Solar light efficient photocatalytic activity degradation of emergent contaminants by coated TiO2 nanoparticles

The photocatalytic degradation of different emergent contaminants in aqueous solutions has been studied by using oligomer-coated TiO2 nanoparticles under solar light irradiation. The oligomer was synthesized using methacrylic acid, ethylene glycol diglycidyl ether and imidazole. The material was characterized by SEM, DLS, FT-IR and FT-Raman and UV-vis spectroscopy. The photocatalytic behavior with the model azo-dye methyl orange was analyzed in different conditions (initial concentration and pH) and the kinetic parameters were described by using the Langmuir-Hinshelwood model. The reuse and photocorrosion of the nanoparticles was studied up to seven operative cycles. The oligomer-coated nanoparticles showed better performance than the uncoated ones due to an increment in absorption in the visible light region. The charge transfer process would form electron/hole pairs (e-/h+) with the holes localized on the organic ligands (most probably on imidazole residues from oligomers) and the electrons in the conduction band of TiO2. Supernatant safety after the photocatalytic treatment was examined by the lettuce seeds assay and no toxicity was found. The versatility of the material was studied by exposing it to different emergent contaminants (ciprofloxacin, trimethoprim and chloramphenicol).Fil: Villanueva, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Copello, Guillermo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Campodallorto, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentin

Smart release of antimicrobial ZnO nanoplates from a pH-responsive keratin hydrogel

A smart antibacterial biomaterial based on a keratin hydrogel with pH-dependent behavior and Zinc Oxide nanoplates as biocide agent has been developed. The pH of a chronic wound is basic due to bacterial metabolism. Originally shrank at acid pH, keratin hydrogels swell upon contact with a bacterial contaminated media leading to the release of the nanoparticles. The material has been thoroughly characterized by infrared spectroscopy, Raman, scanning electron microscope, swelling behavior, Differential scanning calorimetry, Small-angle X-ray scattering, rheology, antimicrobial activity and cytotoxicity. The results show that 5% of Zinc Oxide nanoparticles concentration is the optimum for wound dressing applications.Fil: Villanueva, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Cuestas, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Pérez, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Campodallorto, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Copello, Guillermo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentin

Calcium alginate beads reinforced with synthetic oligomers, linear polyethylenimine and Cu(II): structural stability and potential applications

Calcium alginate beads were reinforced with linear polyethylenimine (PEI), Cu(II) and synthetic oligomers derived from a diepoxide, methacrylic acid and imidazole, to increase the resistance to stirring and vibration for environmental applications. The FT-IR and Raman spectra of the beads confirmed the presence of the organic reactants and their interactions. The SEM images of the lyophilized beads with an excess of oligomers exhibited an ordered core structure surrounded by a shell. The elemental mapping by EDAX showed a homogeneous distribution of Ca(II) and Cu(II), and a density influenced by PEI. The beads more resistant to sonication were those with the highest oligomer content. The enzyme soybean peroxidase (SBP) was immobilized in the beads for an environmental application that requires a stable matrix from chemical and structural points of view. The synergistic action of the entrapped SBP and Cu(II) on H2O2 activation induced the removal of an azo dye from aqueous solutions. Free radicals and O2 were released from Cu(II)-sites in the presence of the peroxide. The content of the oligomers determined the efficiency of the beads on dye removal.Fil: Torres, Dimas Ignacio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Villanueva, María Emilia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Lazaro Martinez, Juan Manuel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Copello, Guillermo Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Campodallorto, Viviana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentin

Linear polyethylenimine-decorated gold nanoparticles: One-step electrodeposition and studies of interaction with viral and animal proteins

Polyethylenimine (PEI)-decorated gold nanoparticles (AuNP) were electrodeposited on conductive surfaces in one-step procedure. Solution-state NMR evidenced that chloride from PEI.HCl was partially exchanged by AuCl 4 − prior to AuNP formation. XPS studies indicated the presence of Au° together with the interaction between AuNP with nitrogen atoms of the PEI polymer. The particle size by DLS was 49 nm. The electrochemical behavior of bare electrode and of glassy carbon electrode modified with PEI-decorated AuNP was compared using [Fe(CN) 6 ] 3-/4- redox probe, to determine the potential role of the nanomaterial and of the polymer in the detection of proteins. The most relevant experimental variables from cyclic voltammetry (CV), square-wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS) were used for the characterization of protein uptake. The adsorption by electrostatic interaction between the biomolecules and the positively charged polymer (PEIH + ) affected negatively the current response (Ip) of the probe, especially when the negatively charged protein was involved. This platform resulted adequate to immobilize proteins and to characterize this process, for further applications as a tool in bioanalysis or biotechnology.Fil: Lazaro Martinez, Juan Manuel. Universidad de Málaga; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Byrne, Agustin Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Rodríguez Castellón, Enrique. Universidad de Málaga; EspañaFil: Manrique, Julieta Marina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales y Ciencias de la Salud - Sede Trelew. Laboratorio de Virología y Genética Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jones, Leandro Roberto. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales y Ciencias de la Salud - Sede Trelew. Laboratorio de Virología y Genética Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Campodallorto, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentin

