Ethylene-regulated gene expression in tomato fruit: characterization of novel ethylene-responsive and ripeningrelated genes isolated by differential display.

Differential display was used to isolate early ethyleneregulated genes from late immature green tomato fruit in order to obtain a broader understanding of the molecular basis by which ethylene coordinates the ripening process. Nineteen novel ethylene-responsive (ER) cDNA clones were isolated that fell into three classes: (i) ethylene up-regulated (ii) ethylene downregulated, and (iii) transiently induced. Expression analysis revealed that ethylene-dependent changes in mRNA accumulation occurred rapidly (15 min) for most of the ER clones. The predicted proteins encoded by the ER genes are putatively involved in processes as diverse as primary metabolism, hormone signalling and stress responses. Although a number of the isolated ER clones correspond to genes already documented in other species, their responsiveness to ethylene is described here for the ®rst time. Among the ER clones sharing high homology with regulatory genes, ER43, a putative GTP-binding protein, and ER50, a CTR1-like clone, are potentially involved in signal transduction. ER24 is homologous to the multiprotein bridging factor MBF1 involved in transcriptional activation, and ®nally, two clones are homologous to genes involved in post-transcriptional regulation: ER49, a putative translational elongation factor, and ER68, a mRNA helicase-like gene. Six ER clones correspond to as yet unidenti®ed genes. The expression studies indicated that all the ER genes are ripening-regulated, and, depending on the clone, show changes in transcript accumulation either at the breaker, turning, or red stage. Analysis of transcript accumulation in different organs indicated a strong bias towards expression in the fruit for many of the clones. The potential roles for some of the ER clone

Changes in gene expression during pod development in oilseed rape

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Apartheid in South African industrial relations, 1955-1980

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Effects of subsurface drip irrigation rates and furrow irrigation for cotton grown on a vertisol on off-site movement of sediments, nutrients and pesticides

Abstract – Subsurface drip irrigation can reduce off-farm movements of fertilizers and pollutants and improve the water use efficiency of irrigated agriculture. Here we compared the effects of furrow and subsurface drip at different irrigation rates, based on a percentage of daily crop-evapotranspiration rates (ETc), on run-off and off-site movement of suspended sediment, nutrients and pesticides from cotton crops grownon a vertisol. Our results show that furrow irrigation significantly increased suspended soil loss, of 5.26 t ha−1, compared to that of subsurface drip irrigation at 120% of ETc, of 2.53 t ha−1, whereas no erosion was recorded with deficit subsurface drip irrigation. Off-site movement of nitrogen in furrow, of 18.63 kg ha−1, was five times greater than subsurface drip irrigation at 120% ETc. It was much less with 105% ETc(0.37 kg ha−1) and 90% ETc (0.15 kg ha−1), and absent for 75% and 50% of ETc. Phosphorus loss from furrow, of 778 g ha−1, was greater than for the wetter subsurface drip treatments that gave 23 g ha−1 for 90% ETc and 19 g ha−1 for 120% ETc. No P loss was recorded from drier subsurface drip irrigation rates. Herbicides such as atrazine and diuron were applied in the year prior to the experiment, but considerable amounts were recorded in furrow run-off in both years, but only at 90 and 120% ETc subsurface drip irrigation in the first year. Concentrations of applied herbicide residues in the runoff exceeded the minimum threshold level for 99% species protection and, although the total amount of herbicide movement was higher in furrow, at times the concentration was greater for wetter subsurface drip irrigation run-off. Residues of insecticides, such as endosulphan applied in a previous year and dimethoate applied in the current years, were recorded in runoff from subsurface drip at 120% and furrow irrigation. Their concentrations in each year exceeded minimum threshold level. Subsurface drip irrigation at 75% ETc offered the best trade-off between off-site run-off, erosion and pesticide movement and yield and water use efficiency

Author Correction: The FLUXNET2015 dataset and the ONEFlux processing pipeline for eddy covariance data (Scientific Data, (2020), 7, 1, (225), 10.1038/s41597-020-0534-3)

The following authors were omitted from the original version of this Data Descriptor: Markus Reichstein and Nicolas Vuichard. Both contributed to the code development and N. Vuichard contributed to the processing of the ERA-Interim data downscaling. Furthermore, the contribution of the co-author Frank Tiedemann was re-evaluated relative to the colleague Corinna Rebmann, both working at the same sites, and based on this re-evaluation a substitution in the co-author list is implemented (with Rebmann replacing Tiedemann). Finally, two affiliations were listed incorrectly and are corrected here (entries 190 and 193). The author list and affiliations have been amended to address these omissions in both the HTML and PDF versions. © 2021, This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply