Notification rates (/10) per year, for the whole population (FM) and by gender

<p><b>Copyright information:</b></p><p>Taken from "Tuberculosis incidence in Portugal: spatiotemporal clustering"</p><p>http://www.ij-healthgeographics.com/content/6/1/30</p><p>International Journal of Health Geographics 2007;6():30-30.</p><p>Published online 11 Jul 2007</p><p>PMCID:PMC1965471.</p><p></p

Distribution of ten most frequently AP by gender, 2004–2013.

<p>Distribution of ten most frequently AP by gender, 2004–2013.</p

Characterization of discharges by ADE, ADR & AP, considering year, age, gender, length of stay and death, 2004–2013.

<p>Characterization of discharges by ADE, ADR & AP, considering year, age, gender, length of stay and death, 2004–2013.</p

C3G and 5-ASA inhibit the production of pro-inflammatory mediators induced by cytokines in HT-29 cells.

<p>Cells were pre-incubated with 25 µM C3G or 500 µM 5-ASA or both (25 µM C3G plus 500 µM 5-ASA) and then treated with cytokines for a certain period of time. NO (A), PGE₂ (B) and IL-8 (C) production by cells were measured as described in “Materials and Methods”. Values are mean ± SEM of at least three different experiments, in duplicate. ^***<i>P</i><0.001 vs Control, ^##<i>P</i><0.01, ^###<i>P</i><0.001 vs Cytokines. ^$$<i>P</i><0.01, ^$$$<i>P</i><0.001 vs 5-ASA plus Cytokine mixture.</p

C3G and 5-ASA reduce the levels of cytokine-induced STAT1 activation in HT-29 cells.

<p>Cells were pre-incubated with 25 µM C3G or 500 µM 5-ASA or both and then treated with a combination of cytokines for 30 minutes. STAT1 phosphorylation was evaluated in nuclear extracts by Western blotting, as described in “Material and Methods”. Values are mean ± SEM of at least three different experiments, in duplicate. ^***<i>P</i><0.001 vs Control, ^#<i>P</i><0.05,^##<i>P</i><0.01 vs Cytokines.</p

Effects of C3G and 5-ASA on the cell viability of HT-29 cells.

<p>HT-29 cells were incubated for 24 hours with different concentrations of C3G (12.5 to 50 µM), 5-ASA (500 µM) and a combination of 25 µM C3G and 500 µM 5-ASA. Cell viability was assessed by MTT reduction and determined as percentage of control cells (without compounds). Values are mean ± SEM of at least three different experiments, in duplicate.</p

C3G and 5-ASA do not suppress the activation of NF-kB-p65 in HT-29 cells.

<p>Cells were pre-incubated with 25 µM C3G or 500 µM 5-ASA or both and then treated with a combination of cytokines for 30 minutes. NF-kB activation was evaluated in nuclear extracts by a DNA-binding activity assay. Values are mean ± SEM of at least three different experiments, in duplicate. ^***<i>P</i><0.001 vs Control.</p

C3G inhibits cytokines-induced up-expression of iNOS and COX-2, like 5-ASA, in HT-29 cells.

<p>Cells were pre-incubated with 25 µM C3G or 500 µM 5-ASA or both (25 µM C3G plus 500 µM 5-ASA) and then treated with a combination of cytokines. iNOS (A) and COX-2 (B) expressions were evaluated after 24 hours or 16 hours, respectively, in total extracts by Western blotting, as described in “Materials and Methods”, and expressed as percentage of control. Values are mean ± SEM of at least three different experiments, in duplicate. ^***<i>P</i><0.001 vs Control, ^#<i>P</i><0.05,^##<i>P</i><0.01, ^###<i>P</i><0.001 vs Cytokines.</p

Failure of C3G and 5-ASA to inhibit cytokine-induced phosphorylation of p38 MAPK in HT-29 cells.

<p>Cells were pre-incubated with 25 µM C3G or 500 µM 5-ASA or both and then treated with a combination of cytokines for 30 minutes. Phosphorylation of p38 MAPK was evaluated in total extracts by Western blotting, as described in “Material and Methods”. Values are mean ± SEM of at least three different experiments, in duplicate. ^***<i>P</i><0.001 vs Control.</p

Video_2_Methodologies for Patellid Limpets’ Aquaculture: From Broodstock Management to Juveniles.mp4

The production of cultured limpets is a recent research field contributing to aquaculture diversification, focusing on low trophic species while reducing the carbon footprint. Limpets are gastropods that colonize rocky substrates and are mostly present on tidal and subtidal shores. This animal group is in high commercial demand and is endangered in several regions. The aquaculture production of limpets has been traditionally challenging. The most successful reproduction method has been gonadal dissection, as artificial spawning induction has shown limited success to date. Moreover, methods for larval culture, settlement, and juvenile growth have been poorly developed and remain largely unknown. In recent years, advances in this field have led to the optimization of methods to enhance larval production, larval culture, settlement induction of competent larvae, and management of post-larvae and juveniles. The present manuscript reviews these advances, obtained within the framework of AQUAINVERT project, focusing on broodstock management, gametes release, larval production, larviculture, settlement, and grow-out of post-larvae, and providing an update on the actual state of the art in limpets’ aquaculture.</p