An integrated analysis of micro- and macro-habitat features as a tool to detect weather-driven constraints: a case study with cavity nesters

The effects of climate change on animal populations may be shaped by habitat characteristics at both micro- and macro-habitat level, however, empirical studies integrating these two scales of observation are lacking. As analyses of the effects of climate change commonly rely on data from a much larger scale than the microhabitat level organisms are affected at, this mismatch risks hampering progress in developing understanding of the details of the ecological and evolutionary responses of organisms and, ultimately, effective actions to preserve their populations. Cavity nesters, often with a conservation status of concern, are an ideal model because the cavity is a microenvironment potentially different from the macroenvironment but nonetheless inevitably interacting with it. The lesser kestrel (Falco naumanni) is a cavity nester which was until recently classified by as Vulnerable species. Since 2004, for nine years, we collected detailed biotic and abiotic data at both micro- and macro-scales of observation in a kestrel population breeding in the Gela Plain (Italy), a Mediterranean area where high temperatures may reach lethal values for the nest content. We show that macroclimatic features needed to be integrated with both abiotic and biotic factors recorded at a microscale before reliably predicting nest temperatures. Among the nest types used by lesser kestrels, we detected a preferential occupation of the cooler nest types, roof tiles, by early breeders whereas, paradoxically, late breeders nesting with hotter temperatures occupied the overheated nest holes. Not consistent with such a suggested nest selection, the coolest nest type did not host a higher reproductive success than the overheated nests. We discussed our findings in the light of cavity temperatures and nest types deployed within conservation actions assessed by integrating selected factors at different observation scales

Evolutionary significance of antiparasite, antipredator and learning phenotypes of avian nest defence

Avian nest defence, which is expected to serve both antiparasite and antipredator functions, may benefit or be detrimental to birds, although selective forces that potentially operate on nest defence have not been quantified as a whole. Together with fitness values, we analysed two traits of nest defence, intensity and plasticity, in two distantly related passerine species, yellow warbler (Setophaga petechia) in North America and reed warbler (Acrocephalus scirpaceus) in Europe, both favourite host species for brood parasites. Breeders that escaped parasitism were the most vocal among reed warblers, whereas there was no specific defence phenotype that predicted prevention of parasitism in yellow warblers. Breeders that escaped nest predation were, in both species, those with the most distractive response at the first exposure to a nest-threatening event, such as the experimental predation or parasitism simulated at the nest. However, increasing defence intensity benefited yellow warblers but was detrimental to reed warblers, because intense defence responses attracted predators. Adaptiveness of nest defence was revealed by nest defence phenotypes when examined in concert with the seasonal fitness (i.e. measures of reproductive success). Results revealed selective forces favoured yellow warblers with strong defence phenotypes. Opposite forces were instead revealed among reed warblers whose favoured phenotypes were strong, yet less flexible, defenders

Effects of nest and colony features on lesser kestrel (Falco naumanni) reproductive success

Lesser kestrel is a facultative colonial raptor mostly breeding in man-made structures. During 2009-2011 we checked the fate of 545 nests found in 18 colonies located in south-eastern Sicily. We determined the reproductive success of breeding pairs by analysing the survival time of each egg to hatching (n = 2495) and each nestling to fledging (n = 1849) with the Linear Hazard model of survival times. We determined whether egg and nestling survival differed between years with a Gehan-Wilcoxon test. By Cox regressions, we related the survival times with nest and colony features. Egg and nestling survival times showed a strong annual effect. The two reproductive stages of lesser kestrel when controlled for the annual effect demonstrated a significant effect for some nest and colony features. Most of them, like height from the ground, nest depth, reused nests and the presence of jackdaws, are likely related to the predation pressure faced by lesser kestrel nests. Westward and southward nests had a better egg survival respect to those in the cold sides and in the interior of buildings. The location of colony in the agricultural plain and human disturbance ranked as the most important variables, with core location of a colony and high disturbance levels negatively affecting the reproductive success of lesser kestrel in the Gela Plain, so urging a more environment-aware land use management of this Important Bird Area

Reed Warbler Hosts Do Not Fine-Tune Mobbing Defenses During the Breeding Season, Even When Cuckoos Are Rare

Hosts of brood parasitic cuckoos often employ mobbing attacks to defend their nests and, when mobbing is costly, hosts are predicted to adjust their mobbing to match parasitism risk. While evidence exists for fine-tuned plasticity, it remains unclear why mobbing does not track larger seasonal changes in parasitism risk. Here we test a possible explanation from parental investment theory: parents should defend their current brood more intensively as the opportunity to replace it declines (re-nesting potential), and therefore “counteract” any apparent seasonal decline to match parasitism risk. We take advantage of mobbing experiments conducted at two sites where reed warblers (Acrocephalus scirpaceus) experience (in Italy), or do not experience (in Finland), brood parasitism. We predicted that mobbing of cuckoos should be higher overall in Italy, but remain constant over the season as in other parasitised sites, whereas in Finland where cuckoos do not pose a local threat, we predicted that mobbing should be low at the beginning of the season but increase as re-nesting potential declined. However, while cuckoos were more likely to be mobbed in Italy, we found little evidence that mobbing changed over the season at either the parasitized or non-parasitized sites. This suggests that re-nesting potential has either little influence on mobbing behavior, or that its effects are obscured by other seasonal differences in ecology or experience of hosts

Ritual displays by a parasitic cuckoo: nuptial gifts or territorial warnings?

