The Effects of Weaning Strategy on the Physiology and Performance of Beef Calves

Two experiments examined growth performance and physiological measures of stress in pre- and postweaned Angus steers (313 ± 24.5 kg; n = 48/Exp.), where steers were fitted with (YD) or without (ND) an anti-suckling device (Exp. 1), or provided (YS) or not provided (NS) a grain supplement (Exp. 2) for 7 d and weaned by fenceline (FS) or total separation (TS). Steers in Exp. 1 were weighed and bled on d 0, 3, 7 10, 14, 21, and 42, and in Exp. 2, on d 0, 7, 10, 14, and 21 and provided a grain supplement on d 7-21. In Exp. 1, weight gain was not different (P = 0.74) between ND and YD steers during preweaning. The YD-FS steers lost weight (P = 0.01) by d 10 compared with YD-TS steers. Hematocrit (Hct) increased (P = 0.04) in YD but not ND steers on d 3. Neutrophil:lymphocyte ratio (N:L) increased (P \u3c 0.01) in all steers by d 7. Cortisol values in YD-FS steers were higher (P \u3c 0.05) compared with YD-TS steers on d 10 and 21. The ND-FS steers had higher (P = 0.04) interferon-gamma (IFN) concentrations on d 10 compared with all YD steers. Haptoglobin (HAP) values increased (P \u3c 0.01) in all steers by d 3. The FS steers had higher (P \u3c 0.01) ceruloplasmin (CER) values by d 10 than TS steers regardless of preweaning treatment. Ovalbumin-specific IgG increased (P \u3c 0.01) in all steers 10 d following its administration. In Exp. 2, NS-TS steers lost weight (P \u3c 0.01) between d 7 and 10 compared with the remaining steers. The YS steers had higher cortisol, N:L and CER on d 7 compared with NS steers. Moreover, NS steers had higher (P \u3c 0.01) Hct on d 10 than YS steers. Based upon physiological and growth performance data, it may be concluded that use of an anti-suckling device prior to weaning does not improve the animals’ well-being and, providing a grain supplement beginning 7 d prior to weaning may temper the animals’ stress response due to weaning when total separation is employed

Early elimination of cyclosporine in kidney transplant recipients receiving sirolimus prevents progression of chronic pathologic allograft lesions

Cyclosporine elimination in a regimen including sirolimus has been shown to be a safe and effective approach to improve graft function. Nevertheless, it is still unknown whether the functional benefit of CyA withdrawal coincides with a subsequent reduction in histologic lesions of chronic damage or development of chronic allograft nephropathy. This consideration would forecast a reduction in the rate of long-term graft loss. We analyzed 114 graft biopsies from a subgroup of 57 patients that had been included in a randomized study to eliminate CyA at 3 months posttransplant from a regimen including sirolimus either in group A CyA + SRL vs group B of SRL with CyA elimination at 3 months. Every patient had two biopsies, one at transplantation and another at 1 year. The biopsy reading was performed in a blinded manner by a central pathologist using the Banff 1997 and the CADI classifications. A significantly lower rate of progression of tubular and interstitial chronic lesions between basal and 1-year biopsies was observed for group B patients. In addition, the incidence of new cases of chronic allograft nephropathy during the first year was significantly lower in the group in which CyA had been eliminated at 3 months posttransplant. We conclude that early elimination of CyA in the first months posttransplant, when SRL is used as the main immunosuppressant, reduces the appearance or worsening of chronic histologic lesions, probably as a consequence of long-term CyA toxicity prevention