Serum and peritoneal fluid levels of ischemia modified albumin in moderate/severe endometriosis

Background: Recently, the role of oxidative stress in progression of endometriosis has been reported. Ischemia-modified albumin (IMA) is a marker of protein oxidation and very limited number of studies has evaluated the role of IMA in endometriosis. This study was designed to evaluate the serum and peritoneal fluid IMA levels in moderate/severe endometriosis as a marker for oxidative stress.Methods: This study was designed as a prospective controlled clinical trial. The study group consisted of 35 cases who underwent laparoscopy and with a diagnosis of moderate/severe endometriosis. The control group (n=35) was cases without endometriosis that underwent laparoscopy for tubal sterilization. The serum and peritoneal fluid IMA levels were measured spectrophotometrically by colorimetric method with complex of albumin non-binding cobalt and dithioerthreitol.Results: Although the median serum IMA levels in study and control groups were similar (p=0.553), the levels of peritoneal fluid IMA were significantly higher in study group (p=0.044). In endometriosis cases with dysmenorrhea peritoneal fluid IMA levels were much higher than cases without dysmenorrhea (p=0.018).Conclusions: The increased levels of IMA in peritoneal fluid of endometriosis support the possible role of oxidative stress in endometriosis. With this study, peritoneal fluid IMA levels are initially documented in endometriosis cases

Intrafollicular and Circulating Concentrations of Leptin Do Not Predict the Outcome in IVF-ICSI Cycles

Leptin is involved not only in the regulation of food intake but also in other functions including reproduction. Because leptin has been demonstrated to influence ovarian steroidogenesis directly and leptin levels vary during the menstrual cycle and in stimulated cycles, we tested the hypothesis that serum or intrafollicular concentrations of leptin would correlate with reproductive outcomes in intracytoplasmic sperm injection cycles. Serum and follicular fluid samples were collected from 77 women undergoing ovarian stimulation, intracytoplasmic sperm injection and embryo transfer due to male factor infertility. The concentrations of total leptin, both in serum and in pooled follicular fluid samples, did not correlate with the number of oocytes, the fertilization rate or the embryo quality. Additionally, leptin concentrations did not differ between cycles that resulted in pregnancy and those that failed. These results raise objections to the prognostic value of leptin for the outcome of in vitro fertilization/intracytoplasmic sperm injection cycles

Controlled ovarian hyperstimulation with sequential letrozole co-treatment in normo/high responders

Objective: To investigate the effect of co-administration of letrozole in an ovarian stimulation protocol using recombinant FSH and GnRH antagonists for ICSI in normo/high responders

Evaluation of visceral adiposity index with cardiovascular risk factors, biomarkers in postmenopausal women to predict cardiovascular disease: A 10 year study

Background and aim: Visceral adiposity index (VAI) is reportedly beneficial in predicting cardiovascular disease (CVD) and metabolic syndrome (MetS). However, long-term studies analyzing the efficacy of VAI in the pre-diction of CVD risk are limited. The relationship between VAI and electrolytes is unclear. This study aimed to determine if VAI can be used as a predictor of CVD and provide early diagnosis possibility for future CVD pa-tients. Moreover, the impact of biomarkers and electrolytes on VAI therefore indirect relation to CVD was analyzed.Methods: Postmenopausal women (aged >40 years) admitted to our hospital in 2011 were included and cate-gorized into two groups according to their VAI scores: mild/moderate and severe. Groups were compared with insulin resistance, biochemical parameters, and anthropometric measurements. Patients have been reached out after 10 years and questioned for additional disease and cardiovascular risk. Statistical Package for Social Sci-ences (SPSSv22.0) was used for data analysis. The p < 0.05 value was considered significant.Results: Mean VAI score of patients with MetS (7.30 +/- 4.75) was significantly higher than without MetS (2.95 +/- 1.05) (p < 0.01). Serum magnesium level was found significantly lower in the severe group. Serum zinc (Zn) and hsCRP levels were higher in the severe group. Correlation analysis showed significant positive correlations be-tween VAI scores and total cholesterol (r = 0.289, p < 0.05), Zn (r = 0.397, p < 0.01), fasting insulin (r = 0.455, p < 0.01) and no significant association with the 10-year CVD incidence (OR: 1.034 (0.888-1.203); p = 0.668).Conclusion: Previous VAI results cannot assist in predicting the 10-year CVD risk. Additionally, including mea-surements of serum Zn, total cholesterol, fasting insulin, and FBG levels are reasonable approach for managing postmenopausal women with unfavorable CVD risk profiles

Necessity of Salpengectomy for Prevention of Serous Ovarian Tumors

Different theories regarding the origin of serous epithelial tumors of ovary in recent years have been introduced. Fallopian tube especially the fimbriated distal portion is blamed for advancing epithelial carcinomas with transfer of malignant cells over the surface of ovary. The finding of 15% incidence of intraepithelial neoplasias in fallopian tubes removed prophylactically in patients with BRCA mutations, as well as the 70% involvement of tubes in high grade serous tumors support the possible tubal origin of serous ovarian cancers. Here the report of two tubal intraepithelial neoplasia cases detected in which hsyterectomy was performed for benign indications support the preventive function of salpengectomy in future serous epithelial ovarian cancers and alerts the pathologists for detailed examination of distal portion of tubes