In the sexual selection framework, nuptial gifts are materials a donor provides to a receiver that can increase the donor's fitness. In specific cases, sharing crucial information may be a nonmaterial nuptial gift. To investigate this hypothesis, we focused on the common cuckoo, Cuculus canorus, an obligate avian brood parasite whose reproduction costs of females are mainly related to finding host nests needed to lay their eggs. Nest searching is assumed to be conducted only by females. We hypothesized that males could contribute by transferring information on nest locations to females as a nonmaterial nuptial gift. Here, we show the results of a first step in this direction, in which we identified any behaviour potentially conveying information on nest abundance in the surrounding area, that is, behaviours whose frequency varied with host nest density. We conducted our investigation in a marshland area within the Po Plain (Italy), where we recorded both visual displays of cuckoos at perching sites, by using camera traps, and nest abundance of two of the most parasitized cuckoo host species, the reed warbler, Acrocephalus scirpaceus, and great reed warbler, Acrocephalus arundinaceus, by systematic nest monitoring. We found that male cuckoos adopted a certain posture, wing drooping, and tended to keep their tails up more frequently in areas with the highest versus lowest host nest densities. This is consistent with these behaviours acting as potential signals codifying information on nest abundance in the area. We finally discuss the implications of our findings for the mating choices of female cuckoos and the study directions warranted to reveal whether these displays and information transfer may be included as new elements of the sexual selection framework. (c) 2023 The Author(s). Published by Elsevier Ltd on behalf of The Association for the Study of Animal Behaviour. This is an open access article under the CC BY license (http://creativecommons.org/licenses/ by/4.0/)

Decoupled Acoustic and Visual Components in the Multimodal Signals of the Common Cuckoo (Cuculus canorus)

Because of its parasitic habits, reproduction costs of the common cuckoo (Cuculus canorus) are mostly spent in pre-laying activities. Female costs are limited to searching host nests and laying eggs, whereas, males spend time in performing intense vocal displays, possibly with territorial purpose. This last aspect, together with a sexual plumage dimorphism, points to both intra- and inter-sexual selections operating within this species. One element triggering sexual selection is a differential fitness accrued by different phenotypes. Before analyzing possible sexual selection mechanisms operating in cuckoos, it is therefore necessary to verify whether there is a variability among male secondary characters by describing and quantifying them. Here we aimed to quantitatively characterize the main two potential candidates of sexual selection traits, i.e., calls and displays, shown by males at perches. During the 2019 breeding season, in a site within the Po Plain, we both audio and video recorded cuckoo males at five different perches. We analyzed acoustic variables as well as display sequences searching for potential correlations. We found a significant variation among calls that could be clustered into four vocal types. We also found that no visual displays were associated with vocal displays; cuckoo males were either vocal and motionless or soundless and active. We discuss our results under the perspective of the potential value of sexual selection in brood parasites and its role in its parasitic habit

Decoupled Acoustic and Visual Components in the Multimodal Signals of the Common Cuckoo (Cuculus canorus)

Because of its parasitic habits, reproduction costs of the common cuckoo (Cuculus canorus) are mostly spent in pre-laying activities. Female costs are limited to searching host nests and laying eggs, whereas, males spend time in performing intense vocal displays, possibly with territorial purpose. This last aspect, together with a sexual plumage dimorphism, points to both intra- and inter-sexual selections operating within this species. One element triggering sexual selection is a differential fitness accrued by different phenotypes. Before analyzing possible sexual selection mechanisms operating in cuckoos, it is therefore necessary to verify whether there is a variability among male secondary characters by describing and quantifying them. Here we aimed to quantitatively characterize the main two potential candidates of sexual selection traits, i.e., calls and displays, shown by males at perches. During the 2019 breeding season, in a site within the Po Plain, we both audio and video recorded cuckoo males at five different perches. We analyzed acoustic variables as well as display sequences searching for potential correlations. We found a significant variation among calls that could be clustered into four vocal types. We also found that no visual displays were associated with vocal displays; cuckoo males were either vocal and motionless or soundless and active. We discuss our results under the perspective of the potential value of sexual selection in brood parasites and its role in its parasitic habit