13 research outputs found
THE RELATIONSHIP STUDY OF TEACHER’S PERCEPTION ON THEIR LEADERSHIP CAPACITY AND TEACHING COMPETENCE AT BAOSHAN EXPERIMENTAL SCHOOL, SHANGHAI, CHINA
The primary purpose of this research study was to determine the relationship between teachers' perceptions of their leadership capacity and competence at Baoshan Experimental School, Shanghai, China. The research instrument was a questionnaire for investigating the teachers' perceptions of their leadership capacity and competence adopted from the previous studies. A total of 120 teachers who were full-time teachers working at the school participated in this study during the academic year of 2020.Mean and Standard Deviation were used to identify teachers' perceptions of their leadership capacity and determine teachers' level of competence. Then, Pearson's Product Moment Correlation Coefficient was then used to determine the significant relationship between teachers' perceptions of leadership capacity and their competence.The findings showed that teachers' perceptions of their leadership capacity and professional competence were high. Moreover, the correlation analysis result showed a strong positive relationship between teachers' perceptions of their leadership capacity and competence at Baoshan Experimental School, Shanghai, China
Analysis of differential membrane proteins related to matrix stiffness-mediated metformin resistance in hepatocellular carcinoma cells
Abstract Background Our previous work shows that increased matrix stiffness not only alters malignant characteristics of hepatocellular carcinoma (HCC) cells, but also attenuates metformin efficacy in treating HCC cells. Here, we identified differential membrane proteins related to matrix stiffness-mediated metformin resistance for better understand therapeutic resistance of metformin in HCC. Methods Differential membrane proteins in HCC cells grown on different stiffness substrates before and after metformin intervention were screened and identified using isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with the liquid chromatography-tandem mass spectrometry (LC–MS/MS), then bioinformatic analysis were applied to determine candidate membrane protein and their possible signaling pathway. Results A total of 5159 proteins were identified and 354 differential membrane proteins and membrane associated proteins, which might be associated with matrix stiffness-mediated metformin resistance were discovered. Then 94 candidate membrane proteins including 21 up-regulated protein molecules and 73 down-regulated protein molecules were further obtained. Some of them such as Annexin A2 (ANXA2), Filamin-A (FLNA), Moesin (MSN), Myosin-9 (MYH9), Elongation factor 2 (eEF2), and Tax1 binding Protein 3 (TAX1BP3) were selected for further validation. Their expressions were all downregulated in HCC cells grown on different stiffness substrates after metformin intervention. More importantly, the degree of decrease was obviously weakened on the higher stiffness substrate compared with that on the lower stiffness substrate, indicating that these candidate membrane proteins might contribute to matrix stiffness-mediated metformin resistance in HCC. Conclusions There was an obvious change in membrane proteins in matrix stiffness-mediated metformin resistance in HCC cells. Six candidate membrane proteins may reflect the response of HCC cells under high stiffness stimulation to metformin intervention, which deserve to be investigated in the future
Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker
Gastrodia elata (G. elata) tuber is a valuable herbal medicine used to treat many diseases. The procedure of establishing a reasonable and feasible quality assessment method for G. elata tuber is important to ensure its clinical safety and efficacy. In this research, an effective and comprehensive evaluation method for assessing the quality of G. elata has been developed, based on the analysis of high performance liquid chromatography (HPLC) fingerprint, combined with the quantitative analysis of multi-components by single marker (QAMS) method. The contents of the seven components, including gastrodin, p-hydroxybenzyl alcohol, p-hydroxy benzaldehyde, parishin A, parishin B, parishin C, and parishin E were determined, simultaneously, using gastrodin as the reference standard. The results demonstrated that there was no significant difference between the QAMS method and the traditional external standard method (ESM) (p > 0.05, RSD < 4.79%), suggesting that QAMS was a reliable and convenient method for the content determination of multiple components, especially when there is a shortage of reference substances. In conclusion, this strategy could be beneficial for simplifying the processes in the quality control of G. elata tuber and giving references to promote the quality standards of herbal medicines
Additional file 3 of Analysis of differential membrane proteins related to matrix stiffness-mediated metformin resistance in hepatocellular carcinoma cells
Additional file 3:Â Table S2. A list of all identified proteins
Additional file 2 of Analysis of differential membrane proteins related to matrix stiffness-mediated metformin resistance in hepatocellular carcinoma cells
Additional file 2:Â Figure S1. Efficiency and quality identification of membrane protein extraction. (A) The approximate location and range of membrane protein molecules performed by polyacrylamide gel electrophoresis with Coomassie brilliant blue staining. (B)(i, ii) Western blot were performed on the six groups of membrane proteins and cytoplasmic proteins
Additional file 1 of Analysis of differential membrane proteins related to matrix stiffness-mediated metformin resistance in hepatocellular carcinoma cells
Additional file 1:Â Table S1. Ingredients of polyacrylamide gel substrates with variable stiffness
Additional file 6 of Analysis of differential membrane proteins related to matrix stiffness-mediated metformin resistance in hepatocellular carcinoma cells
Additional file 6:Â Figure S3. Six typical expression patterns reflect the decrease degree of membrane proteins. iTRAQ tags 113, 114, 115, 116, 117 and 118 represent NC-L, NC-M, NC-H, Met-L, Met-M and Met-H, respectively
Additional file 4 of Analysis of differential membrane proteins related to matrix stiffness-mediated metformin resistance in hepatocellular carcinoma cells
Additional file 4:Â Table S3. Differential membrane proteins and membrane associated proteins
Additional file 5 of Analysis of differential membrane proteins related to matrix stiffness-mediated metformin resistance in hepatocellular carcinoma cells
Additional file 5:Â Figure S2. Six typical expression patterns reflect the increase degree of membrane proteins. iTRAQ tags 113, 114, 115, 116, 117 and 118 represent NC-L, NC-M, NC-H, Met-L, Met-M and Met-H, respectively