Anthocyanin‑rich black carrot (Daucus carota ssp. sativus var. atrorubens Alef.) and red cabbage (Brassica oleracea) extracts incorporated biosensor for colorimetric detection of Helicobacter pylori with color image processing

In this work, we developed novel colorimetric biosensors consisting of anthocyanin-rich either black carrot (Daucus carotassp. sativus var. atrorubens Alef.) or red cabbage (Brassica oleracea) extracts for rapid, sensitive, and economic detectionof&nbsp;Helicobacter pylori&nbsp;(H. pylori). We comparatively prepared two test solutions as biosensors including anthocyanin-richblack carrot extract (Anth@BCE) and red cabbage extract (Anth@RCE), both of which fxed to pH 2.5 and investigated theircolorimetric responses based on electronic structure and electron density of anthocyanins. We successfully used anthocyaninrich BCE and RCE as natural pH indicators in detection of H. pylori and introduced their advantages like non-toxicity, easyaccessibility, and high stability compared to synthetic indicators. The BCE and RCE tests gave the best color change in thepresence of 103&nbsp;CFU/mL (at 60&nbsp;min) and 104&nbsp;CFU/mL (at 75&nbsp;min) H. pylori suspensions prepared in an artifcial gastric fuid.The limit of detection was down to 10&nbsp;CFU/mL for RCE and BCE tests by increasing incubation time (≥5&nbsp;h). We furthermade an additional study that color diferences in the colorimetric responses observed by naked eyes were supported bydigital image processing with RGB (Red Green Blue) and Delta-E (ΔE) analysis. It is confrmed that results evaluated bynaked eyes and digital image processing are well consistent with each other. These fndings proposed that these colorimetric tests can be implemented to pH dependent detection of various microorganisms and can be efectively transferred fromlaboratory work to clinics in the near future.&nbsp;</p

ORTOFİTALDEHİT İLE ORTOFİTALDEHİT-GÜMÜŞ NANOPARTİKÜL ve ORTOFİTALDEHİT GÜMÜŞ GRAFENOKSİT NANOPARTİKÜLLERİN ANTİMİKROBİYAL ETKİNLİĞİNİN KARŞILAŞTIRILMASI

Ülkemizde gastroenteroloji kliniklerinde sıklıkla invaziv prosedürler uygulanmakta ve maliyeti oldukça yüksek olmaktadır. Bu amaçla bu kliniklerde sterilizasyonda önemli yeri olan ortofitaldehit (OPA) ile nanopartiküller sentezlenmiş ve ortofitaldehit kullanım miktarı azaltılmak sureti ile elde edilen nanokompozitlerin, sterilizasyon etkinliğini arttırması hedeflenmiştir. OPA gümüş ve grafenoksit nanopartiküller sentezlenmiş ve etkin çapları (hidrodinamik çaplar) ile yüzey yükleri sırasıyla dinamik ışık saçılma (DLS) ve Zeta potansiyel (ZT) ölçümleri ile belirlenmiştir. Ortofitaldehit ve nanokompozitlerin Staphylococcusaureus (S. aureus) ATCC 25923, Escherichiacoli (E. coli) ATCC 35218 ve Candidaalbicans (C. albicans) ATCC 90028 standart suşlarına karşı antimikrobiyal etkinlikleri sıvı mikrodilüsyon yöntemi kullanılarakyüzde inhibisyon metodu ile hesaplanmıştır. OPA@AgNP’lerin STEM ile 10-25 nm boyutunda olduğu belirlenmiştir. OPA@AgNP ve OPA@Ag@GONK’lerin olarak sırasıyla ~430 ve ~415 nmabsorbans piki olduğu tespit edilmiştir. OPA@AgNP ve OPA@Ag@GONK’lerin yüzey yükü sırasıyla -33 mV ile -46 mV olarak belirlenmiştir. OPA@Ag@GONK’lerin hidrodinamik çapı ise 1250 nm civarında ölçülmüştür. Grafenoksit negatif yüklü bir malzemedir, üzerine negatif yüklü OPA@AgNP’ler(-33 mV) oluştuğunda yeni malzeme olan OPA@Ag@GONK’lerin yüzey yükü -46 mV’a çıkmıştır. Sonuçlar birbiri ile uyumludur.Test edilen tüm mikroorganizmalarda genel olarak sırasıyla OPA@Ag@GONK, OPA@AgNPve OPA arasında anlamlı bir fark (p&lt;0.001) olduğu görülmektedir. Sentezlenen nanokompozitler OPA’dan sentezlenen ilk nanokompozitler olma özelliğini taşımaktadır. Bununla birlikte tek başına OPA kullanımından daha yüksek antimikrobiyal etkinliğe sahip olmaları yönüyle de önem arz etmektedir.All invasive procedures in volve the contact of a medical device or surgical instrument witht hepatient's sterile tissue or mucosal surfaces. At this point, the greatest risk is that pathogenic microorganism senter the body and cause infection. In our country, such invasive procedure sarefre quently performed in gastroenterology clinics and their cost is quite high. For this purpose, nanoparticles were synthesized with orthophtaldehyde (OPA), which is important in sterilization in the seclinics, and it is aimed to increase the sterilization efficiency of the nanocomposite obtained by reducing the amount of orthophtaldehyde use. OPA silver and graphenoxide nanoparticles were synthesized and their effective diameters (hydro dynamic diameters) and surface charges were determined by dynamic light scattering (DLS) and Zetapotential (ZT) measurements, respectively. Antimicrobial activities of orthophtaldehyde and nanocomposites against Staphylococcusaureus (S. aureus) ATCC 25923, Escherichiacoli (E. coli) ATCC 35218 and Candidaalbicans (C. albicans) ATCC 90028 standard strains were calculated by percent inhibition method using liquid microdilution method.OPA@AgNPs were determined to be 10-25 nm in size by STEM. OPA@AgNPandOPA@Ag@GONKs was determined as ~430 and ~415 nm absorbance peaks, respectively. The surface charges of OPA@AgNPandOPA@Ag@GONKs were determined as -33 m Vand -46 mV, respectively. The hydrodynamic diameter of OPA@Ag@GONKs was measure daround 1250 nm. Graphenoxide is a negatively charged material. When negatively charged OPA@AgNPs (-33 mV) were formed on it, the surface charge of the new material OPA@Ag@GONKsincreased to -46 mV. The results are compatible withe ach other. Although C.albicansis at a higher rate, it is seen that there is a significant difference (p&lt;0.001) between OPA@Ag@GONK, OPA@AgNP and OPA in all tested micro organisms, respectively. Thesyn the size dnano composites are the first to be synthesized from OPA. However, it is also important in terms of having a higher antimicrobial activity than the use of OPA alone. In this context, nanocomposites synthesized using OPA can reduce the use of chemicals and allow a more effective dis infection with less active substance