Regional geochemical and geophysical surveys in the Berwyn Dome and adjacent areas, north Wales

This report describes stream sediment and gravity surveys carried out across the Berwyn Dome and adjacent areas. The gravity survey confirmed the presence of a broad regional Bouguer anomaly low in the central part of the Dome, on which is superimposed several smaller irregular highs and lows. Some of these local anomalies possibly reflect small igneous bodies but more detailed gravity surveys would be needed to determine their form. Near Corwen the Bryneglwys Fault coincides with a 4.5 mGa1 anomaly but southwards the two features diverge, suggesting that the density interface is related either to a splay fault or to the eastern margin of the Lower Palaeozoic Montgomery trough. Some other structural trends are weakly reflected on the Bouguer anomaly and aeromagnetic maps, but there is no clear correlation with known base metal mineralisation. The Bouguer anomalies cannot be attributed to particular structures with any certainty but are probably due to a number of factors, including variation in the Precambrian basement and changes in the lithology and thickness of Lower Palaeozoic sedimentary rocks. There is no evidence for a large granitic body in Lower Palaeozoic rocks underlying the mineralisation at Llangynog. The aeromagnetic map suggests the presence of a magnetic basement at a depth of 3-4 km centred beneath the northwestern margin of the Dome. The stream sediment survey involved the collection of a - 100 mesh stream sediment, panned concentrate and water sample from each of the 399 sites sampled. The sample density was 1 site per 1.5 km*. Cu, Pb, Zn, Ba, Fe, Mn, Co, V, Cr, Ni, Zr, MO and Sn were determined in the stream sediments, Cu, Pb, Zn, Ba, Fe, Mn, Ce, Sn, Sb, Ti, Ni and As in the panned concentrates and Cu, Pb and Zn in stream waters. Major variations in the results are related to (i) hydrous oxide precipitation processes, (ii) contamination from human activities, (iii) base metal and baryte mineralisation, (iv) monazite concentrations in panned concentrates, (v) hitherto unrecorded gold mineralisation and (vi) lithological variations. The latter were related principally to shale-sandstone variation, but groups of elements attributable to the presence of basic intrusions, phosphatic rocks, coal measures, sandstones, limestones and volcanics were also discerned. Threshold levels were established from cumulative frequency curve analysis, and some anomalous sites were examined in the field. Anomalies did not form prominent coherent groups and were generally weak and scattered, with a wide variety of element groupings reflecting a range of causes. Many anomalous panned concentrates were examined mineralogically to try to . determine whether anomalies were related to chemically extreme background lithologies, contamination, or mineralisation. All the anomalies were related to one or more of the major causes of variation, although because of the very limited amount of follow-up work carried out the precise cause of many anomalies remains uncertain. No anomaly is considered to represent a strong prospect but several deserve further limited investigation, notably those associated with (i) gold mineralisation in the northwest of the area, (ii) baryte, perhaps accompanied by base metal ’ mineralisation, associated with Caradocian volcanics and phosphatic rocks at several localities, (iii) mineralisation associated with Llandeilian limestones and volcanic rocks north of Llanrhaeadr, and (iv) copper mineralisation associated with intrusives near the eastern margin of the Dome, where survey data is most incomplete

