PET uptake in H727 xenografts.

<p>Uptake of FDG (upper panel) and FLT (lower panel) after treatment of H727 xenografts with everolimus or vehicle. N = 8–10 tumors/group. *) P<0.05 vs. control group on same day, #) P<0.05 and ##) P<0.01 vs. baseline of same group. P-values are Bonferroni corrected.</p

Study design.

<p>Study design.</p

Early PET correlated to later tumorgrowth.

<p>Correlations of individual tumor uptake (SUVmean) day 1 and day 3 and subsequent tumor growth until day 7 and 10. Left panel: mean uptake of FDG at day 3 correlated significantly with tumor growth until day 10, (n = 10). Right panel: mean uptake of FLT at day 1 correlated significantly with tumor growth until day 7 and FLT uptake at day 3 correlated with tumor growth until both day 7 and 10 (n = 8 tumors). Tumor growth is expressed as volume relative to baseline, 95% confidence intervals are shown. Correlations with SUVmax show same trend (not shown).</p

MTT Assay.

<p><i>In vitro</i> inhibition of neuroendocrine tumor cell proliferation by everolimus using MTT assay.</p

Correlation between SUVmax and tumor growth.

<p>Tumor growth assessed by ratio Day 7/baseline compared to uptake of [18F]FLT (upper panels) and [18F]FDG (lower panels) at 24 h (right panels), 48 h (middle panels) and 7 days (left panels). N = 6 tumors in [18F]FLT control group, N = 10 tumors in [18F]FLT treatment group, N = 12 tumors in [18F]FDG control group and N = 12 tumors in [18F]FDG treatment group.</p

Chemical structure of the nicotinamide phosphoribosyltransferase inhibitor APO866.

<p>Chemical structure of the nicotinamide phosphoribosyltransferase inhibitor APO866.</p

Correlation between Ki67 expression and [18F]FLT uptake. A

<p>Correlation of Ki67 protein expression measured by immunohistochemistry with [18F]FLT uptake measured as SUVmean (upper panel). Lower panel compares Ki67 protein expression in the APO866 (N = 10) and control group (N = 6) at the end of the 7 day treatment period. Values are stated as mean±SEM. <b>B</b> Representative images of Ki67 stains of an APO866 treated (left) and a control (right) mouse in 40×magnification.</p

Tumor growth of APO866 and vehicle treated A2780 xenografts.

<p>The effects of APO866 on the growth of A2780 tumor xenografts were measured. Tumor volume was determined by microCT. Mice were treated with APO866 (15 mg/kg) or vehicle ip twice daily for 7 days. N = 22 tumors in treatment group and N = 18 in control group. Values are stated as mean±SEM.</p