Analyzing the Professional Concern Levels of Physical Education and Sports Teacher Students’ in Terms of Different Variables

AbstractPurpose: In modern times, it's generally accepted that students experience professional concern in finding job after graduating from the university. The purpose of this study is to analyze anxiety levels of physical education and sport teachers’ in terms of different variables such as sex, class level, age, the sports type and level of family income. Method: 93 students (32 female and 61 male) took part in the study. As the data collection tool, “The Professional concern scale for teacher candidates”, developed by Cabi and Yalçınalp (2013) and composed of eight sub-dimensions was used. For the data analysis; descriptive statistics, the Mann–Whitney U test, and Kruskal-Wallis test were applied. Findings: At the results of analysis, there was statistically significant difference (p<0,05) between two groups at the sub-dimension, coherence-centric, of the Professional concern scale for teacher candidates according to the sex variable while a statistically significant difference was not found in terms of sex at others sub-dimensions of the scale. There was no statistically significant difference in terms of the variables such as the class level, the sports type and level of family income at any sub-dimensions of the scale. Result: In this study, the Professional concern levels of physical education and sport teachers’ in terms of different variables such as sex, class level, age, the sports conducted and level of family income were analyzed. While there was a statistically significant difference at the sub-dimension, coherence-centric, it was seen that there was no statistically significant difference at others sub-dimensions in term of any variables. That there wasn’t much difference at concern level of the students may result from the education levels of students, closed to each other

15-Lipoxygenase-1 re-expression in colorectal cancer alters endothelial cell features through enhanced expression of TSP-1 and ICAM-1

15-lipoxygenase-1 (15-LOX-1) oxygenates linoleic acid to 13(S)-hydroxyoctadecadienoic acid (RODE). The enzyme is widely suppressed in different cancers and its re-expression has tumor suppressive effects. 15-LOX-1 has been shown to inhibit neoangiogenesis in colorectal cancer (CRC); in the present study we confirm this phenomenon and describe the mechanistic basis. We show that re-expression of 15-LOX-1 in CRC cell lines resulted in decreased transcriptional activity of HIF1 alpha and reduced the expression and secretion of VEGF in both normoxic and hypoxic conditions. Conditioned medium (CM) was obtained from CRC or prostate cancer cell lines re-expressing 15-LOX-1 (15-LOX-1CM). 15-LOX-1CM treated aortic rings from 6-week old C57BL/6 mice showed significantly less vessel sprouting and more organized structure of vascular network. Human umbilical vein endothelial cells (HUVECs) incubated with 15-LOX-1CM showed reduced motility, enhanced expression of intercellular cell adhesion molecule (ICAM-1) and reduced tube formation but no change in proliferation or cell cycle distribution. HUVECs incubated with 13(S)-HODE partially phenocopied the effects of 15-LOX-1CM, showed reduced motility and enhanced expression of ICAM-1, but did not reduce tube formation, implying the importance of additional factors. Therefore, a Proteome Profiler Angiogenesis Array was carried out, which showed that Thrombospondin-1 (TSP-1), a matrix glycoprotein known to strongly inhibit neovascularization, was expressed significantly more in HUVECs incubated with 15-LOX-1CM. TSP-1 blockage in HUVECs reduced the expression of ICAM-1 and enhanced cell motility, thereby providing a mechanism for reduced angiogenesis. The anti-angiogenic effects of 15-LOX-1 through enhanced expressions of ICAM-1 and TSP-1 are novel findings and should be explored further to develop therapeutic options

Characterization of greater middle eastern genetic variation for enhanced disease gene discovery

The Greater Middle East (GME) has been a central hub of human migration and population admixture. The tradition of consanguinity, variably practiced in the Persian Gulf region, North Africa, and Central Asia1-3, has resulted in an elevated burden of recessive disease4. Here we generated a whole-exome GME variome from 1,111 unrelated subjects. We detected substantial diversity and admixture in continental and subregional populations, corresponding to several ancient founder populations with little evidence of bottlenecks. Measured consanguinity rates were an order of magnitude above those in other sampled populations, and the GME population exhibited an increased burden of runs of homozygosity (ROHs) but showed no evidence for reduced burden of deleterious variation due to classically theorized ‘genetic purging’. Applying this database to unsolved recessive conditions in the GME population reduced the number of potential disease-causing variants by four- to sevenfold. These results show variegated genetic architecture in GME populations and support future human genetic discoveries in Mendelian and population genetics

Liquid Biopsy for Promising Non-invasive Diagnostic Biomarkers in Parasitic Infections

Background Liquid biopsy refers to the sampling and molecular analysis of body fluids such as blood, saliva, and urine in contrast to conventional tissue biopsies. Liquid biopsy approach can offer powerful non-invasive biomarkers (circulating markers) for diagnosis and monitoring treatment response of a variety of diseases, including parasitic infections. Methods In this review, we concentrate on cell-free DNA (cfDNA), microRNA (miRNA), and exosomes in the published literature. Results Considering the high prevalence and severity of parasitic infections worldwide, circulating biomarkers can provide a new insight into the diagnosis and prognosis of parasites in the near future. Moreover, identifying and characterizing parasite- or host-derived circulating markers are important for a better understanding of the pathogenesis of parasite infection and host-parasite relationship at the molecular level. Profiling of biomarkers for parasitic diseases is a promising potential field, though further studies and optimization strategies are required, both in vitro and in vivo. Conclusion In this review, we discuss three approaches in the liquid biopsy including circulating cfDNA, miRNAs, and exosomes for diagnosis and evaluation of parasites and summarize circulating biomarkers in non-invasive samples during parasitic infections

