CERTIFICATION REPORT: The certification of different mass fractions of Bt11 in maize powder: Certified Reference Materials: ERM®-BF412ak, ERM®-BF412bk, ERM®-BF412ck, ERM®-BF412dk and ERM®-BF412ek

This report describes the production of a set of Certified Reference Materials (CRMs), ERM-BF412ak, bk, ck, dk and ek, which are certified for their Bt11 maize (unique identifier SYN-BTØ11-1) event mass fractions. These materials were produced following ISO Guide 34:2009 and are certified in accordance with ISO Guide 35:2006. Genetically modified (GM) maize seeds of the Bt11 event and seeds from a non GM maize variety were milled to obtain GM and non-GM seed powders with a similar particle size distribution. Mixtures of non-GM and GM maize seed powder were prepared gravimetrically. The certified values were obtained from the gravimetric preparations, taking into account the water mass fractions of the two powder materials and the genetic purity with respect to the Bt11 maize. The certified values were confirmed by event-specific real-time PCR as an independent verification method (measurements were within the scope of accreditation to ISO/IEC 17025:2005). The uncertainties of the certified values were estimated in accordance with the Guide to the Expression of Uncertainty in Measurement (GUM) and include uncertainties relating to possible inhomogeneity (Section 4), instability (Section 5) and characterisation (Section 6). The materials are intended for the calibration or quality control of real-time PCR measurements to identify Bt11 maize and quantify its mass fraction. As with any reference material, they can also be used for establishing control charts or for carrying out validation studies. The CRMs are available in glass bottles containing at least 1 g of dried maize seed powder, sealed under an atmosphere of argon. The minimum amount of sample to be used for extraction of the DNA is 200 mg.JRC.F.6-Reference Material

The certification of different mass fractions of the GM event 73496 in rapeseed powder: Certified Reference Materials ERM®-BF434a, ERM®-BF434b, ERM®-BF434c, ERM®-BF434d and ERM®-BF434e

This report describes the production of the set of Certified Reference Materials (CRMs) ERM-BF434a, b, c, d and e. These are matrix materials certified for their 73496 rapeseed mass fractions. The material has been produced following ISO Guide 34:2009. Genetically modified (GM) rapeseeds of the event 73496 and of a non-GM rapeseed variety were ground to obtain GM and non-GM base powders. Gravimetric mixtures of non-GM and GM rapeseed powder were prepared by dry-mixing. The between unit-heterogeneity has been quantified and stability during dispatch and stability during storage have been assessed in accordance with ISO Guide 35:2006. The certified value was obtained from the gravimetric preparations, taking into account the purity of the base materials and their respective water mass fraction. The certified values were confirmed by event-specific real-time PCR as an independent verification method (measurements within the scope of accreditation to ISO/IEC 17025:2005). Uncertainties of the certified values were calculated in compliance with the Guide to the Expression of Uncertainty in Measurement (GUM) and include uncertainties related to possible inhomogeneity (Section 4), instability (Section 5) and characterisation (Section 6). The materials are intended for the calibration or quality control of 73496 rapeseed identification and quantification methods. As any reference material, they can also be used for control charts or validation studies. The CRMs are available in glass vials containing at least 1 g of dried rapeseed powder closed under argon atmosphere. The minimum amount of sample to be used is 200 mg. The CRM has been accepted as European Reference Material (ERM®) after peer evaluation by the partners of the European Reference Materials consortium.JRC.D.2-Standards for Innovation and sustainable Developmen

Certification Report - The certification of different mass fractions of DAS-81419-2 in soya seed powder: Certified Reference Materials ERM®-BF437a, ERM®-BF437b, ERM®-BF437c, ERM®-BF437d and ERM®-BF437e

