on current systems and future visions, for submission to Communications of the ACM. We welcome participation from interested organisations, alongside those conducting research in this area. Topics of interest include: How can systems be designed for participation that is beneficial to individuals and organisations? How do we draw boundaries between ‘official’ and externally-generated knowledge? How can professional activities connect with the just-in-time, emergent nature of amateur interactions?RCUK Horizon Hu