Expression of p89c-Mybex9b, an alternatively spliced form of c-Myb, is required for proliferation and survival of p210BCR/ABL-expressing cells

The c-Myb gene encodes the p75c-Myb isoform and less-abundant proteins generated by alternatively spliced transcripts. Among these, the best known is pc-Mybex9b, which contains 121 additional amino acids between exon 9 and 10, in a domain involved in protein–protein interactions and negative regulation. In hematopoietic cells, expression of pc-Mybex9b accounts for 10–15% of total c-Myb; these levels may be biologically relevant because modest changes in c-Myb expression affects proliferation and survival of leukemic cells and lineage choice and frequency of normal hematopoietic progenitors. In this study, we assessed biochemical activities of pc-Mybex9b and the consequences of perturbing its expression in K562 and primary chronic myeloid leukemia (CML) progenitor cells. Compared with p75c-Myb, pc-Mybex9b is more stable and more effective in transactivating Myb-regulated promoters. Ectopic expression of pc-Mybex9b enhanced proliferation and colony formation and reduced imatinib (IM) sensitivity of K562 cells; conversely, specific downregulation of pc-Mybex9b reduced proliferation and colony formation, enhanced IM sensitivity of K562 cells and markedly suppressed colony formation of CML CD34+ cells, without affecting the levels of p75c-Myb. Together, these studies indicate that expression of the low-abundance pc-Mybex9b isoform has an important role for the overall biological effects of c-Myb in BCR/ABL-transformed cells

Privacy Protection in Tourism: Where We Are and Where We Should Be Heading For

The link between information privacy concerns and privacy behaviours has been a focus of extensive investigation in various disciplines. However, little attention has been devoted to this issue in the tourism literature. Spurred by technological development and shaped by tourism-related environments, emerging privacy issues call for comprehensive yet context-specific studies to ensure tourists are making beneficial privacy choices. This paper first presents a comprehensive review of state-of-the-art research on privacy concerns and behaviours. Then, it suggests a list of overarching research priorities, merging social and technical aspects of privacy protection approaches as they apply to tourism. The priorities include research to measure tourists’ privacy concerns, explore specific biases in tourists’ privacy decisions, experiment with privacy nudges, and explore how to integrate privacy nudges in system design. Thus, this paper contributes to guiding the direction of future research on privacy protection in tourism