This report describes the production of a set of Certified Reference Materials (CRMs) ERM BF437a, b, c, d and e, which are certified for their DAS-81419-2 mass fractions. This material was produced following ISO Guide 34:2009 and is certified in accordance with ISO Guide 35:2006. Genetically modified (GM) seeds of the soya event DAS-81419-2 and seeds from a non-GM soya variety were milled to obtain GM and non-GM seed powders with a similar particle size distribution. Mixtures of non-GM and GM soya powder were prepared gravimetrically. The certified value was obtained from the gravimetric preparations, taking into account the genetic purity with respect to the event DAS-81419-2 of the two powder materials and their water mass fractions. The certified values were confirmed by event-specific real-time PCR as an independent verification method (measurements were within the scope of accreditation to ISO/IEC 17025:2005). Uncertainties of the certified values were calculated in accordance with the Guide to the Expression of Uncertainty in Measurement (GUM) and include uncertainties relating to possible inhomogeneity (Section 4), instability (Section 5) and characterisation (Section 6). The materials are intended for the calibration or quality control of real-time PCR measurements to identify DAS-81419-2 soya and/or quantify its mass fraction. As with any reference material, they can also be used for establishing control charts or for carrying out validation studies. The CRMs are available in glass vials containing at least 1 g of dried soya seed powder which were sealed under atmosphere of argon. The minimum amount of sample to be used is 200 mg. The CRMs were accepted as European Reference Material (ERM®) after peer evaluation by the partners of the European Reference Materials consortiumJRC.D.2-Standards for Innovation and sustainable Developmen

Certification of Reference Materials of Potato Powder with Different Mass Fractions of the Event AM04-1020 - Certified Reference Materials ERM®-BF430 (ERM®-BF430a, ERM®-BF430b, ERM®-BF430c,ERM®-BF430d, ERM®-BF430e)

This report describes the processing and certification of five potato powder Certified Reference Materials (CRMs) containing different mass fractions of genetically modified (GM) AM04-1020 potato (ERM-BF430a,b,c,d,e). The materials were processed and certified in 2011 by the European Commission, Joint Research Centre, The Institute for Reference Materials and Measurements (IRMM, Geel, Belgium), according to the principles of ISO Guide 34JRC.DG.D.2-Reference material

Certification of Reference Materials of Soya Seed Powder with different Mass Fractions of Genetically Modified 305423 Soya, Certified Reference Materials ERM-BF426 (ERM-BF426a, ERM-BF426b, ERM-BF426c, ERM-BF426d)

This report describes the processing and certification of four soya seed powder Certified Reference Materials (CRMs) containing different mass fractions of genetically modified (GM) 305423 soya (ERM-BF426a, b, c, d). The materials were processed and certified in 2007 by the European Commission, Directorate General Joint Research Centre, Institute for Reference Materials and Measurements (IRMM, Geel, Belgium), according to the principles of ISO Guide 34. The CRMs are intended to be used for quality control or calibration of methods for the quantification of the 305423 soya mass fraction in food and feed. The CRMs are available in glass bottles containing 1 g of dried soya powder closed under argon atmosphere. The minimum amount of sample to be used per analysis is 200 mg. The four CRMs have been accepted as European Reference Material (ERM) after peer evaluation by the partners of the European Reference Materials consortium.JRC.D.2-Reference material

Certification of Reference Materials of Maize Seed Powder containing Genetically Modified MON 810 Maize: Certified Reference Materials ERM®-BF413k (ERM®-BF413ak, ERM®-BF413ck, ERM®-BF413ek, ERM®-BF413gk)

The report describes the processing and certification of four maize seed powder Certified Reference Materials (CRMs) containing different mass fractions of genetically modified (GM) MON 810 maize (ERM-BF413ak, ck, ek, gk). The materials were processed and certified in 2007/2008 by the European Commission, Joint Research Centre, Institute for Reference Materials and Measurements IRMM, Geel, Belgium), according to the principles of ISO Guide 34. The four CRMs belonging to the ERM-BF413k series replace the earlier produced series ERM-BF413 which is exhausted. The four CRMs have been accepted as European Reference Material (ERM®) after peer evaluation by the partners of the European Reference Materials consortium.JRC.D.2-Reference material

CERTIFICATION REPORT The certification of different mass fractions of DP-ØØ4114-3 in maize seed powder Certified Reference Materials ERM®-BF439a, ERM®-BF439b, ERM®-BF439c, ERM®-BF439d and ERM®-BF439e

