"Pockett blotting" : a method for transferring nucleic acids onto nylon membranes

We have developed a fast and efficient method for transferring nucleic acids onto nylon membranes. This method requires less DNA for transfer; no decrease in efficiency is observed after successive probing, and several gels can be processed simultaneously. We believe that this technique is of general interest in routine analysis of multiple samples in population genetic studies or in diagnosis purposes. (Résumé d'auteur

Development of improved inhibitors of wall teichoic acid biosynthesis with potent activity against Staphylococcus aureus

A small molecule (1835F03) that inhibits Staphylococcus aureus wall teichoic acid biosynthesis, a proposed antibiotic target, has been discovered. Rapid, parallel, solution-phase synthesis was employed to generate a focused library of analogs, providing detailed information about structure–activity relationships and leading to the identification of targocil, a potent antibiotic

Glutamate transporter EAAT2: a new target for the treatment of neurodegenerative diseases

Glutamate is the primary excitatory amino acid neurotransmitter in the CNS. The concentration of glutamate in the synaptic cleft is tightly controlled by interplay between glutamate release and glutamate clearance. Abnormal glutamate release and/or dysfunction of glutamate clearance can cause overstimulation of glutamate receptors and result in neuronal injury known as excitotoxicity. The glial glutamate transporter EAAT2 plays a major role in glutamate clearance. Dysfunction or reduced expression of EAAT2 has been documented in many neurodegenerative diseases. In addition, many studies in animal models of disease indicate that increased EAAT2 expression provides neuronal protection. Here, we summarize these studies and suggest that EAAT2 is a potential target for the prevention of excitotoxicity. EAAT2 can be upregulated by transcriptional or translational activation. We discuss current progress in the search for EAAT2 activators, which is a promising direction for the treatment of neurodegenerative diseases

Y-aminobutyric acid type B (GABAB) receptor expression is needed for inhibition of N-type (Cav2.2) calcium channels by analgesic a-conotoxins

Background: A class of analgesic a-conotoxins potently inhibits N-type calcium channels. Results: The activity of a-conotoxins Vc1.1 and RgIA was reduced following knockdown of GABAB receptor expression in sensory neurons and can be reconstituted in HEK293 cells expressing human GABAB receptor and Cav2.2. Conclusion: GABAB receptors are needed for inhibition of Cav2.2 by Vc1.1 and RgIA. Significance: These analgesic a-conotoxins activate human GABAB receptors

The retroflection of part of the East Greenland Current at Cape Farewell

The East Greenland Current (EGC) and the smaller East Greenland Coastal Current (EGCC) provide the major conduit for cold fresh polar water to enter the lower latitudes of the North Atlantic. They flow equatorward through the western Irminger Basin and around Cape Farewell into the Labrador Sea. The surface circulation and transport of the Cape Farewell boundary current region in summer 2005 is described. The EGCC merges with Arctic waters of the EGC to the south of Cape Farewell, forming the West Greenland Current. The EGC transport decreases from 15.5 Sv south of Cape Farewell to 11.7 Sv in the eastern Labrador Sea (where the water becomes known as Irminger Sea Water). The decrease in EGC transport is balanced by the retroflection of a substantial proportion of the boundary current (5.1 Sv) into the central Irminger Basin; a new pathway for fresh water into the interior of the subpolar gyre

The Centrality of the Center: Best Practices for Engaging Students on Campus

Communication centers exist primarily as a complementary student service (Strawser, Apostel, Carpenter, Cuny, Dvorak, & Head, 2019). As an integral campus student services, centers must place an overarching emphasis on student engagement. Student engagement, according to NSSE, is the time and effort students put into their educational activities and the institutional deployment of educational resources. Communication centers, to continue to prove their value to institutions, must continue to build programming and initiatives that are worthy of students’ time and get students to participate. To address engagement concerns, the authors of this essay offer ten best practices for building and sustaining student engagement in the communication center. The best practices are universal and transferable, meaning, any center, no matter the vision or the resources, could theoretically implement the ideas

Міхал Бобжинський: суспільно-політична діяльність

Розглянута суспільно-політична діяльність визначного представника краківської історичної школи М. Бобжинського. Акцентовано увагу на зв’язку наукових поглядів історика з його політичною практикою. Охарактеризовано дискусії, які досьогодні точаться серед науковців щодо оцінки результатів його діяльності.Рассмотрена общественно-политическая деятельность выдающегося представителя краковской исторической школы М. Бобжинского. Акцентировано внимание на связи научных взглядов историка с его политической практикой. Охарактеризованы дискуссии, которые досегодня ведутся среди ученых относительно оценки результатов его деятельности.The socio-political activities of M. Bobzhynski – a prominent representative of the Krakow historical school – are reviewed. Attention is given to the connection of the historian scholarly views with his political practice. The discussions that until now are going on among scholars in evaluating the results of his work are characterised