Data_Sheet_1_In vivo evidence of the prevents DSS-induced colitis of Lactiplantibacillus plantarum L15.ZIP

Ulcerative colitis (UC) is challenging to treat and severely impacts patients and families. A previous study reported immunomodulatory and reduction of pro-inflammatory properties for the Lactiplantibacillus plantarum L15. This study aimed to analyze the preventive properties and mechanistic actions in an in vivo colitis model. The histopathological alteration, inflammation cytokines, and intestinal barrier function were analyzed. Subsequently, the cecal gut microbiota contents and products from different groups were detected. Finally, gene expressions related to the NF-κB signaling process were evaluated. L. plantarum L15 significantly decreased disease activity index (DAI), myeloperoxidase activity (MPO), pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) level, and increased weight change, colon length, and production of inflammation-suppressing cytokines. Furthermore, this strain supplementation substantially increased ZO-1, Occludin, and Claudin-1, and MUC2 mRNA expression levels with a corresponding decrease in serum lipopolysaccharide and D-lactic acid contents. In addition, L. plantarum L15 improved gut microbiota composition and increased short-chain fatty acid (SCFAs) in the colon content, which significantly reduced the transfer of NF-κB p65 to the nucleus. Our findings provide a theoretical basis for L. plantarum L15 as a preventive candidate for UC.</p

Additional file 1: Figures S1–S6. of Facile Fabrication of Bi2WO6/Ag2S Heterostructure with Enhanced Visible-Light-Driven Photocatalytic Performances

The EDS, BET surface area, and Zeta potential analysis for the as-formed heterostructures, the XRD pattern of Ag2S, and the temporal evolution of Rh B absorption spectra over Bi2WO6/Ag2S heterostructure at different pH values. Figure S1. Elemental mapping and EDX spectra of the Bi2WO6/Ag2S heterostructure. Figure S2. EDS spectra of the composite photocatalysts Bi2WO6/Ag2S. Figure S3. Nitrogen adsorption-desorption isotherms and the pore size distribution curve (inset) of sample (a) Bi2WO6 and (b) Bi2WO6/Ag2S. Figure S4. XRD pattern of Ag2S. Figure S5. Zeta potential for a suspension containing 1 g L of sample Bi2WO6/Ag2S in the presence of KCl (10−3 M) at different pH values. Figure S6. The temporal evolution of Rh B absorption spectra over Bi2WO6/Ag2S heterostructure at different pH values