2 research outputs found
Evaluation of HA negatively charged membranes in the recovery of human adenoviruses and hepatitis A virus in different water matrices
Evaluation of HA negatively charged membranes in the recovery of human adenoviruses and hepatitis A virus in different water matrices
Human adenoviruses (HAdV) and hepatitis A virus (HAV) are shed in the
faeces and consequently may be present in environmental waters,
resulting in an increase in pathogen concentration that can affect
water quality and human health. The aim of this study was to evaluate
an adsorption-elution method which utilizes negatively charged membrane
HA to determine the efficient recovery of HAdV and HAV from different
water matrices and to combine this procedure with a qualitative
molecular method (nested RT-PCR and nested PCR). The best efficiency
recovery was achieved in distilled water and treated wastewater
effluent (100%) for both viruses and in recreational lagoon water for
HAV (100%). The efficiency recovery was 10% for HAdV and HAV in
seawater and 10% for HAdV in lagoon water. The viral detection limit by
nested PCR for HAV in water samples ranged between 20-0.2 FFU/mL and
250 and 25 TCID50/mL for HAdV. In conclusion, these results suggest
that the HA negatively charged membranes vary their efficiency for
recovery of viral concentration depending upon the types of both
enteric viruses and water matrices