The Dietary, Serum and Urine Analysis of Boron and Micronutrients in Postmenopausal Women

Objective: Boron is a nutritionally important trace element that interacts with other micronutrients. Boron plays a critical role in bone mineralization and metabolism. In the present study, the association between boron and micronutrients related to bone metabolism was analysed in postmenopausal women. Methods: In a prospective cohort study in 40 postmenopausal women 24-hour urine and blood samples were collected for sodium, phosphorus, calcium, magnesium, and boron. Daily food consumption, bone mineral density, and Fracture risk assessment tool scores were recorded. Results: The mean age was 53.2 +/- 5.9 years. Dietary habits revealed insufficient dietary fiber and excessive dietary sodium. The serum and urine boron levels were 26.80 mu g/L and 21.22 mu g/day, respectively. Urine boron levels were lower in the osteoporosis group (p = 0.66). A negative correlation between urine Na and boron was detected (p < 0.001). Urinary Na and Ca are negatively correlated with Fracture risk assessment tool scores (p = 0.010, p = 0.019, respectively). Conclusion: The low urinary boron levels in our participants might be due to increased Na excretion due to excessive consumption of Na. Therefore, consulting postmenopausal women about their dietary habits is of concern. Further understanding of the role of boron in bone metabolism will help to accomplish new treatment strategies for osteoporosis and standardization of boron supplementation

Follicular-fluid anti-Mullerian hormone concentrations are predictive of assisted reproduction outcome in PCOS patients

Serum anti-Mullerian hormone (AM H) concentrations constitute a sensitive marker for ovarian ageing. In addition, concentrations of AMH in the follicular fluid constitute a useful marker of embryo implantation in assisted reproduction cycles. The present Study measured serum and follicular-fluid AMH concentrations on the day of oocyte retrieval. These data showed that clinical pregnancy rates (25.0, 34.1 and 42.1%, respectively, P < 0.001). embryo implantation rates (24.3, 35.0 and 44.4%, respectively, P<0.001) and fertilization rates (59.2, 70.9 and 79.5%, respectively, P < 0.001) were markedly different among the low, moderate and high follicular-fluid AMH groups but not among the different serum AMH concentration groups. Follicular-fluid AMH concentrations were negatively correlated with follicular-fluid oestradiol concentrations. The results of this study suggest that follicular-fluid AMH concentration on the day of oocyte retrieval would appear to better reflect the reproductive outcome in PCOS patients undergoing assisted reproduction

Ischaemia-modified albumin in preeclampsia: A critical view

The aim of the study was to compare ischaemia-modified albumin (IMA) levels, both original and corrected, in healthy pregnancies and pregnancies complicated with preeclampsia. Maternal and cord blood samples from study (n = 16) and control (n = 17) groups were collected at the time of delivery. IMA levels were given in absorbance units (ABSU). IMA levels, both original and corrected, were compared between study and control groups. No significant difference was found between maternal and cord blood IMA levels between the study and control groups [1.0 (0.3–3.5) vs 1.2 (0.2–1.4) ABSU, p = .053 and 1.0 (0.1–2.2) vs 0.9 (0.4–3.6) ABSU, p = .382, respectively]. The results were similar for maternal IMA levels, after correction of IMA levels [1.1 (0.3–5.1) vs 1.2 (0.2–1.6) ABSU, p = .292]. IMA is a novel marker for ischaemia, without precise conclusions about its value in preeclampsia. An absolute correction formula, considering all possible intervening factors, is required for more accurate results

The impact of nuchal cord on umbilical cord blood gas analysis and ischaemia-modified albumin levels in elective C-section

This study was designed to evaluate umbilical cord ischaemia-modified albumin (IMA) levels and the cord blood gas parameters of foetuses with or without nuchal cords, at the time of elective C-section. The cross-sectional study population consisted of the patients who were admitted to the Tertiary Care Center between February and June 2015. Women with uncomplicated single term gestations between 37 and 40 completed weeks and scheduled for elective C-sections were included in the study. Fifty cases with a nuchal cord and 50 cases without a nuchal cord were recruited. Nuchal cord blood gas analysis and the IMA levels were evaluated. The IMA levels in umbilical artery of foetuses both in the study and control groups were similar (0.714 +/- 0.150 vs. 0.689 +/- 0.107 ABSU, p = .340, respectively). The umbilical artery pH values of the study group were significantly lower than that in the control group (7.31 +/- 0.04 vs. 7.32 +/- 0.03, p = .042; respectively). The results of the current study indicate that the nuchal cord has an impact on the foetal cord blood gas parameters to some extent before the initiation of labour. Fortunately, this impact does not end up with foetal tissue ischaemia, as confirmed by the IMA levels