This report describes the production of a set of Certified Reference Materials (CRMs) ERM BF439a, b, c, d and e, which are certified for their 4114 maize event mass fractions. These materials were produced following ISO Guide 34:2009 and are certified in accordance with ISO Guide 35:2006. The materials are intended for the calibration or quality control of real-time PCR measurements to identify 4114 maize and/or quantify its mass fraction. As with any reference material, they can also be used for establishing control charts or for carrying out validation studies. The CRMs were accepted as European Reference Material (ERM®) after peer evaluation by the partners of the European Reference Materials consortium.JRC.D.2-Standards for Innovation and sustainable Developmen

The Certification of Reference Materials of Maize Seed Powder with different Mass Fractions of the Maize Event 3272. Certified Reference Materials ERM-BF420 (ERM-BF420a, ERM-BF420b, ERM-BF420c)

This report describes the processing and certification of maize Certified Reference Materials (CRMs) with different mass fractions of genetically modified (GM) Event 3272 maize seed powder (CRMs ERM-BF420a, ERM-BF420b, ERM-BF420c). The CRMs are intended to be used for the quality control or calibration of methods for the quantification of the Event 3272 maize mass fraction in food and feed. The GM maize mass fractions of ERM-BF420 were verified by an event-specific real-time polymerase chain reaction (rt-PCR) method. The CRMs are available in glass bottles containing 1 g of dried maize powder closed under argon atmosphere.JRC.D.2-Reference material

Gene co-expression architecture in peripheral blood in a cohort of remitted first-episode schizophrenia patients

A better understanding of schizophrenia subtypes is necessary to stratify the patients according to clinical attributes. To explore the genomic architecture of schizophrenia symptomatology, we analyzed blood co-expression modules and their association with clinical data from patients in remission after a first episode of schizophrenia. In total, 91 participants of the 2EPS project were included. Gene expression was assessed using the Clariom S Human Array. Weighted-gene co-expression network analysis (WGCNA) was applied to identify modules of co-expressed genes and to test its correlation with global functioning, clinical symptomatology, and premorbid adjustment. Among the 25 modules identified, six modules were significantly correlated with clinical data. These modules could be clustered in two groups according to their correlation with clinical data. Hub genes in each group showing overlap with risk genes for schizophrenia were enriched in biological processes related to metabolic processes, regulation of gene expression, cellular localization and protein transport, immune processes, and neurotrophin pathways. Our results indicate that modules with significant associations with clinical data showed overlap with gene sets previously identified in differential gene-expression analysis in brain, indicating that peripheral tissues could reveal pathogenic mechanisms. Hub genes involved in these modules revealed multiple signaling pathways previously related to schizophrenia, which may represent the complex interplay in the pathological mechanisms behind the disease. These genes could represent potential targets for the development of peripheral biomarkers underlying illness traits in clinical remission stages after a first episode of schizophrenia

The certification of different mass fractions of DAS-40278-9 in maize seed powder: ERM®-BF433a,ERM®-BF433b, ERM®-BF433c and ERM®-BF433d

This report describes the production of a set of Certified Reference Materials (CRMs) ERM-BF433a, b, c and d, matrix materials certified for their DAS-40278-9 mass fractions. The material has been produced following ISO Guide 34:2009. Genetically modified (GM) seeds of the maize event DAS-40278-9 and of a non-GM maize variety were ground to obtain GM and non-GM base powders. Gravimetric mixtures of non-GM and GM maize powder were prepared by dry-mixing. Between unit-heterogeneity has been quantified and stability during dispatch and storage have been assessed in accordance with ISO Guide 35:2006. The certified value was obtained from the gravimetric preparations, taking into account the purity of the base materials and their respective water mass fraction. The certified values were confirmed by event-specific real-time PCR as independent verification method (measurements within the scope of accreditation to ISO/IEC 17025:2005. Uncertainties of the certified values were calculated in compliance with the Guide to the Expression of Uncertainty in Measurement (GUM) and include uncertainties related to possible heterogeneity, instability, and characterisation. The materials are intended for the calibration or quality control of methods. As any reference material, they can also be used for control charts or validation studies. The CRMs are available in glass vials containing at least 1 g of dried maize seed powder, closed under argon atmosphere. The minimum amount of sample to be used is 200 mg. The CRM has been accepted as European Reference Material (ERM®) after peer evaluation by the partners of the European Reference Materials consortium.JRC.D.2-Standards for Innovation and sustainable Developmen