161 research outputs found
Nitrogenase Cofactor Maturase NifB Isolated from Transgenic Rice is Active in FeMo-co Synthesis
The engineering of nitrogen fixation in plants
requires assembly of an active prokaryotic nitrogenase complex,
which is yet to be achieved. Nitrogenase biogenesis relies on NifB,
which catalyzes the formation of the [8Fe−9S−C] metal cluster
NifB-co. This is the first committed step in the biosynthesis of the
iron−molybdenum cofactor (FeMo-co) found at the nitrogenase
active site. The production of NifB in plants is challenging because
this protein is often insoluble in eukaryotic cells, and its [Fe−S]
clusters are extremely unstable and sensitive to O2. As a first step to
address this challenge, we generated transgenic rice plants
expressing NifB from the Archaea Methanocaldococcus infernus
and Methanothermobacter thermautotrophicus. The recombinant proteins were targeted to the mitochondria to limit exposure to O2
and to have access to essential [4Fe−4S] clusters required for NifB-co biosynthesis. M. infernus and M. thermautotrophicus NifB
accumulated as soluble proteins in planta, and the purified proteins were functional in the in vitro FeMo-co synthesis assay. We thus
report NifB protein expression and purification from an engineered staple crop, representing a first step in the biosynthesis of a
functional NifDK complex, as required for independent biological nitrogen fixation in cereals
The Biosynthesis of Non-Endogenous Apocarotenoids in Transgenic Nicotiana glauca
Crocins are high-value compounds with industrial and food applications. Saffron is currently the main source of these soluble pigments, but its high market price hinders its use by sectors, such as pharmaceutics. Enzymes involved in the production of these compounds have been identified in saffron, Buddleja, and gardenia. In this study, the enzyme from Buddleja, BdCCD4.1, was constitutively expressed in Nicotiana glauca, a tobacco species with carotenoid-pigmented petals. The transgenic lines produced significant levels of crocins in their leaves and petals. However, the accumulation of crocins was, in general, higher in the leaves than in the petals, reaching almost 302 µg/g DW. The production of crocins was associated with decreased levels of endogenous carotenoids, mainly β-carotene. The stability of crocins in leaf and petal tissues was evaluated after three years of storage, showing an average reduction of 58.06 ± 2.20% in the petals, and 78.37 ± 5.08% in the leaves. This study illustrates the use of BdCCD4.1 as an effective tool for crocin production in N. glauca and how the tissue has an important impact on the stability of produced high-value metabolites during storage.This work was supported by grants BIO2016-77000-R from the Spanish Ministerio de Ciencia; Innovación y Universidades and SBPLY/17/180501/000234 from the Junta de Comunidades de Castilla-La Mancha (co-financed European Union FEDER funds); the National Natural Science Foundation of China (31870278); and the Spanish Ministry of Economy and Competitiveness (MINECO), Spain (RTI2018–097613-B-I00; PGC2018–097655-B-I00). C.Z. and L.G.G. are participants of the European COST action CA15136 (EUROCAROTEN) and Programa Estatal de Investigación Científica y Técnica de excelencia, Spain (BIO2015–71703-REDT and BIO2017–90877-REDT)
Functional expression of the nitrogenase Fe protein in transgenic rice
Engineering cereals to express functional nitrogenase is a long-term goal of plant biotechnology and would permit partial or total replacement of synthetic N fertilizers by metabolization of atmospheric N2. Developing this technology is hindered by the genetic and biochemical complexity of nitrogenase biosynthesis. Nitrogenase and many of the accessory proteins involved in its assembly and function are O2 sensitive and only sparingly soluble in non-native hosts. We generated transgenic rice plants expressing the nitrogenase structural component, Fe protein (NifH), which carries a [4Fe-4S] cluster in its active form. NifH from Hydrogenobacter thermophilus was targeted to mitochondria together with the putative peptidyl prolyl cis‐trans isomerase NifM from Azotobacter vinelandii to assist in NifH polypeptide folding. The isolated NifH was partially active in electron transfer to the MoFe protein nitrogenase component (NifDK) and in the biosynthesis of the nitrogenase iron-molybdenum cofactor (FeMo-co), two fundamental roles for NifH in N2 fixation. NifH functionality was, however, limited by poor [4Fe-4S] cluster occupancy, highlighting the importance of in vivo [Fe-S] cluster insertion and stability to achieve biological N2 fixation in planta. Nevertheless, the expression and activity of a nitrogenase component in rice plants represents the first major step to engineer functional nitrogenase in cereal crops
Metabolic engineering in plants for food security
Abstract Over 800 million people around the world do not have enough to eat, and many more endure monotonous diets that fall short of even basic nutritional requirements. Food and nutritional insecurity are perhaps the most pressing and intractable social issues now faced by the world's governments and populations, and they can only be addressed by a combination of measures that aim to improve health, wealth and agricultural productivity in a sustainable fashion. Genetically modified plants represent one of these measures, and many different strategies can be used to improve the yield, nutritional properties and agronomic performance of our crops. In particular, the metabolic engineering of crop plants is a versatile approach for enhancing the production of beneficial, small metabolites. Such molecules can provide nutritional benefits, protect plants against biotic and abiotic stresses and improve the ripening and storage properties of harvested products. In this review, we focus on two examples -vitamin biosynthesis and polyamine metabolism -to show how advances in plant metabolic engineering can offer hope to the world's poor and hungry
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