Defining the Molecular Signal Pathways and Upstream Regulators in Cutaneous Leishmaniasis with Transcriptomic Data Approach

WOS:000613932200006PubMed: 33590982Leishmaniasis is a disease caused by the genus Leishmania spp., which are intracellular parasites. Depending on parasite species and host immune response, there are three basic clinical forms of the disease: cutaneous, mucocutaneous, and visceral leishmaniasis. Cutaneous leishmaniasis is a chronic disease and characterized by the presence of ulcerated skin lesions. The type of skin pathology seen during disease is determined in part by the infecting Leishmania spp., but also by a combination of inflammatory and antiinflammatory host immune response factors resulting in diverse clinical outcomes. in this study, it was aimed to determine the genes, molecular signaling mechanisms and biological functions of the molecules that play a role in the pathogenesis of the disease and immune response and determine host-parasite interactions in mice that are naturally resistant and susceptible to Leishmania major and Leishmania brazifiensis. For this, transcriptomic series GSE56029 was downloaded from "Gene Expression Omnibus" (GEO) data base, including expression profiling of twenty-four tissue samples that were recovered from both naive mice and mice (BALB/c, C57BL/6) infected with L.major and L.braziliensis. Then, "Differentially Expressed Genes" (DEGs) were identified by limma package in R script. FDR q 2 as threshold values were accepted in the analysis. Subsequently, functional and pathway enrichment analyses were performed for the DEGs by "Ingenuity Pathway Analysis" (IPA). For each of DEGs, p 1 were used and analyzed with the software program IPA 8.0. Ingenuity Pathway Analysis revealed the most enrichment pathways to be the inflammation, dendritic cell maturation and "Triggering Receptor Expressed on Myeloid Cells 1" (TREM-1) signal mechanisms and that the DEGs related to the regulation of immune system process were closely associated with the progress of cutaneous leishmaniasis. The upstream regulator analysis predicted that TNF-alpha, IFN gamma, IL-1 beta, IL-10RA and "Signal Transducer and Activator of Transcription-1" (STAT-1) are the regulators that explained gene expression changes causing biological activities in the tissues. Chemical compounds that may have anti-leishmanial effects were also identified in the study. in this study, the mechanisms belonging to the parasite species and host that determine the resistance/susceptibility phenotype were attempted to elucidate. Assessment of gene expression patterns, cytokine/chemokines, and signaling pathways in BALB/c and C57BL/6 mice infected with L.major and L.braziliensis will provide a better understanding of the potential mechanisms underlying infection from a genetic perspective. These results may guide for the future studies in terms of developing potential biomarkers for the diagnosis and prognosis prediction of cutaneous leishmaniasis and providing information about new treatment targets

Trends in the incidence and overall survival of multiple primary cancers in Turkey

WOS: 000549860600003PubMed: 32521930Purpose: Multiple primary malignant tumors (MPCs) are defined as at least two histologically distinct malignancies in one individual. We aimed to present the risk of developing multiple primary cancers and to give information about the periods and prediction data regarding survival outcome for MPCs. Methods: All patients with MPCs between 1992-2017 were included in this study. Patients were compared in terms of the primary cancer number, age at diagnosis of first primary cancer, gender, time interval after first cancer detected, cancer types seen mostly together, and the rate of MPCs during the years. Results: the total number of included patients was 117,139. the proportion of patients with MPCs during the follow-up period was 4.95% (n=5,796). Eighteen percent of the cases were synchronous and 82% metachronous. MPCs were most commonly detected in the gastrointestinal tract and were detected more commonly in men than in women (p<0.001). Patients receiving radiotherapy (RT) (p <0.001) and chemotherapy (CT) (p <0.001) had more MPCs than those who never received RT or CT. Survival in MPCs was worse than in single primary cancers. the 5- and 10-year survival rates were 28.1%, and 12.4%, respectively. Conclusion: We identified approximately 5% MPCs among 117,139 patients in our database. MPCs are more common at 60-69 years in males and at 50-59 years in females. in terms of the risk of MPCs, we should be aware of the cancers that have risk factors, habits, and genetic features commonly affecting primary cancers

Lipid profile in children with acute viral hepatitis A

Conclusions: It was shown that serum triglyceride and apo B level increased, but apo A-I level decreased in patients with AVH. While cholestasis lowers apo A-I level, severe hepatic damage lowers both apo A-I and LDL. These parameters return to normal levels within 30 days. An interesting relationship between ammonia and apo A-I deserves further investigations, speculatively focused on hepatocyte nuclear factor 4 alpha