Phosphorylation of CENP-A on serine 7 does not control centromere function

CENP-A is the histone H3 variant necessary to specify the location of all eukaryotic centromeres via its CENP-A targeting domain and either one of its terminal regions. In humans, several post-translational modifications occur on CENP-A, but their role in centromere function remains controversial. One of these modifications of CENP-A, phosphorylation on serine 7, has been proposed to control centromere assembly and function. Here, using gene targeting at both endogenous CENP-A alleles and gene replacement in human cells, we demonstrate that a CENP-A variant that cannot be phosphorylated at serine 7 maintains correct CENP-C recruitment, faithful chromosome segregation and long-term cell viability. Thus, we conclude that phosphorylation of CENP-A on serine 7 is dispensable to maintain correct centromere dynamics and function

CENP-A Is Dispensable for Mitotic Centromere Function after Initial Centromere/Kinetochore Assembly

Human centromeres are defined by chromatin containing the histone H3 variant CENP-A assembled onto repetitive alphoid DNA sequences. By inducing rapid, complete degradation of endogenous CENP-A, we now demonstrate that once the first steps of centromere assembly have been completed in G1/S, continued CENP-A binding is not required for maintaining kinetochore attachment to centromeres or for centromere function in the next mitosis. Degradation of CENP-A prior to kinetochore assembly is found to block deposition of CENP-C and CENP-N, but not CENP-T, thereby producing defective kinetochores and failure of chromosome segregation. Without the continuing presence of CENP-A, CENP-B binding to alphoid DNA sequences becomes essential to preserve anchoring of CENP-C and the kinetochore to each centromere. Thus, there is a reciprocal interdependency of CENP-A chromatin and the underlying repetitive centromere DNA sequences bound by CENP-B in the maintenance of human chromosome segregation

Evaluation of peak-picking algorithms for protein mass spectrometry

Peak picking is an early key step in MS data analysis. We compare three commonly used approaches to peak picking and discuss their merits by means of statistical analysis. Methods investigated encompass signal-to-noise ratio, continuous wavelet transform, and a correlation-based approach using a Gaussian template. Functionality of the three methods is illustrated and discussed in a practical context using a mass spectral data set created with MALDI-TOF technology. Sensitivity and specificity are investigated using a manually defined reference set of peaks. As an additional criterion, the robustness of the three methods is assessed by a perturbation analysis and illustrated using ROC curves

Molecular dynamics simulations of lead clusters

Molecular dynamics simulations of nanometer-sized lead clusters have been performed using the Lim, Ong and Ercolessi glue potential (Surf. Sci. {\bf 269/270}, 1109 (1992)). The binding energies of clusters forming crystalline (fcc), decahedron and icosahedron structures are compared, showing that fcc cuboctahedra are the most energetically favoured of these polyhedral model structures. However, simulations of the freezing of liquid droplets produced a characteristic form of ``shaved'' icosahedron, in which atoms are absent at the edges and apexes of the polyhedron. This arrangement is energetically favoured for 600-4000 atom clusters. Larger clusters favour crystalline structures. Indeed, simulated freezing of a 6525-atom liquid droplet produced an imperfect fcc Wulff particle, containing a number of parallel stacking faults. The effects of temperature on the preferred structure of crystalline clusters below the melting point have been considered. The implications of these results for the interpretation of experimental data is discussed.Comment: 11 pages, 18 figues, new section added and one figure added, other minor changes for publicatio

Unknowns after the SNO Charged-Current Measurement

We perform a model-independent analysis of solar neutrino flux rates including the recent charged-current measurement at the Sudbury Neutrino Observatory (SNO). We derive a universal sum rule involving SNO and SuperKamiokande rates, and show that the SNO neutral-current measurement can not fix the fraction of solar $\nu_e$ oscillating to sterile neutrinos. The large uncertainty in the SSM $^8$B flux impedes a determination of the sterile neutrino fraction.Comment: Version to appear in PRL; includes analysis with anticipated SNO NC measuremen

Investigation of DC-8 nacelle modifications to reduce fan-compressor noise in airport communities. Part 4 - Flight acoustical and performance evaluations, for period May 1967 - October 1969

Flight acoustical and performance evaluations of DC 8 nacelle modifications to reduce fan-compressor noise in airport communitie

Violation of the Equivalence Principle in the light of the SNO and SK solar neutrino results

