Gelsolin expression and distribution around developing <i>P. berghei</i>.

<p>(<b>A</b>) Microarray data of GSN expression levels in <i>P. berghei</i>- infected and non infected Hepa1-6 cells at different time points of infection, from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029408#pone.0029408-Albuquerque1" target="_blank">[18]</a> (<b>B</b>) qRT-PCR data of GSN expression in <i>P. berghei</i> infected and non-infected Huh7 cells at 12 hours p.i.. (<b>C</b>) Huh7 cells showing an accumulation of GSN around GFP-Pb in a pattern similar to an actin cloud and tail-like actin cloud (red and grey: anti-GSN antibody, green: GFP-Pb; blue: nuclei). Scale bars represent 10 µm.</p

Gelsolin is important for actin reorganization around developing <i>P. berghei</i> and parasite disappearance.

<p>(<b>A</b>) GSN mRNA (top) and protein (bottom) expression after knockdown with shRNA. GSN and α-tubulin bands corresponds to 93 kDa and 55 kDa molecular weights, respectively. (<b>B</b>) Phalloidin staining of Huh7 cells transduced with Scramble and GSN shRNA (grey: F-actin). (<b>C</b>) Effect of GSN knockdown on the percentage of <i>P. berghei</i> parasites associated with hepatocyte actin reorganization between 10 and 16 hours p.i.. Numbers of parasites associated with actin reorganization/total numbers of parasites analysed are indicated above the bars. Error bars represent Standard Errors * p<0.05 ** p<0.01; scale bars represent 10 µm.</p

Hepatocyte cytoskeleton reorganization around developing P. berghei <i>in vitro</i> and <i>in vivo</i>.

<p>(<b>A</b>) Actin but not tubulin is reorganized in Huh7 cell lines infected with GFP-Pb parasites (red and grey: mCherry::α-tubulin or mCherry::β-actin; green: GFP-Pb). Plot profiles represent the pixel intensity (gray values) of the regions indicated in the pictures (lines); <b>o</b> represents the parasite. Pie plots represent the percentages and absolute numbers of parasites associated (▪) or not (□) with host cytoskeleton reorganization. (<b>B</b>) Distribution of the percentage of <i>P. berghei</i> parasites associated with host cell actin reorganization in different infection periods. Numbers of parasites associated with actin reorganization/total numbers of parasites analysed are indicated above the bars. (<b>C</b>) Hepatocyte F-actin around <i>P. berghei</i> EEFs at 24 hours p.i. in BALB/c mice livers (red and grey: Phalloidin AlexaFluor 594; green: GFP-Pb; blue: nuclei). The pie plot represents the percentage and absolute numbers of parasites associated (▪) or not (□) with host actin reorganization. Plot profile represents the pixel intensity (gray values) along the white line; <b>o</b> represents the parasite. Error bars represent Standard Errors ** p<0.01, scale bars represent 10 µm.</p

Hepatocyte cytoskeleton reorganization depends on the presence of <i>P. berghei</i>.

<p>Percentage of parasites (<i>P. berghei</i> and <i>T. gondii</i>) and beads associated with hepatocyte actin reorganization between 10 and 16 hours p.i. or bead internalization. Numbers of parasites or beads associated with actin reorganization/total numbers of parasites or beads analysed are indicated above the bars. Error bars represent Standard Errors * p<0.05 *** p≤0.001.</p