SURFACE FUNCTIONALIZATION VIA PHOTOINITIATED RADICAL POLYMERIZATION FOR RARE CELL ISOLATION AND MECHANICAL PROTECTION

Surface functionalization of living cells for cell therapeutics has gained substantial momentum in the last two decades. From encapsulating islets of Langerhans, to cell laden gels for tissue scaffolds, to individual cell encapsulation in thin hydrogels, to surface adhesives and inert surface camouflage, modification of living cell surfaces has a wide array of important applications. Here we use hydrogel encapsulation of individual cells as a mode of protection from mechanical forces for high throughput cell printing, and chemical stimuli for the isolation of rare cells in blood. In the first study, we review methods of surface functionalization and establish a metric of potential target biomarkers for circulating tumor cell (CTC) isolation. For extended applications in cancer detection through a fluid biopsy, common surface antigen densities were quantitatively assessed in relation to peripheral blood mononuclear cells (PBMCs) for potential targets of cell specific encapsulation. We then look to commercialization of our process after considering biopsy volumes and cell therapy dose sizes. Undesired batch-to-batch variation in our in-house synthesized photo-initiator could be eliminated by the use of fluorescein, a commercial fluorochrome of similar initiating power to our current eosin initiating system. Fluorescence and hydrogel generation were compared indicating a fluorescein conjugate has comparable power to that of our in-house conjugated eosin. Parameters involving the number of cells and fluid volumes processed were then analyzed systematically. Key parameters were studied to determine optimal equipment and protocol for clinically relevant batch sizes. The final study looks at the mechanical protection provided by thin hydrogel encapsulation. With growing interests in 3D bioprinting and goals of viable whole organ printing for transplant, high resolution and high throughput printing is a growing need. 3D bioprinting presents intense mechanical stimuli in the process that cells must endure. Here we analyze how hydrogel encapsulation reinforces the cellular membrane allowing cells to withstand the damaging forces associated with bioprinting

Office of Global Business: Sustainable Business Global Lab

STEP Category: Education AbroadThe Ohio State University Second-year Transformational Experience Program (STEP)Academic Major: Accountin

Economic aspects of the steel warehouse industry

Thesis (M.B.A)--Boston Universit

What is the impact of faculty learning from and with colleagues? 2008-2009

The professional literature suggests five levels of evaluation for faculty development programs. FDP resource session participation was tracked through registration and attendance. Following the sessions participants were provided with a link to anonymous online evaluations with six Likert-scale items and three open-ended questions. Total attendance at 2008-2009 resource sessions exceed 875

What is the impact of faculty learning from and with colleagues? 2010-2011

The professional literature suggests five levels of evaluation for faculty development programs. FDP resource session participation was tracked through registration and attendance. Following the sessions participants were provided with a link to anonymous online evaluations with six Likert-scale items and three open-ended questions. A brief year-end survey was also sent to all participants in the 2010 resource sessions to further assess the systemic impact of the year’s programs

Can you share the story behind the Nativity scene that is set up in front of Main Hall every holiday season?

Abbot Pennings answers a question about the nativity in front of Main Hall at Christmas time, archived from the SNC website

What is the impact of faculty learning from and with colleagues? 2009-2010

The Faculty Development Program seeks to frame a campus "teaching commons," what the Carnegie Foundation describes as a "conceptual space in which communities of educators committed to innovation and inquiry come together to exchange ideas about teaching and learning, and use them to meet the challenges of educating students for personal, professional, and civic life." Our efforts build on past faculty development traditions at NAU; support departmental, school, and college initiatives; and seek to provide pathways for the NAU learning-centered priority and strategic goals through alignment of initiative

Assessing the scope, role, & impact of the new directions in diversity symposium: Portraits of engagement, 2007-2010

The New Directions in Diversity Symposium seeks to contribute to the campus “teaching commons,” what the Carnegie Foundation describes as a “conceptual space in which communities of educators committed to inquiry and innovation come together to exchange ideas about teaching and learning and use them to meet the challenges of educating students for personal, professional, and civic life.” Our efforts build on the feedback and experiences of each preceding year; support the University’s core values, specifically to “achieve multicultural understanding as a priority of educational and civic life”; guided by the university’s strategic goals, specifically Goal 5, “Inclusion, Civility, and Respect.” The purpose of the Symposium is to engage faculty in an interdisciplinary forum that will ultimately have an impact on the teaching and scholarship faculty pursue