Exploration for volcanogenic sulphide mineralisation at Benglog, north Wales

Exploration for volcanogenic sulphide mineralisation around Benglog is one of three investigations designed to assess the metallogenic potential of the Ordovician Aran Volcanic Group. Detailed geological mapping in the Benglog area enabled an interpretation of the volcanic environment, critical to such an assessment, to be made. The eruptive rocks are acid and basic in composition; the acid rocks are mostly ash-flow tuffs derived from outside the area, whereas the basic rocks have a local derivation. They are all interbedded with dark grey or black silty mudstone and were probably erupted in a submarine environment. Contemporaneous dolerite sills were intruded into wet sediment. This environment was suitable for volcanogenic exhalative sulphide deposits to form and indications of a metallogenic horizon were found at the top of the Y Fron Formation in the form of abundant pyrite, minor pyrrhotite and minor base metal enrichment. Soil samples, analysed for copper, lead and zinc, were collected and geophysical surveys were carried out along eleven east-west trending traverse lines 300 m apart across the volcanic succession. Indications were found of minor vein mineralisation at dolerite intrusion margins and locally along faults. Very high chargeability and low resistivity anomalies over mudstones did not spatially coincide with geochemical anomalies in soil, but the secondary redistribution of metals in soils and variable thickness of overburden precluded confident interpretation of the source of many soil anomalies. Geochemical drainage data, in conjunction with rock analyses, show strong barium enrichment in mudstones which could be volcanogenic in origin but related to two separate eruptive episodes. The findings of the survey were inconclusive. An environment suitable for the formation of volcanogenic exhalative sulphide deposits was established, but the geochemical and geophysical surveys located only minor vein mineralisation and tenuous indications of other styles of mineralisation. Recommendations are made for further work

Cocaine self-administration in the mouse: A low- cost, chronic catheter preparation

Intravenous drug self-administration is the most valid animal model of human addiction because it allows volitional titration of the drug in the blood based on an individual’s motivational state together with the pharmacokinetic properties of the drug. Here we describe a reliable low-cost mouse self-administration catheter assembly and protocol that that can be used to assess a variety of drugs of abuse with a variety of protocols. We describe a method for intravenous catheter fabrication that allows for efficient and long-lasting intravenous drug delivery. The intravenous catheters remained intact and patent for several weeks allowing us to establish stable maintenance of cocaine acquisition. This was followed by a dose response study in the same mice. For collaborators interested in premade catheters for research please make a request at www.neuro-cloud.net/nature-precedings/pomerenze

Mineral exploration in the Cockermouth area, Cumbria. Part 2: follow-up surveys

This report describes the results of geochemical, geological and geophysical surveys across three small areas of Carboniferous and Lower Palaeozoic rocks along the northern margin of the English Lake District. The areas were chosen from the appraisal of regional-scale survey data described by Cooper et al. (1991). In two of the areas, Ruthwaite and Tallentire, the objective was to provide more information on the extent and magnitude of fracture-controlled epigenetic baryte and base metal mineralisation. In the third area, at Whitrigg, brief surveys were carried out to aid the interpretation of unexplained geochemical and geophysical anomalies found during two projects carried out under the Mineral Exploration and Investment Grants Act (MEIGA). At Ruthwaite, where a mine formerly worked baryte from a fault separating Lower Palaeozoic and Carboniferous rocks, surface indications of further baryte mineralisation were found. Soil analyses indicated that mineralisation may be present along the continuation of the faultline worked at Ruthwaite and in the Eycott Volcanic Group rocks to the south of it. In this area relatively small, but in some circumstances perhaps economically attractive, deposits of baryte may be present under drift cover. In the Tallentire Hill area, geological mapping followed by traverse-based soil sampling showed that fracture-controlled mineralisation is widespread in the Carboniferous (Dinantian and Namurian) rocks. The fracture fillings consist dominantly of baryte, often accompanied by carbonate, with traces of copper and mercury. Where seen at surface the fracture fillings are too small, patchy and low-grade to be of any economic importance. Baryte mineralisation also occurs locally as patchy impregnations in sandstones. These are considered to be epigenetic deposits related to the fracture-controlled mineralisation. Trial geophysical surveys suggested that electrical methods may be useful in determining the extent of the mineralised sandstone. There is a possibility that more extensive baryte deposits may be present in the limestone succession underlying the mineralised sandstones. In the Whitrigg area, Carboniferous rocks are separated from Lower Palaeozoic rocks of the Eycott Volcanic Group by the easterly-trending Boundary Fault and north-westerly-trending Bothel Fault. Evidence from an old mineral working and the results of a soil survey indicate that patchy, epigenetic, fracture-controlled baryte and base metal mineralisation occurs along the Bothel Fault and, locally, in the adjacent rocks. A feature of this mineralisation is the presence of mercury, which is most abundant in a sample of brecciated and altered rock from the Eycott Volcanic Group. Prominent base metal in soil anomalies discovered by MEIGA-funded projects near Stangerhill are not associated with barium anomalies. It was concluded that these soil anomalies are most likely to be caused by secondary concentration in overburden, and that the source of metals may be a sub-cropping metalliferous horizon within the Carboniferous succession or, more probably, fracture-controlled mineralisation. Trial geophysical surveys carried out in all three areas indicated that in ground free of artificial sources the VLF(EM) and conductivity mapping methods could be useful for tracing faults beneath drift and providing information on drift thickness. Closely-spaced soil sampling proved effective for detecting mineralisation in areas where the drift cover is thin, and a trial soil-gas survey showed that this technique could also be useful for tracing faults beneath drift

