9 research outputs found
List of secreted proteins that are common in both <i>T</i>.<i>cruzi</i> strains without of multigene proteins.
<p>List of secreted proteins that are common in both <i>T</i>.<i>cruzi</i> strains without of multigene proteins.</p
Analyse of trans-sialidase (TcS) proteins found in secretome of 2 strains.
<p>Classification of TcS proteins for each strain into 8 group previously described [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0185504#pone.0185504.ref022" target="_blank">22</a>]. Groups IV, V and VI are less than I, II, III groups VII and VIII for two strains. On the x-axis, the number of group is indicated. The Y-axis shows the percentage of each TcS identified in our analyses.</p
List of the <i>Candida</i> species and strains used in the study.
<p>ATCC, American Type Culture Collection, Manassas, VA, USA. PS, Pitié-Salpétrière Hospital, AP-HP, Paris, France. *used as reference strain for building database in statistical analysis.</p
Functional categories classification of <i>T</i>. <i>cruzi</i> proteins from CL Brener and VD strains.
<p>Proteins were classified into 15 functional categories using literature and UniprotKB annotation. Y-axis, categories are indicated. X-axis shows the percentage of each category for each strain.</p
Overlap between secretomes of two different <i>T</i>. <i>cruzi</i> strains DTU Tc VI.
<p>A total of 591 proteins of <i>T</i>. <i>cruzi</i> were identified. We note that 78 proteins are specific to the CL Brener strain whereas 151 proteins are specific to VD strain. However, 363 proteins are common to both strains.</p
Mean correlation coefficients of tested strains and clinical sample of patient against the seven-classes database.
<p>*denotes results obtained from positive blood cultures that had been stored for 24 hours at 4°C prior to SDS extraction. ** See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008862#s2" target="_blank">Methods</a> paragraph. The best score is presented in bold.</p
Mass spectra from isolates belonging to either <i>L. (V) braziliensis, L. (V) guyanensis ((V)</i> stands for <i>Viannia</i> subgenus), <i>L. (L) major, L. (L) infantum</i> ((L) stands for <i>Leishmania</i> subgenus).
<p>The two pairs of peaks discriminating the <i>Viannia</i> subgenus from the <i>Leishmania</i> subgenus are labeled in green and blue, respectively and indicated by vertical dotted lines. The 11120+/−(7) peak that identifies the <i>Viannia</i> subgenus is shown in insert squares at the right side of the figure to improve readability. Peaks differentiating species complexes are labeled with their corresponding molecular weights in colored squares. The software automatically provides the molecular weights for all peaks above signal background (grey labels). Peaks that identify species in each subgenus are shown in Black. A few peaks above background were not labeled on the figure to improve readability, the complete spectra are provided as supplementary <b><a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002841#pntd.0002841.s001" target="_blank">figure S1</a></b>.</p
Cluster analysis of MALDI-TOF MS 184 spectra from 46 <i>Leishmania</i> isolates (A) with distances displayed in relative units [19], and algorithm for a computer-independent interpretation of MALDI-TOF MS (B) based on presence/absence of peaks as displayed on Table 1.
<p>Cluster analysis of MALDI-TOF MS 184 spectra from 46 <i>Leishmania</i> isolates (A) with distances displayed in relative units <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002841#pntd.0002841-MarinachPatrice2" target="_blank">[19]</a>, and algorithm for a computer-independent interpretation of MALDI-TOF MS (B) based on presence/absence of peaks as displayed on <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002841#pntd-0002841-t001" target="_blank">Table 1</a>.</p
Alterations in the mass spectra (virtual gel) of blood cultures injected with different yeast strains.
<p>The x-axis represents Da value, For each strain 4 spectra (numbered on the left Y-axis) from two biological replicates deposited twice on the spectrometer plate are presented. A grey colour scale for peak intensity with arbitrary units is provided on the right y-axis. Each spectrum was automatically collected in the positive ion mode as an average of 700 laser shots. Mass range 3,000–20,000 Da was selected with a signal/noise ratio >3 (S/N) and resolution better than 600 (Flexcontrol software 2.4; Bruker-Daltonics). The control sample was from a yeast-free blood culture.</p