Chemical stability study of vitamins thiamine, riboflavin, pyridoxine and ascorbic acid in parenteral nutrition for neonatal use

<p>Abstract</p> <p>Background</p> <p>The objective of this work was to study the vitamins B₁, B₂, B₆and C stability in a pediatric formulation containing high amounts of calcium in the presence of organic phosphate, amino acids, glucose, sodium chloride, magnesium sulfate, pediatric vitamins and trace elements under different conditions using developed and validated analytical methods.</p> <p>Methods</p> <p>The study was carried out during 72 h with formulations packaged in recommended storage temperature (4°C) and 25°C, with and without photoprotection.</p> <p>Results</p> <p>The results showed that the methodologies used for assessing the chemical stability of vitamins B₁, B₂, B₆and C in the formulation were selective, linear, precise and accurate. The vitamins could be considered stable in the formulation during the three days of study if stored at 4°C. When stored at 25°C vitamin C presented instability after 48 h.</p> <p>Conclusion</p> <p>The pediatric formulation containing high amount of calcium in the presence of organic phosphate, amino acids, glucose, sodium chloride, magnesium sulphate, pediatric vitamins and trace elements packaged in bag-type trilaminate presented a shelf life of the 72 h, when maintained under refrigeration, between 2°C and 8°C. This shelf life was measured considering the vitamins studied. Further studies are needed including all the vitamins present in this formulation.</p

Rhodium(I) complexes containing the enolate of N-acetyl-3-butanoyltetramic acid (Habta) and the crystal structure of [Rh(abta)P(OPh)(3)(2)]

Reaction of N-acetyl-3-butanoyltetramic acid (Habta) (N-acetyl-3-butanoyl-1,5-dihydro-4-hydroxy-2H-pyrrol-2-one) with [Rh(acac)(CO)(2)] (acac = acetylacetonate) in a 1:1 ratio gave [Rh(abta)(CO)(2)] 1 which underwent displacement of CO by either P(OPh)(3) or PPh(3) to give [Rh(abta)(CO)L] [L = P(OPh)(3) or 2 PPh(3) 4] and [Rh(abta)P(OPh)(3)(2)] 3; the reaction of 4 with PPh(3) gave the five-co-ordinate complex [Rh(abta)(CO)(PPh(3))(2)] 5. The solid-state structure of 3 has been determined by X-ray diffraction. It shows that rhodium adopts a slightly distorted square-planar geometry with the abta enolate ligand adopting an O,O’ mode of co-ordination via the functionalities associated with C-4 and the acyl group at C-3 in the pyrrolidine ring. Under (CO)-C-13, 3 is in equilibrium with 2 and 1 as shown by C-13 NMR spectroscopy. No evidence has been found for the formation of five-co-ordinate complexes through the addition of P(OPh)(3) to 2