1,732 research outputs found
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Virulence factors in vancomycin-resistant and vancomycin- susceptible Enterococcus faecalis from Brazil
Enterococci are members of commensal flora of animals and insects, but are also important opportunistic pathogens. Our objective was to observe if there was any difference of virulence in several groups of E. faecalis, mainly between vancomycin-resistant E. faecalis (VREFS) of colonization and infection. VREFS and vancomycin-sensitive E. faecalis from Brazil were screened for the presence of virulence factor genes. Phenotypic assays were used to assess in vitro expression, to understand the pathogenic potential of these isolates and to determine whether a correlation exists between virulence and antibiotic resistance. Different virulence profiles were found suggesting that the disseminating clone may have generated several variations. However, our study showed that one constellation of traits appeared most commonly: gelatinase, aggregation substance and esp (GEA). These factors are important because they have been implicated in cell aggregation and biofilm formation. Biofilm formation may promote the conjugation of plasmids harboring resistance and virulence genes, enhancing the probability of entry of new resistance genes into species. Curiously, the profile GEA was not exclusive to VREFS, it was the second most observed in VSEFS isolates from colonization and infection in hospitalized patients and also from rectal swabs of healthy volunteers. Such strains appear to represent the entry gateway to new resistance genes into E. faecalis and may contribute to the spreading of E. faecalis mainly in hospitals
Habitat characteristics or protected area size: What is more important for the composition and diversity of mammals in nonprotected areas?
The margins of protected areas are usually considered to have greater forest degradation, and given that most mammals live outside protected areas, researchers and conservation practitioners are increasingly recognizing that nonprotected areas must be incorporated into conservation strategy. However, the strategy used to manage these areas still involves increasing the size of protected areas, while not considering the habitat characteristics and requirements of the species. In this study, during a 3-year period, camera trap and habitat characteristic surveys were used to estimate composition, diversity, and habitat characteristics of mammals to determine habitat characteristics or increase the size of protected areas what should be considered first for mammals’ conservation in a nonprotected area near the Huangshan Mountains in Anhui Province, China. From June 2017 to October 2019, 18 species of mammals were recorded, more than in any other protected area nearby. The linear model analysis results showed that habitat characteristics of mammals were different and showed a significant correlation with their relative abundance. Most species were related to vegetation characteristics, except primates (Macaca thibetana), and rodents (Leopoldamys edwardsi). Therefore, to establish conservation policies for nonprotected areas, habitat characteristics should be of prime concern, followed by increasing the size of protected areas to provide effective refuge areas for species conservation
Editorial: Antimicrobial Resistance as a Global Public Health Problem: How Can We Address It?
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Germination changes the isoflavone profile and increases the antioxidant potential of soybean.
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Sequence Analysis of Enterococcus faecium Strain 10/96A (VanD4), the Original Vancomycin-Resistant E. faecium Strain in Brazil
Enterococcus faecium strain 10/96A (VanD4) was the first vancomycin-resistant enterococcus (VRE) isolated in Brazil. Subsequent Brazilian VRE strains have all had the VanA phenotype. Multilocus sequence typing showed that strain 10/96A was isolated sporadically, has a unique sequence type (ST 281), and was not the progenitor of the VRE strains isolated from hospital outbreaks in Brazil
Avaliação de compostos orgânicos em plantas de videiras jovens.
Este trabalho tem como objetivo avaliar o efeito de diferentes formas de misturas de compostos orgânicos sobre a cultura da videira
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