Disordered Hubbard Model with Attraction: Coupling Energy of Cooper Pairs in Small Clusters

We generalize the Cooper problem to the case of many interacting particles in the vicinity of the Fermi level in the presence of disorder. On the basis of this approach we study numerically the variation of the pair coupling energy in small clusters as a function of disorder. We show that the Cooper pair energy is strongly enhanced by disorder, which at the same time leads to the localization of pairs.Comment: revtex, 5 pages, 6 figure

Haplotyping the human leukocyte antigen system from single chromosomes

We describe a method for determining the parental HLA haplotypes of a single individual without recourse to conventional segregation genetics. Blood samples were cultured to identify and sort chromosome 6 by bivariate flow cytometry. Single chromosome 6 amplification products were confirmed with a single nucleotide polymorphism (SNP) array and verified by deep sequencing to enable assignment of both alleles at the HLA loci, defining the two haplotypes. This study exemplifies a rapid and efficient method of haplotyping that can be applied to any chromosome pair, or indeed all chromosome pairs, using a single sorting operation. The method represents a cost-effective approach to complete phasing of SNPs, which will facilitate a deeper understanding of the links between SNPs, gene regulation and protein function

Onset of magnetism in B2 transition metals aluminides

Ab initio calculation results for the electronic structure of disordered bcc Fe(x)Al(1-x) (0.4<x<0.75), Co(x)Al(1-x) and Ni(x)Al(1-x) (x=0.4; 0.5; 0.6) alloys near the 1:1 stoichiometry, as well as of the ordered B2 (FeAl, CoAl, NiAl) phases with point defects are presented. The calculations were performed using the coherent potential approximation within the Korringa-Kohn-Rostoker method (KKR-CPA) for the disordered case and the tight-binding linear muffin-tin orbital (TB-LMTO) method for the intermetallic compounds. We studied in particular the onset of magnetism in Fe-Al and Co-Al systems as a function of the defect structure. We found the appearance of large local magnetic moments associated with the transition metal (TM) antisite defect in FeAl and CoAl compounds, in agreement with the experimental findings. Moreover, we found that any vacancies on both sublattices enhance the magnetic moments via reducing the charge transfer to a TM atom. Disordered Fe-Al alloys are ferromagnetically ordered for the whole range of composition studied, whereas Co-Al becomes magnetic only for Co concentration >0.5.Comment: 11 pages with 9 embedded postscript figures, to be published in Phys.Rev.

A platform for curated products from novel Open Reading Frames (nORFs) prompts reinterpretation of disease variants

Recent evidence from proteomics and deep massively parallel sequencing studies have revealed that eukaryotic genomes contain substantial numbers of as-yet-uncharacterized open reading frames (ORFs). We define these uncharacterized ORFs as novel ORFs (nORFs). nORFs in humans are mostly under 100 codons and are found in diverse regions of the genome, including in long noncoding RNAs, pseudogenes, 3′ UTRs, 5′ UTRs, and alternative reading frames of canonical protein coding exons. There is therefore a pressing need to evaluate the potential functional importance of these unannotated transcripts and proteins in biological pathways and human disease on a larger scale, rather than one at a time. In this study, we outline the creation of a valuable nORFs data set with experimental evidence of translation for the community, use measures of heritability and selection that reveal signals for functional importance, and show the potential implications for functional interpretation of genetic variants in nORFs. Our results indicate that some variants that were previously classified as being benign or of uncertain significance may have to be reinterpreted