33 research outputs found
Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663
<p>Abstract</p> <p>Background</p> <p>Differentiation of the acute myeloid leukemia (AML) cell line HL-60 can be induced by all trans-retinoic acid (ATRA); however, the mechanism regulating this process has not been fully characterized.</p> <p>Methods</p> <p>Using bioinformatics and <it>in vitro </it>experiments, we identified the microRNA gene expression profile of HL-60 cells during ATRA induced granulocytic differentiation.</p> <p>Results</p> <p>Six microRNAs were upregulated by ATRA treatment, miR-663, miR-494, miR-145, miR-22, miR-363* and miR-223; and three microRNAs were downregulated, miR-10a, miR-181 and miR-612. Additionally, miR-663 expression was regulated by ATRA. We used a lentivirus (LV) backbone incorporating the spleen focus forming virus (SFFV-F) promoter to drive miR-663 expression, as the CMV (Cytomegalovirus) promoter is ineffective in some lymphocyte cells. Transfection of LV-miR-663 induced significant HL-60 cell differentiation <it>in vitro</it>.</p> <p>Conclusions</p> <p>Our results show miR-663 may play an important role in ATRA induced HL-60 cell differentiation. Lentivirus delivery of miR-663 could potentially be used directly as an anticancer treatment in hematological malignancies</p
Lead induces oxidative stress and phenotypic markers of apoptosis in Saccharomyces cerevisiae
In the present work, the mode of cell death induced by Pb in Saccharomyces cerevisiae was studied.
Yeast cells Pb-exposed, up to 6 h, loss progressively the
capacity to proliferate and maintained the membrane
integrity evaluated by the fluorescent probes bis(1,3-
dibutylbarbituric acid trimethine oxonol) and propidium
iodide. Pb-induced death is an active process, requiring the
participation of cellular metabolism, since the simultaneous
addition of cycloheximide attenuated the loss of cell
proliferation capacity. Cells exposed to Pb accumulated
intracelullarly reactive oxygen species (ROS), evaluated by
2′,7′-dichlorodihydrofluorescein diacetate. The addition of
ascorbic acid (a ROS scavenger) strongly reduced the
oxidative stress and impaired the loss of proliferation
capacity in Pb-treated cells. Pb-exposed cells displayed
nuclear morphological alterations, like chromatin fragmentation, as revealed by diaminophenylindole staining. Together,
the data obtained indicate that yeast cells exposition
to 1 mmol/l Pb results in severe oxidative stress which can
be the trigger of programmed cell death by apoptosis