Adsorption of Cd(II) and Pb(II) onto a one step-synthesized polyampholyte: Kinetics and equilibrium studies

A one step-synthesized polyampholyte, bearing carboxylate and 2-methylimidazole (2MI) groups, was tested as adsorbent for the removal of Pb(II) and Cd(II) from aqueous solutions. This material combines the benefits of synthetic polymers, such as high adsorption capacity and chemical stability, and the advantages of biosorbents in regard of costs and simplicity of the production.The short time needed to achieve the adsorption equilibrium indicated a chemical-reaction controlled process. A network expansion was predicted as a result of repulsive interaction between the fixed positive charges.Langmuir model presented the best fitting to isotherm equilibrium data, with a maximum adsorption capacity of 182mgg -1 for Cd(II) and 202mgg -1 for Pb(II). The metal removal was strongly dependent on pH, involving carboxylate and 2MI residues. An ion-exchange process for Pb(II) and Cd(II), combined with coordination for the later, were the most probable mechanism of interaction. The adsorption of 1.35ppm Cd(II) was 72±6% in well-water, and the adsorption of 0.50ppm Pb(II) was 62±5% in tap-water. The recovery figures for Cd(II) in 1% HNO 3 were optimal.Fil: Copello, Guillermo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Diaz, Luis Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Campodallorto, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentin

The Chemistry and Applications of Gem-Diols

Geminal diols or gem-diols are the product of the addition of water to a carbonyl group of either an aldehyde or a ketone. These compounds are rarely stable and seldom found in the liquid or solid state since the moisture balance is largely dependent on the structure. However, the family of organic chemical compounds with this gem-diol moiety has increased over the last years due to the multidisciplinary research studies dedicated to the chemical characterization of this functional group, including the assessement of those factors that confer stability to these molecules. Gem-diols participate in beneficial chemical reactions involved in the development of new therapeutic strategies. Gem-diols may also be of environmental concern; particularly, imidazole and pyridine molecules containing either carbonyl or its hydrated groups are widely used for the synthesis of metal complexes with relevance in Medicinal and Coordination Chemistry due to the ability of gem-diols to coordinate different transition metals ions (copper and cobalt) and heavy metals ions (lead, chrome and arsenic), thus being attractive molecules to be employed in the synthesis of new polymeric materials and/or metal complexes for the catalytic removal of pollutants and the design of single-molecule magnets. This chapter will describe the spectroscopic techniques available for the identification of this functional group in heterocyclic compounds containing carbonyl groups, such as pyridinecarboxaldehyde, vitamin-B6-related compounds and imidazolcaboxaldehydes, among others. The aim of this chapter is to show that the combination of both single-crystal X-ray diffraction and nuclear magnetic resonance allows determining the presence of the gem-diol group together with the factors governing the stability and the occurrence of this rare functionalization. This chapter will also focus on and discuss the applications of this chemical group in organic synthesis, atmospheric processes, and their effect on odorant receptors and on the catalytic sites of enzymes.Fil: Crespi, Ayelen Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Campodallorto, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Lazaro Martinez, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentin

One-pot preparation of SBP-PANI-PAA-ethylene glycol diglycidyl ether sensor for electrochemical detection of H 2 O 2

Soybean seed coat peroxidase (SBP) was used in combination with aniline (ANI), acrylic acid (AA), ethylene glycol diglycidyl ether (EGDE) and H2O2, to prepare a biosensor in one-pot synthesis strategy. SBP catalyzed the chemical polymerization of ANI initiated by H2O2, to give the conductive polymer (PANI) in acidic medium where acrylate acted as dopant. Further reactions between free radicals, AA and the diepoxy EGDE determined the entrapment of the SBP together with PANI in a hydrogel on the electrode surface, becoming wired for the amperometric detection of H2O2 at neutral pH and −100 mV vs Ag/AgCl. On the other hand, the absence of AA and EGDE in the reaction mixture led to an unstable sensor modifier and an erratic response to H2O2. The apparent Km for immobilized SBP in the flow system was 0.0763 mM, lower than the parameter for the free enzyme at pH 7.0. Quantitative analysis of H2O2 was carried out in a flow injection system (FIA); the calibration plot was linear in the range of 5.0–50 μM, with a limit of detection of 2.2 μM. A dental bleaching sample was successfully analyzed and the catalase test results demonstrated the specificity of the response.Fil: Torres, Dimas Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Miranda, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Campodallorto, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentin

