Breast Implant Texturization Does Not Affect the Crosstalk Between MSC and ALCL Cells

In the last decade, there has been a growing interest about the possible association between anaplastic large cell lymphoma (ALCL) and breast implants (BIA-ALCL). Many variables, such as breast implants texturization, have been investigated. Breast implants often lead to the formation of a periprosthetic capsule, characterized by inflammation. The presence of the inflamed capsule has been found in the majority of patients with BIA-ALCL. Inflammation may be sustained or counteracted by mesenchymal stem cells (MSCs) by the secretion of pro- or anti-inflammatory cytokines. MSCs were isolated from three capsules surrounding micro-textured (micro-MSCs) and from three capsules surrounding macro-textured (macro-MSCs) implants; after characterization, MSCs were co-cultured with KI-JK cells (a cell line derived from the cutaneous form of ALCL). The secretion of cytokines related to inflammation, the proliferation rate, and the expression of genes referred to pro-tumoral mechanisms were evaluated. Co-cultures of KI-JK cells with micro- or macro-MSCs gave the same results about the secretion of cytokines (increase of IL10, G-CSF, and TGF-\u3b21 and decrease of IL4, IL5, IL12, IL13, IL17A, IFN-\u3b3 (p\u2009<\u20090.05) with respect to mock sample), expression of selected genes (increase for ACVR1, VEGF, TGF-\u3b2R2, CXCL12, and MKi67 (p\u2009<\u20090.05) with respect to control sample), and the proliferation rate (no variation between mock and co-cultured samples). Our results suggest that MSCs derived from capsules surrounding micro- and macro-textured implants display the same effects on the ALCL cells

Breast Implant Texturization Does Not Affect the Crosstalk Between MSC and ALCL Cells

In the last decade, there has been a growing interest about the possible association between anaplastic large cell lymphoma (ALCL) and breast implants (BIA-ALCL). Many variables, such as breast implants texturization, have been investigated. Breast implants often lead to the formation of a periprosthetic capsule, characterized by inflammation. The presence of the inflamed capsule has been found in the majority of patients with BIA-ALCL. Inflammation may be sustained or counteracted by mesenchymal stem cells (MSCs) by the secretion of pro- or anti-inflammatory cytokines. MSCs were isolated from three capsules surrounding micro-textured (micro-MSCs) and from three capsules surrounding macro-textured (macro-MSCs) implants; after characterization, MSCs were co-cultured with KI-JK cells (a cell line derived from the cutaneous form of ALCL). The secretion of cytokines related to inflammation, the proliferation rate, and the expression of genes referred to pro-tumoral mechanisms were evaluated. Co-cultures of KI-JK cells with micro- or macro-MSCs gave the same results about the secretion of cytokines (increase of IL10, G-CSF, and TGF-β1 and decrease of IL4, IL5, IL12, IL13, IL17A, IFN-γ (p < 0.05) with respect to mock sample), expression of selected genes (increase for ACVR1, VEGF, TGF-βR2, CXCL12, and MKi67 (p < 0.05) with respect to control sample), and the proliferation rate (no variation between mock and co-cultured samples). Our results suggest that MSCs derived from capsules surrounding micro- and macro-textured implants display the same effects on the ALCL cells

Mesenchymal stem cell profile in actinic keratosis and its modification after topical application of ingenol mebutate

Mesenchymal stem cells (MSCs) play a crucial role in development of squamous cell carcinoma (SCC)1 , which is considered the opposite pole of a continuous disease spectrum beginning with actinic keratosis (AK)2

Can the extract of Aristolochia argentina Griseb affect the foraging decisions of the leaf cutting ant Acromyrmex lundi (Guérin)? Preliminary assays

The aim of this study was to evaluate whether foraging decisions of Acromyrmex lundi in the field are affected by the extract of Aristolochia argentina. A free choice test was performed using Rosa x hybrida leaves treated with the extract and control with acetone. Two leaves (treated and control) were presented on either side of a foraging trail. The percentage of removed material was recorded for 90 minutes of observation on two consecutive days. Dry weight and repellency index were calculated. Ant activity, the percentage of carried leaves and weight data were analyzed using a “t”-test for paired data, and a General Linear Mixed Model test was used to evaluate the different variables and their interactions. A. argentina extract (1%) did not affect the foraging activity of A. lundi, whereas the 5% dose caused significant differences in foraging activity as well as between the factors and the interactions. A repellency index of above 95% was obtained at the 5% dose. The extract of A. argentina could be considered for future management of this insect.Fil: Defagó, María Teresa. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; ArgentinaFil: Nolli, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentina. Universidad Católica de Córdoba; ArgentinaFil: Diaz Napal, Georgina Natalia. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palacios, Sara Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Córdoba; ArgentinaFil: Buffa, Liliana María. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentin