Mesenchymal Stem Cells Induce T-Cell Tolerance and Protect the Preterm Brain after Global Hypoxia-Ischemia

Hypoxic-ischemic encephalopathy (HIE) in preterm infants is a severe disease for which no curative treatment is available. Cerebral inflammation and invasion of activated peripheral immune cells have been shown to play a pivotal role in the etiology of white matter injury, which is the clinical hallmark of HIE in preterm infants. The objective of this study was to assess the neuroprotective and anti-inflammatory effects of intravenously delivered mesenchymal stem cells (MSC) in an ovine model of HIE. In this translational animal model, global hypoxia-ischemia (HI) was induced in instrumented preterm sheep by transient umbilical cord occlusion, which closely mimics the clinical insult. Intravenous administration of 2 x 106MSC/kg reduced microglial proliferation, diminished loss of oligodendrocytes and reduced demyelination, as determined by histology and Diffusion Tensor Imaging (DTI), in the preterm brain after global HI. These anti-inflammatory and neuroprotective effects of MSC were paralleled by reduced electrographic seizure activity in the ischemic preterm brain. Furthermore, we showed that MSC induced persistent peripheral T-cell tolerance in vivo and reduced invasion of T-cells into the preterm brain following global HI. These findings show in a preclinical animal model that intravenously administered MSC reduced cerebral inflammation, protected against white matter injury and established functional improvement in the preterm brain following global HI. Moreover, we provide evidence that induction of T-cell tolerance by MSC might play an important role in the neuroprotective effects of MSC in HIE. This is the first study to describe a marked neuroprotective effect of MSC in a translational animal model of HIE

Ett samtal om samtal : Chefers upplevelse av medarbetarsamtalet som förändringsverktyg

Studien syftar till att undersöka medarbetarsamtal utifrån ett chefsperspektiv och studien intresserar sig för vilka effekter cheferna upplever att samtalet generar i verksamheten och hur de använder sig av samtalet i utveckling av verksamheten. Metoden är kvalitativ och utgörs av åtta stycken semistrukturerade intervjuer, där individer med personalansvar fick möjlighet att berätta sina upplevelser och erfarenheter av samtalet samt reflektera runt dess betydelse utifrån deras position i organisationen. Resultatet visar att medarbetarsamtalet är en tids- och energikrävande insats för cheferna som ibland måste ställa sin fritid till förfogande. Dialog och social kompetens lyfts fram som nyckelfaktorer för ett bra samtal och deltagarna i studien förlitar sig på sin upparbetade erfarenhet och känner en trygghet i sin yrkesroll vid genomförandet av samtalet. Denna studie bidrar till djupare kunskap om chefernas upplevelse av medarbetarsamtalet och dess potential som styrverktyg inom organisationer och kan medverka till framtida forskning som ett komplement till studier rörande medarbetarnas upplevelse. Studien visar att det även kan vara av intresse att via observationer av samtalet undersöka hur väl det praktiska genomförandet stämmer överens med chefens intentioner om verksamhetsutveckling.This study aims to examine employee review, based on a manager perspective with an interest in what effects leaders' experience it is generating, and how they use the employee review in the development of their organization. The method used in this study is qualitative and comprises eight semi-structured interviews, in which individuals with personnel responsibility where given the opportunity to share their comprehension off and experience from the phenomenon employee review, and its importance based on their position in the organization. The result shows that the employee review is a time and energy-intensive effort for the managers who sometimes have to make their spare time available for the task.Dialogue and social skills are highlighted as key factors for a good conversation, and the participants in the study relies on their self-generated experience and feel confident in their professional role in the implementation of employee review. This study contributes to deeper knowledge of the managers' experience regarding the employee review and its potential as an organizational management tool, and can contribute to future research as a complement to studies regarding the employees' experience. The results shows that it may also be of interest to study employee reviews through observations of the interview, how well the practical implementation is in line with the manager's intentions on business development