A soft method for the production of CdSe and CdSe/ZnS quantum dots

Comunicação Oral Convidada.A soft method for the production of CdSe and CdSe/Zns quantum dots is presented, as well as the main experimental results.Fundação para a Ciência e a Tecnologia (FCT), FEDER and QREN for financial support to CFUM [Strategic Project PEst-C/FIS/UI0607/2011 (F-COMP-01-0124-FEDER-022711)] and to the research project PTDC/FIS/113199/2009 also financed by COMPETE/QREN/EU

Efeito de defensivos sobre Anoplosiagum sp. (coleoptera: scarabaeidae).

JIPE 2013

Gold or silver nanoparticles decorating superparamagnetic calcium ferrite nanoparticles for application in SERS

In this work, we report the synthesis of Ag and Au nanoparticles capped with either cysteamine or cysteine. Purification of these NPs turned out to originate irreversible aggregation, so that the coupling of NH2 groups from Au (or Ag) nanoparticles to OH groups of magnetic calcium ferrite (CaFe2O4) nanoparticles (MNPs) would not work as expected. Therefore, naked metallic Ag or Au nanoparticles were produced and connection to calcium ferrite was performed by first attaching cysteine to the MNPs and then using the resulting SH pending groups to interact with the metallic surface.Financial support by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding of CF-UM-UP (UID/FIS/04650/2013).info:eu-repo/semantics/publishedVersio

Current practices in managing acutely disturbed patients at three hospitals in Rio de Janeiro-Brazil: a prevalence study

The medical management of aggressive and violent behaviour is a critical situation for which there is little evidence. In order to prepare for a randomised trial, due to start in the psychiatric emergency rooms of Rio de Janeiro in 2001, a survey of current practice was necessary. A seven day survey of pharmacological management of aggressive people with psychosis in the emergency rooms of all four public psychiatric hospitals in Rio de Janeiro, Brazil. In one hospital data were not available. Of the 764 people with psychosis attending these ERs, 74 were given IM medication for rapid tranquillisation (9.7%, 2.1/week/100,000). A haloperidol-promethazine mix (with or without other drugs) was used for the majority of patients (83%). The haloperidol-promethazine mix, given intramuscularly for rapid tranquilization, is prevalent in Rio, where it is considered both safe and efficient. However, scientific evaluation of all pharmacological approaches to rapid tranquilization of psychotic people is inadequate or incomplete and a randomized trial of IM haloperidol-promethazine is overdue

Bioindicators as a tool for monitoring and control biofilm reactors : a simplified approach

The control of wastewater treatment plants requires an extensive monitoring programme based on physicochemical costly routine analysis. Microbial populations are well known indicators of operational conditions in biological reactors. This research was carried out by the CENTA and the University of Minho in the scope of the Erasmus programme. The aim of the work was the development of a simplified approach, based on biological indicators, for monitoring small biofilm wastewater treatment processes. In that regard, protozoa and metazoan were monitored at CENTA experimental plants - namely a trickling filter and a rotating biological contactor -, and correlated with influent wastewater and effluent composition. The relationship between wastewater performance and the microbiological composition of the biofilm was identified and assessed. Results indicate that a methodology based on the bioindicators provides useful data for process monitoring and control of small wastewater treatment plants, thus diminishing the associated costs of routine analysis and providing information when such analysis aren’t easily available

Uso combinado de dados NDVI/MODIS dos satélites Terra e Aqua no monitoramento multi-temporal de áreas agrícolas.

O objetivo deste estudo foi analisar a utilização combinada de dados de NDVI do sensor MODIS (Moderate-resolution Imaging Spectroradiometer) provenientes de duas plataformas orbitais - Terra e Aqua - na geração de perfis temporais em áreas agrícolas.SBSR 2013

Análise de ciclos harmônicos de séries temporais de dados NDVI/MODIS para a discriminação de culturas agrícolas anuais.

Este trabalho desenvolveu e testou uma abordagem de representação de ciclos harmônicos de séries temporais de produtos MODIS, para o mapeamento da agricultura no Estado de Mato Grosso, tomando como base a metodologia proposta por Antunes et al. (2012), proposta para o mapeamento da paisagem da bacia do alto Paraguai.SBSR 2013

Superação da insuficiência de frio hibernal em ameixeiras (prunus domestica L.) cv D'agen.

bitstream/item/31592/1/comunicado79.pd

Monoolein-based lipoplexes (DODAB/MO/DNA) as non-viral vector for transfection- from physicochemical characterization to biological application

Cationic liposomes/DNA (lipoplexes) have been widely used as non-viral vectors for transfection, the role of the neutral lipid in liposome formulation being determinant for the efficiency of this process [1,2]. In this work, we studied the potential of monoolein (MO) as helper lipid for cellular transfection. Lipoplexes composed of pDNA and dioctadecyldimethylammonium bromide (DODAB)/1-monooleoyl-rac-glycerol (MO) at different molar ratios (4:1, 2:1 and 1:1) were investigated, as well as different cationic lipid/DNA ratios. The physicochemical properties of the lipoplexes (size and charge), the formation of the lipoplexes, the effect of MO on pDNA condensation and the effect of heparin on percentage of pDNA release from the lipoplexes were also studied by Ethidium Bromide (EtBr) exclusion assays, Dynamic Light Scattering (DLS), Zeta Potential (æ) and electrophoresis. The cytotoxicity, transfection efficiency, as well as the intracellular localization of labeled DNA were evaluated on 293T cells. It was found that the presence of MO not only increases the efficiency of pDNA compactation, but also affects the physicochemical properties of lipoplexes, which could possibly interfere with lipoplex-cell interactions. The DODAB:MO (2:1) and (4:1) formulations were capable of efficiently mediate in vitro cell transfection. These results were consistent with fluorescence microscopy studies, which illustrated that lipoplexes were able to gain entry into the cytosol and deliver pDNA to the nucleus. Understanding the structure–activity relationship of MO based lipoplexes will give direction toward the development of safe and efficient gene delivery systems.Portuguese Foundation for Science and Technology (FCT) for financial support to Center of Physics and Center of Molecular & Environmental Biology and funding through projects PTDC/QUI/69795/2006 and SFRH/BD/46968/2009