The SNO result on charged current deuteron disintegration, the SuperKamiokande 1258-day data on electron scattering, and other solar neutrino results are used to revisit the model of neutrino oscillations driven by a violation of the equivalence principle. We use a chisq minimization technique to examine oscillation between the nu(e) and another active neutrino, both massless, and find that within the Standard Solar Model the fit to the SNO and SuperKamiokande spectra are moderately good while a very good fit is obtained when the absolute normalizations of the 8B and hep neutrino fluxes are allowed to vary. The best fit prefers large, but not maximal, mixing, essentially no hep neutrinos, and a 40% reduction in the 8B neutrino flux. The fit to the total rates from the different experiments is not encouraging but when the rates and spectra are considerd together the situation is much improved. We remark on the expectations of the VEP model for the neutral current measurements at SNO.Comment: Latex, 11 pages (incl. 1 postscript figure

Maximal quantum yield of photosynthesis in the northwestern Sargasso Sea

The magnitude and variability of the maximal quantum yield of photosynthesis were examined in the northwestern Sargasso Sea in April 1985. Maximal quantum yield was calculated from light-limited photosynthetic rates and spectrally-weighted absorption coefficients. The absorption by total particulates collected on a glass fiber filter was partitioned into two components, one associated with living phytoplankton and one associated with other absorbing particles. Two types of maximal quantum yield were calculated: one from the absorption by total particulates and one from the absorption by the phytoplanktonic component alone. Maximal quantum yield calculated from absorption by total particulates was low [0.014 to 0.071 mol C (mol photons)–1] and decreased as the proportion of absorption due to the non phytoplanktonic particles increased. The phytoplanktonic maximal quantum yield was higher [0.033 to 0.102 mol C (mol photons)–1] and varied by a factor of two over a period of two weeks during and following a spring bloom. Use of the phytoplanktonic component of absorption to calculate maximal quantum yield allowed analysis of changes in maximal quantum yield as a function of changes in phytoplankton physiology rather than changes in the amount of absorption by particulate detritus. The pattern of variation in quantum yield was related to nitrogen flux; these data suggest that maximal quantum yield can be predicted from environmental conditions on a regional or seasonal basis

Next Generation Differential GPS Architecture

The United States Coast Guard is engaged in a project to re-capitalize Reference Station (RS) and Integrity Monitor (IM) equipment used in the Nationwide Differential Global Position System (NDGPS). The Coast Guard in partnership with industry is developing a new software application to run on an open architecture platform as a replacement for legacy equipment. Present commercially available off-the-shelf Differential Global Positioning System (DGPS) RS and IM equipment lacks the open architecture required to support long term goals and future system improvements. The utility of the proposed new hardware architecture and software application is impressive - nearly every aspect of performance and supportability significantly exceeds that of the legacy architecture. The flexibility of the new hardware and software architectures complement each other to offer promising possibilities for the future. For example, the new hardware architecture uses Ethernet for internal and external site equipment communications. Each Local Area Network (LAN) will be equipped with a router and two 24 port switches. Various levels of password protection are provided to manage security both locally and remotely. While the new software application directly supports the legacy RS-232/422 interfaces to devices such as GPS receivers, a system design goal includes the ability to directly address each device from NCS. With the use of TCP/IP to RS-232/422 port server devices, the system can meet these forward reaching goals while supporting legacy equipment. New system capabilities include remote software management, remote hardware configuration management, and flexible options for management of licenses. The new configurable RS and IM architecture is a PCbased emulation of legacy reference station and integrity monitor equipment. It supports fluid growth and exploitation of new signals, formats, and technology as they become available, while remaining backward compatible with legacy architecture and user equipment. Examples of new capabilities include enhanced data management & anomaly analysis, universal On Change Reference Station Integrity Monitor (RSIM) message scheduling, improved satellite clock handling, additional observation interval modes, and Range Rate Correction monitoring in the IM. Engineering initiatives under development such as implementation of pre-broadcast integrity are also presented. This paper details challenges and goals that drove software and hardware design approaches destined to become the backbone of the Next Generation Differential GPS Architecture. Functional differences between legacy and next generation operation are explored. The new DGPS system architecture will allow the USCG radiobeacon system to continue to deliver and improve navigation and positioning services to our nation and its territories. Reprinted with permission from The Institute of Navigation (http://ion.org/) and The Proceedings of the 18th International Technical Meeting of the Satellite Division of The Institute of Navigation, (pp. 816-826). Fairfax, VA: The Institute of Navigation

Entropic effects on the Size Evolution of Cluster Structure

We show that the vibrational entropy can play a crucial role in determining the equilibrium structure of clusters by constructing structural phase diagrams showing how the structure depends upon both size and temperature. These phase diagrams are obtained for example rare gas and metal clusters.Comment: 5 pages, 3 figure