4 research outputs found
Differential Behavior Between Isolated and Aggregated Rabbit Auricular Chondrocytes on Plastic Surfaces
A knowledge of the behavior of chondrocytes in culture is relevant for tissue engineering. Chondrocytes dedifferentiate to a fibroblast-like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured in suspension. In this report a description is given of how when chondrocyte aggregates formed in suspension are next seeded on plastic, most of them attach as round or polygonal cells. This morphological differentiation, with synthesis of type II collagen, is stable for long culture periods. This simple method can be of use as a model for studies of chondrocyte behavior on plastic. The results indicate that in addition to culture conditions, such as cell isolation method or cell density, chondrocyte behavior on plastic depends on the presence of aggregates
Effects of Extracellular Matrix on the Morphology and Behaviour of Rabbit Auricular Chondrocytes in Culture
Isolated chondrocytes dedifferentiate to a fibroblast-like shape on plastic substrata and proliferate extensively, but rarely form nodules. However, when dissociation is not complete and some cartilage remnants are included in the culture, proliferation decreases and cells grow in a reticular pattern with numerous nodules, which occasionally form small cartilage-like fragments. In an attempt to reproduce this stable chondrogenic state, we added a cartilage protein extract, a sugar extract, and hyaluronan to the medium of previously dedifferentiated chondrocytes. When protein extract was added, many cartilaginous nodules appeared. Hyaluronan produced changes in cell phenotype and behaviour, but not nodule formation. Protein extract has positive effects on the differentiation of previously proliferated chondrocytes and permits nodule formation and the extensive production of type-II collagen. A comparison with incompletely dissociated chondrocyte cultures suggests that the presence of some living cells anchored to their natural extracellular matrix provides some important additional factors for the phenotypical stability of chondrocytes on plastic surfaces. In order to elucidate if it is possible that the incidence of apoptosis is related to the results, we also characterized the molecular traits of apoptosis
Culture of Differentiated Adult Rabbit Auricular Chondrocytes
P. 54Chondrocytes dedifferentiate to a fibroblast-like phenotype
on plastic surfaces. Dedifferentiation is reversible if these
cells are then cultured embedded in gels as alginate, agarose
or collagen. Chondrocytes cultured in suspension on a nonadherent
surface are also known to form aggregates of
differentiated cells. The knowledge of chondrocyte behavior
in culture is relevant for tissue engineering purposes. In
this report we describe a simple method to culture differentiated
or redifferentiated rabbit auricular chondrocytes
on plastic surfaces with a stable phenotype. When chondrocyte
aggregates formed in suspension are next seeded on
plastic surfaces, most of them attach to the plastic as round
or polygonal cells, and this morphological differentiation,
confirmed by the presence of type II collagen, is stable for
long culture periods. We also report that the addition of
aggregates to monolayer cultures of dedifferentiated
chondrocytes results in their redifferentiation, as is shown
by their morphological changes and the synthesis of type II
collagen. Therefore, this simple method can be useful for
the study of chondrocyte behavior on plastic surfaces and
for redifferentiating previously proliferated chondrocytes
in tissue engineering techniques. Furthermore, these results
demonstrate that, in addition to culture conditions such as
cell isolation method or cell-density, chondrocyte behavior
on plastic depends on the presence or absence of aggregates
resulting from the dissociation processS
Differential Behavior Between Isolated and Aggregated Rabbit Auricular Chondrocytes on Plastic Surfaces
P. 86-92A knowledge of the behavior of chondrocytes in culture is relevant for tissue engineering. Chondrocytes dedifferentiate 10 a fibroblastĀ· like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured in suspension. In this report a descripĀ·
tion is given of how when chondrocyte aggregates formed in suspension are next seeded on plastic, most of them attach as round
or polygonal cells. This morphological differentiation, with synthesis of type II collagen, is stable for long culture periods. This simĀ· pie method can be of use as a model for studies of chondrocyte behavior on plastic. The results indicate that in addition to culture conditions, such as cell isolation method or cell density, chondrocyte behavior on plastic depends on the presence of aggregatesS