Acute prostatitis as an uncommon presentation of brucellosis

The present report concerns a 46-year-old man who presented with acute prostatitis due to Brucella melitensis infection. He was first treated with doxycycline and ciprofloxacin, but after 3 months he was admitted again with the same diagnosis. The relapse was probably related to ciprofloxacin use, or the length of treatment not being sufficient. The patient was successfully treated with a combination of doxycycline and rifampin for 3 months. In conclusion, prostatitis due to Brucella, such as spondylitis, meningoencephalitis and endocarditis, should be treated for longer courses

Nosocomial respiratory syncytial virus infections in three cases in a bone marrow transplantation unit Kemik iliǧi nakli ünitesinde yatan üç olguda nozokomiyal solunum sinsityal virus enfeksiyonu

Here we report the spread of respiratory syncytial virus (RSV) infection among three patients, who were hospitalized in an adult hematopoetic stem cell transplantation (HSCT) unit because of hematologic diseases, and effects of RSV infection on post-transplant outcome. The patients were placed into reverse isolation for administration of preparative regimens (high dose chemotherapy) in HSCT unit with high-energy particulate air (HEPA)-filtered single rooms. First case was a 62 years-old man with advanced multiple myeloma, which was refractory to multiple line treatment with high dose steroid including regimens and with secondary acute myelogenous leukaemia (AML); second case was a 45 years-old male patient with multiple myeloma, who had undergone autologous HSCT following high dose chemotherapy; third case was a 52 years-old man with AML that was refractory to multiple line treatment and had undergone allogeneic HSCT from a HLA-matched unrelated donor. Nasopharyngeal aspirate samples were collected from the patients in order to search for RSV positivity. RSV was investigated by in-house nested polymerase chain reaction (PCR) in the first patient and by direct antigen detection method (Monofluoscreen RSV-Biorad, France) in the others. First case had clinical picture of RSV infection just on the HSCT day when high dose chemotherapy has already been given. As RSV-RNA analysis yielded positive result, peroral ribavirin was initiated. Engraftment did not occur in this patient. He developed severe respiratory failure which necessitated mechanical ventilatory support, however, he has succumbed. After the detection of RSV positive index case, weekly screening of RSV in other five patients in the same unit had been performed. Following the first case, after nine and 17 days, respectively, RSV positivity was detected in two more patients. While clinical signs and symptoms of RSV infection developed in second case, third case remained asymptomatic. Both of the following patients had received ribavirin very early at first RSV positivity and recovered from RSV infection. Engraftment did not occur in the last patient who had undergone allogeneic HSCT from a HLA-matched unrelated donor and a second HSCT was performed. As a result, in HSCT patients, early diagnosis of RSV infection by PCR analysis may provide support to postpone immunosupressive treatment and help assesment of the management

Is isepamicin a solution for aminoglycoside resistance?

Aminoglycosides are commonly used antibiotics especially in hospital-acquired infections. As a result of their widespread usage, resistance to these antibiotics is increasing. Isepamicin is a newly developed aminoglycoside and it is claimed that resistance to this antibiotic will develop in lower frequency in comparison with other aminoglycosides. This study was conducted to evaluate the resistance patterns of Gram-negative isolates to aminolycosides and to compare isepamicin with other aminoglycosides. All Gram-negative bacteriae isolated from blood and other cultures during the years 2000-2001 were tested against gentamicin, tobramycin, netilmicin, amikacin and isepamicin. The in-vitro susceptibility tests were performed by disk diffusion method. In total 3683 Gram-negative isolates were studied. According to our results, netilmicin (66.4%) was the most effective aminoglycoside. It was followed by isepamicin (64%), amikacin (63%), tobramicin (55.9%) and gentamicin (51.9%). Netilmicin was also the most effective aminoglycoside against Gram-negative bacteriae isolated from blood culture. Amikacin (62.6%) and isepamicin (62.5%) were equally effective against these isolates. In conclusion, netilmicin was the most effective aminoglycoside against Gram negative bacteriae. The resistance to isepamicin was significant

Pathogens isolated from deep soft tissue and bone in patients with diabetic foot infections

Background: We sought to determine the similarity of pathogens isolated from soft tissue and bone in patients with diabetic foot infections. It is widely believed that soft-tissue cultures are adequate in the determination of causative bacteria in patients with diabetic foot osteomyelitis. The culture results of specimens taken concurrently from soft-tissue and bone infections show that the former does not predict the latter with sufficient reliability. We sought to determine the similarity of pathogens isolated from soft tissue and bone in patients with diabetic foot infections

Genetic subtypes of human immunodeficiency virus type 1 (HIV-1) in Istanbul, Turkey

Background: Epidemiological surveillance of HIV-1 subtypes is an important and ongoing element of preparation for global antiviral interventions