Eco-friendly synthesis, characterization, in vitro and in vivo anti-inflammatory activity of silver nanoparticle-mediated Selaginella myosurus aqueous extract

Introduction: There is emerging interest in medicinal plants in the biomedical field, due to their multitude of chemicals which show anti-inflammatory, antimicrobial, antiviral, or antitumoral potential. Research on medicinal plants has shown that nanotechnology could offer new solutions in the quality control, delivery aspects, or in sustaining herbal biological activities. This work reports on the preparation and characterization of silver nanoparticle-mediated Selaginella myosurus plant extract. Methods: Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, powder X-ray diffraction, energy dispersive X-ray spectroscopy, high-resolution scanning electron microscopy, high-resolution transmission electron microscopy, and selected area electron diffraction have been used to characterize the prepared silver nanoparticles. The synthetic stability was studied by varying concentrations and pH of reactants. Egg albumin denaturation and carrageenan-induced rat paw edema model were used to ascertain the anti-inflammation. Results: Ultraviolet-visible spectroscopy gave plasmon resonance ranging between 420 and 480 nm while Fourier transform infrared spectroscopy proved nano interface functionalized with organics. The powder X-ray diffraction pattern is in agreement with silver and silver chloride nanoparticles of crystallite size 33.7 nm and 44.2 nm for silver and silver chloride, respectively. Energy dispersive X-ray spectroscopy enables elemental characterization of the particles consisting of silver and silver chloride among main elements. Spherical silver grain of 58.81 nm average size has been depicted with high-resolution scanning electron microscopy and high-resolution transmission electron microscopy. Inhibitions of 99% and 60% were obtained in vitro and in vivo, respectively. Conclusion: The albumin denaturation and carrageenan-induced rat hind paw edema model to assess the anti-inflammatory potential of generated nanoparticles suggests that the silver nanoparticles may act as reducing/inhibiting agents on the release of acute inflammatory mediators. Hence, this work clearly demonstrated that silver nanoparticles mediated-Selaginella myosurus could be considered as a potential source for anti-inflammatory drugs

Preserving culinary heritage and promoting sustainability: an overview of botanical nutrition regarding herbs and spices used on the territory of today’s Cameroon

This is an author's accepted manuscript of an article published by Springer in SN Social Sciences, available online: https://doi.org/10.1007/s43545-024-00910-z The accepted manuscript may differ from the final published version. For re-use please see Springer's terms and conditions.Herbs and spices are not merely condiments; they are the essence of our cultural heritage, infusing our cuisine with flavors that do more than tantalize the palate, they offer a spectrum of health benefits. This study meticulously reviews 59 botanicals, 44 spices and 15 herbs, cherished in Cameroon, presenting a treasure trove of ethnobotanical and ethnonutritional wisdom. Fruits emerge as the most favored part of plants used in spices. Herbs often hail from Asia and Europe, and spices from the lush tropics and subtropics of Africa. This review casts a spotlight on the critical role these plants play in culinary artistry and health promotion, thanks to their rich array of bioactive compounds. It navigates through the complexities of sustainable management and conservation, advocating for the longevity of these botanicals for the enrichment of future generations. The paper calls for rigorous scientific research to substantiate the health claims associated with these natural wonders and promotes sustainable harvesting and market expansion. It underscores the necessity of educating the public about the value of these plants. In conclusion, the paper urges continued research into the scientific validation of health benefits, the adoption of sustainable practices, the exploration of value-added products, market development strategies, and heightened public awareness. This research aims to lay a robust foundation for future endeavors, aspiring to holistically manage health and well-being through the sustainable harnessing of these invaluable natural resources.Published onlin