8 research outputs found
Skilled reaching test.
<p>Skilled reaching test at different times of recovery after PT stroke for animals housed in standard (STD) and enriched environment (EE). (A) The total number of pasta pieces reached during 20 minutes prior to PT (pre) and at different time points after PT. (#: p<0.05 when compared to the respective group before stroke (pre). (B) Map of the reaching area presenting the number of animals (as indicated by colors) that reached the pasta pieces (n = 7 for each housing condition).</p
Experimental design.
<p>Rats were trained, and baseline values set for the different functional tests. Rats were then subjected to PT and the functional deficits assessed 2 days later. Subsequently, rats were placed either in standard (STD) or enriched environment (EE). Functional tests were regularly performed during 9 weeks following the onset of the stroke (the time of the different tests are represented by color dots). At 9 weeks, rats were perfusion fixed and brains were analyzed by immunohistochemistry.</p
Neuroscore.
<p>The normal neuroscore before stroke is 28. Values at different times after PT in rats subsequently housed in standard (STD) and enriched environment (EE) are presented in box plot: median; 1<sup>st</sup> quartile to 3<sup>rd</sup> quartile, minimum and maximum value. (n = 7 for each housing condition) (*: p<0.05 when STD compared to EE; #: p<0.05 when STD and EE where compared to the respective values before stroke).</p
Effect of stroke on gait.
<p>Presentation of gait parameters and the changes induced by PT. (A) Principle of the Catwalk. Rats run on a glass plate illuminated from the side. When the paws press the plate, light is diffracted and captured by a camera below the glass plate. The calculation of the gait parameters require the following steps: 1) the runs are recorded, 2) the light spots are associated with their respective paws, 3) the sequence of paw contact with the glass plate over time is determined and 4) the different gait parameters are calculated. (B) Stroke induces changes in different gait parameters. Here are summarized the general effect of stroke on gait two days after stroke. (C) Stroke also induces an asymmetry between left and right side in some gait parameters. The colored boxes represent time fractions when the paws are in contact with the surface of the glass plate. The black lines represent the values before stroke and the red lines the direction as well as increase or decrease of the values at 2 days after stroke (LH: left hind limb, RH: right hind limb). For details see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093121#pone.0093121.s003" target="_blank">Table S3</a>.</p
Environmental stimulation decreases the number of AB1031<sup>+</sup> PV/GABA neurons in the rat cerebral cortex after PT.
<p>(A) Confocal images of parvalbumin expressing cells (green) enwrapped by AB1031<sup>+</sup> PNNs (red) in the somatosensory cortex of a representative STD animal, scale bar 20 μm. (B and C) Representative bright-field micrographs of AB1031<sup>+</sup> PNNs in the rat cerebral cortex ipsilateral to the lesion, scale bar 100 μm; (B1 and C1) higher magnification, scale bar 20 μm. AB1031 immunoreactivity in (B) STD and (C) EE conditions. Quantification of cortical neurons bearing AB1031<sup>+</sup> PNNs in the peri-infarct cortex (D), the corresponding area contralateral to the lesion (E), the ipsilateral somatosensory cortex (F) and the contralateral somatosensory cortex (G). (n = 7 for each housing condition; 9 weeks after stroke; D with *p = 0.04; E with p = 0.3613; F with *p = 0.007; G with p = 0.0963; STD: standard environment, EE: enriched environment, PI: peri-infarct cortex, SS: somatosensory cortex, IPSI: ipsilateral, CONTRA: contralateral).</p
Enriched housing decreases the number of Cat-315<sup>+</sup> PV/GABA neurons in the rat cerebral cortex after PT.
<p>(A) Confocal images of a parvalbumin (PV) expressing cell (green) enwrapped by a Cat-315<sup>+</sup> PNN (red) in the somatosensory cortex of a representative STD animal; Z-stack demonstrates close proximity of the Cat-315 aggrecan antibody and PV, scale bar 20 μm. (B and C) Representative bright-field micrographs of Cat-315<sup>+</sup> PNNs in the rat cerebral cortex ipsilateral to the lesion, scale bar 100 μm; (B1 and C1) higher magnification, scale bar 20 μm. Cat-315 immunoreactivity denotes a critical difference between (B) STD and (C) EE conditions. Quantification of cortical neurons bearing Cat-315<sup>+</sup> PNNs in the peri-infarct cortex (D), the corresponding area contralateral to the lesion (E), the ipsilateral somatosensory cortex (F) and the contralateral somatosensory cortex (G). (n = 7 for each housing condition; 9 weeks after stroke; D with *p = 0.04; E with p = 0.06; F with *p = 0.0007; G with p = 0.1; STD: standard environment, EE: enriched environment, PI: peri-infarct cortex, SS: somatosensory cortex, IPSI: ipsilateral, CONTRA: contralateral).</p
Infarct Volume.
<p>Infarct volume was measured at 9 weeks after PT, and estimated by integration of the lesioned area on brain slices taken every 1-caudal brain axis. NeuN staining was performed to discriminate between intact and infarcted tissue. (A) Successive coronal slides of a representative brain infarct with their respective distances from the bregma for the STD housed animals, and (B) EE housed animals. (C) Infarct volume expressed as percentage of the hemisphere. Values are presented in box plots: median; 1<sup>st</sup> quartile to 3<sup>rd</sup> quartile; minimum and maximum value. (n = 7 for each housing condition; STD: standard environment, EE: enriched environment).</p
Paw placement test.
<p>Limb placing ability of rats subjected to PT under conditions that prevented visual and whisker/chin stimulation during testing. (A) Fore paw placement, and (B) hind paw placement. The normal score before stroke for rats is 1. Each dot corresponds to a rat value and the black line corresponds to the median value for the group. (n = 7 for each housing condition) (*: p<0.05 when STD compared to EE; #: p<0.05 when STD and EE where compared to the respective values before stroke; STD: standard environment, EE: enriched environment).</p