Cultivating compassion through analysis of online patient narratives

Photos of male genitalia of Cx. univittatus from South Africa and Portuguese specimens. (PDF 3361 kb

Corine Land Cover 2006 classes.

<p>Corine Land Cover 2006 classes.</p

Maximum and minimum values of <i>An</i>. <i>atroparvus’</i> abundance in mainland Portugal.

<p>Maximum and minimum values of <i>An</i>. <i>atroparvus’</i> abundance in mainland Portugal.</p

Concordance between former malaria areas and malariogenic potential >1 in scenario A0, A1, and A2.

<p>Concordance between former malaria areas and malariogenic potential >1 in scenario A0, A1, and A2.</p

% maximum <i>kappa</i> of the five predictive models.

<p>% maximum <i>kappa</i> of the five predictive models.</p

Imported malaria cases in Portugal, from 2010 to 2013 [55].

<p>Imported malaria cases in Portugal, from 2010 to 2013 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164788#pone.0164788.ref055" target="_blank">55</a>].</p

<i>An</i>. <i>atroparvus’</i> abundance.

<p><i>An</i>. <i>atroparvus’</i> abundance.</p

Influence of LPS in cell viability.

<p>RAW 264.7 macrophages were exposed to 1 µg/mL of LPS, during 18 h and cell viability were assessed by LDH and MTT assays. Results are expressed in percentage of control (mean ± SEM of five independent experiments, performed in duplicate). *P<0.05, ***P<0.001.</p

Superoxide radical quantification.

<p>Superoxide radical produced by RAW 264.7 macrophages, after pre-treatment (1 h) with diosquinone (1.5 µM) and dexamethasone (50 µM), in the presence and in the absence of 1 µg/mL of LPS (18 h). Results are expressed in percentage of control (mean ± SEM of four independent experiments, performed in duplicate).</p

Influence of plumbagin in cell viability and in NO production.

<p>After pre-exposure with plumbagin and stimulation with LPS, RAW 264.7 macrophages viability was assessed by LDH and MTT assays and NO production was quantified. Results are expressed as percentage of control (mean ± SEM of four independent experiments, performed in duplicate).**P<0.01, ***P<0.001.</p