Pectin-iron capsules: Novel system to stabilise and deliver lactic acid bacteria

The delivery of probiotics has been a challenge because microorganisms must overcome harmful environments both at a technological and at a physiological level. Encapsulation is one of the strategies to protect microorganisms against both problems. This work aimed at encapsulating probiotic Lactobacillus plantarum CIDCA 83114 in pectin-iron beads obtained by ionotropic gelation, enabling bacteria safe delivery and providing a source of iron and fibre. Microorganisms in the stationary phase were suspended in a 4% w/v pectin solution at pH 5, and the suspension, dripped into a 150 mM FeSO4 solution. The beads were freeze-dried and stored for 60 days at 4 °C. The morphology of the beads was observed by scanning electron microscopy. Bacterial culturability was determined after freeze-drying, and after the exposure to simulated saliva, gastric and intestinal conditions. The iron and pectin releases were also investigated in the same digestive conditions. Microorganisms were fully entrapped in smooth and spherical pectin-iron beads of ca. 1–2 mm diameter. Bacterial culturability did not decrease during storage. Encapsulation protected microorganisms against simulated digestive conditions, also enabling the complete release of iron and pectins in the gut. The results obtained support the safe delivery of both probiotic bacteria and iron to the gut. As iron deficiency still continuous to be a worldwide problem, using iron-pectin beads could be an adequate strategy to functionalise food products, contributing to attain the recommended iron intake.Fil: Ghibaudo, María Florencia. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Gerbino, Oscar Esteban. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Campodallorto, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; ArgentinaFil: Gomez Zavaglia, Andrea. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentin

Development and characterization of a new polyampholyte-surfactant complex applied to the solid phase extraction of bisphenol-A

This paper presents a material that has both hydrophilic and hydrophobic domains, obtained by combination of a polyampholyte with a surfactant. This material was fully characterized by different spectroscopic techniques and microscopy. Bisphenol-A (BPA) was chosen as a model molecule to study the interaction with compounds of intermediate polarity. We explored the kinetics and equilibrium of BPA on the surface of the polyampholyte?surfactant complex and found a significantly high loading capacity (2.02mmolg−1) and complete binding from solutions at concentration levels below 100 umol L−1. The complex was encapsulated in agarose gel to be used as solid phase for extraction of BPA from food simulants in contact with polycarbonate bottles under different treatments. Bisphenol was preconcentrated, extracted and analysed by liquid chromatography with an amperometric detector. The instrumental detection limit of the technique was 10 ugL−1, which was lowered to 0.14 ugL−1 by the preconcentration step. The BPA released from baby bottles was 2.1 ngcm−2 (tn−1: 0.1) in the first use with distilled water.Fil: Lazaro Martinez, Juan Manuel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leal Denis, Maria Florencia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Denaday, Lisandro Roberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Campodallorto, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentin

Single step synthesis of a polyhydroxy ether and its optimization to adsorption of a textile dye

An ion exchange resin was designed by combining different ratios of ethylene glycol diglycidyl ether (EGDE) and imidazole (IM). The aim of the research was to obtain in a single step a resin with permanent positive charges. The strategy was to use the IM in a dual way, first as a curing agent during the synthesis and as carrier of the permanentpositive charge in the final product. This novel procedure avoid further modification of the resin, reducing time and cost of production. The material with 1:0.3 EGDE:IM molar ratio achieved high adsorption capacity for the textile dye, Remazol Black B (qmax: 1.33 × 103 mg g-1) and good performance in a continuous adsorption system. The water uptake was 592% and pH independent. 1H-NMR confirmed the covalent binding of IM to the polyelectrolyte chain, and the FTIR results evidenced epoxy opening. The shielding of the positive charge resulting from the electrostatic interaction of this polyelectrolyte with Remazol Black B induced some degree of chain shrinkage, according to SEM images and to the visual inspection. In the attempt to optimize the performance of the fixed bed adsorption system an experimental design was carried out. The multilinear regression model allowed the acquisition of a deeper knowledge of the experimental space and opening the way to further improvement of the process.Fil: Torres, Dimas Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Lazaro Martinez, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica. Química Orgánica II; ArgentinaFil: Copello, Guillermo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Campodallorto, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentin